Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACI6TB_RS01200 Genome accession   NZ_CP174270
Coordinates   256288..257703 (+) Length   471 a.a.
NCBI ID   WP_406812353.1    Uniprot ID   -
Organism   Histophilus somni strain HSNMG-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 251288..262703
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI6TB_RS01185 (ACI6TB_01185) clpX 252704..253948 (-) 1245 WP_075293630.1 ATP-dependent protease ATP-binding subunit ClpX -
  ACI6TB_RS01190 (ACI6TB_01190) clpP 253964..254545 (-) 582 WP_075293629.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -
  ACI6TB_RS01195 (ACI6TB_01195) tig 254631..255929 (-) 1299 WP_011609585.1 trigger factor -
  ACI6TB_RS01200 (ACI6TB_01200) pilB 256288..257703 (+) 1416 WP_406812353.1 GspE/PulE family protein Machinery gene
  ACI6TB_RS01205 (ACI6TB_01205) - 257737..258996 (-) 1260 WP_012341088.1 HlyC/CorC family transporter -
  ACI6TB_RS01210 (ACI6TB_01210) - 259066..259863 (-) 798 WP_406812354.1 inner membrane protein YpjD -
  ACI6TB_RS01215 (ACI6TB_01215) ffh 260009..261385 (+) 1377 WP_132994679.1 signal recognition particle protein -
  ACI6TB_RS01220 (ACI6TB_01220) tnpA 261475..261891 (-) 417 WP_075321156.1 IS200/IS605 family transposase -

Sequence

Protein


Download         Length: 471 a.a.        Molecular weight: 53852.86 Da        Isoelectric Point: 5.9894

>NTDB_id=1076658 ACI6TB_RS01200 WP_406812353.1 256288..257703(+) (pilB) [Histophilus somni strain HSNMG-1]
MIIPQSEDMSPIVMSIDGDTYEITLHLWQRNQQQSQILLRYFAIPLQENEQTLWLGVDSLNNISACETFAFLYGKIVEPV
LLNNQLLKQLLQNLSPQQNNMFVEEQPINQYVAEHRIEVTEKSDEPVIQLLDHIFENALKQNVSDIHIEPQMNCLQIRFR
IDGILQCQSPLPLSLSKRILSRLKLLAKLDISETRLPQDGRFHFKTTFSDILDFRLSTLPTNMGEKAVLRLQQNKPVQLS
FAELGMTENQQKSFKQALSQPQGLILVTGPTGSGKSISLYTALQWLNDKHKHIMTAEDPIEIELNGIIQCQINPQIGLDF
SRLLRTFLRQDPDIIMLGEIRDNESAIMALRAAQTGHLVLSTLHTNDAPSAVSRLLQLGVKQHEIDNSLLLVIAQRLVRK
KCPHTENENCTCHQKYQGRIGVYQFLQPHLMDNHICYQTDYAHLRESAMEKVRLEITDLAEVDRVIGQSSE

Nucleotide


Download         Length: 1416 bp        

>NTDB_id=1076658 ACI6TB_RS01200 WP_406812353.1 256288..257703(+) (pilB) [Histophilus somni strain HSNMG-1]
ATGATAATTCCTCAATCTGAAGATATGTCTCCTATAGTGATGTCGATCGATGGAGATACTTATGAAATAACACTGCACTT
ATGGCAAAGAAATCAGCAACAATCCCAAATTCTGCTACGCTATTTTGCCATTCCTTTACAGGAAAATGAACAAACGTTAT
GGTTAGGCGTTGATAGTCTAAATAATATTAGTGCCTGTGAAACATTTGCATTTTTGTATGGAAAAATTGTAGAACCGGTT
TTACTTAATAACCAATTACTTAAACAGTTACTACAAAACTTATCACCACAGCAAAATAATATGTTCGTTGAAGAGCAACC
TATAAATCAATATGTAGCCGAGCATCGTATAGAAGTAACTGAAAAGTCTGATGAACCCGTGATTCAACTTTTAGATCACA
TTTTTGAAAATGCATTAAAGCAAAATGTATCAGATATTCATATTGAACCGCAAATGAATTGCTTGCAAATACGTTTCAGA
ATAGACGGTATTTTACAATGCCAATCGCCTCTTCCGCTTTCGCTGAGTAAGCGTATTCTTTCACGTTTAAAGTTACTGGC
CAAATTGGATATAAGCGAAACACGGTTACCTCAAGATGGACGATTTCATTTTAAAACAACATTTTCTGACATCCTTGATT
TTCGTTTATCAACCCTTCCGACGAATATGGGTGAAAAAGCCGTGTTGCGTTTACAGCAAAATAAACCTGTACAGTTGAGT
TTTGCGGAACTCGGCATGACAGAAAACCAGCAAAAATCATTCAAACAGGCACTATCTCAACCCCAAGGACTCATTTTAGT
GACAGGCCCAACAGGGAGTGGAAAAAGTATCTCACTTTACACTGCACTTCAATGGCTGAATGATAAGCACAAACATATTA
TGACAGCGGAAGATCCGATAGAAATTGAATTGAACGGTATCATTCAATGTCAAATAAATCCGCAGATCGGGTTAGATTTT
AGCCGACTGTTAAGAACTTTTCTTCGTCAAGATCCTGACATTATTATGTTGGGTGAAATTCGGGATAACGAAAGTGCAAT
AATGGCATTAAGAGCAGCACAAACCGGACATCTTGTTCTCTCAACTTTACATACAAATGATGCTCCGTCGGCAGTCTCAC
GCTTGCTTCAATTGGGGGTAAAGCAACATGAAATTGACAATAGTTTATTATTGGTCATCGCTCAACGCTTGGTACGAAAA
AAATGTCCACATACAGAAAATGAAAATTGCACTTGTCATCAGAAATATCAAGGGCGAATTGGTGTTTATCAATTTTTACA
ACCGCATCTAATGGATAATCATATTTGCTACCAAACGGATTATGCTCATTTGCGTGAAAGTGCAATGGAAAAAGTGCGGT
TAGAAATAACAGATTTAGCAGAAGTGGATAGAGTTATTGGACAAAGTAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(134-400)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-98)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

68.33

97.877

0.669
  pilB Haemophilus influenzae Rd KW20

67.609

97.665

0.66
  pilB Glaesserella parasuis strain SC1401

57.333

95.541

0.548
  pilB Vibrio campbellii strain DS40M4

40.041

100

0.41
  pilB Vibrio cholerae strain A1552

39.419

100

0.403
  pilB Vibrio parahaemolyticus RIMD 2210633

38.477

100

0.397
  pilB Legionella pneumophila strain ERS1305867

37.045

100

0.389
  pilB Acinetobacter baumannii D1279779

37.681

100

0.386
  pilB Acinetobacter baylyi ADP1

36.072

100

0.382
  pilF Neisseria gonorrhoeae MS11

36.076

100

0.363

Multiple sequence alignment