Detailed information NTG1077734

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACJGE4_RS12640
• Genome accession: NZ_CP174519
• Coordinates: 2599595..2600209 (-)
• Length: 204 a.a.
• NCBI ID: WP_043021637.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain JD-3
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2594595..2605209

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACJGE4_RS12610 (ACJGE4_12610)	rpsT	2594898..2595164 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACJGE4_RS12615 (ACJGE4_12615)	holA	2595181..2596222 (-)	1042	Protein_2438	DNA polymerase III subunit delta	-
ACJGE4_RS12620 (ACJGE4_12620)	-	2596262..2596413 (-)	152	Protein_2439	hypothetical protein	-
ACJGE4_RS12625 (ACJGE4_12625)	-	2596454..2596588 (+)	135	WP_003152870.1	YqzM family protein	-
ACJGE4_RS12630 (ACJGE4_12630)	comEC	2596607..2598958 (-)	2352	WP_094031847.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACJGE4_RS12635 (ACJGE4_12635)	-	2598959..2599528 (-)	570	WP_094031848.1	ComE operon protein 2	-
ACJGE4_RS12640 (ACJGE4_12640)	comEA	2599595..2600209 (-)	615	WP_043021637.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACJGE4_RS12645 (ACJGE4_12645)	comER	2600268..2601089 (+)	822	WP_012118027.1	late competence protein ComER	-
ACJGE4_RS12650 (ACJGE4_12650)	-	2601158..2601895 (-)	738	WP_076424998.1	class I SAM-dependent methyltransferase	-
ACJGE4_RS12655 (ACJGE4_12655)	rsfS	2601892..2602248 (-)	357	WP_007408260.1	ribosome silencing factor	-
ACJGE4_RS12660 (ACJGE4_12660)	yqeK	2602266..2602826 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACJGE4_RS12665 (ACJGE4_12665)	-	2602816..2603385 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
ACJGE4_RS12670 (ACJGE4_12670)	yhbY	2603396..2603686 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
ACJGE4_RS12675 (ACJGE4_12675)	aroE	2603680..2604522 (-)	843	WP_094031849.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22165.31 Da
• Isoelectric Point: 6.4888

>NTDB_id=1077734 ACJGE4_RS12640 WP_043021637.1 2599595..2600209(-) (comEA) [Bacillus velezensis strain JD-3]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1077734 ACJGE4_RS12640 WP_043021637.1 2599595..2600209(-) (comEA) [Bacillus velezensis strain JD-3]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363