Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACI6TB_RS00710 Genome accession   NZ_CP174270
Coordinates   156795..157253 (+) Length   152 a.a.
NCBI ID   WP_136121890.1    Uniprot ID   -
Organism   Histophilus somni strain HSNMG-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 151795..162253
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI6TB_RS00680 (ACI6TB_00680) ispB 151816..152787 (-) 972 WP_075321735.1 octaprenyl diphosphate synthase -
  ACI6TB_RS00685 (ACI6TB_00685) rplU 153028..153339 (+) 312 WP_011608399.1 50S ribosomal protein L21 -
  ACI6TB_RS00690 (ACI6TB_00690) rpmA 153360..153617 (+) 258 WP_011608400.1 50S ribosomal protein L27 -
  ACI6TB_RS00695 (ACI6TB_00695) - 153753..154673 (+) 921 WP_012340377.1 DMT family transporter -
  ACI6TB_RS00700 (ACI6TB_00700) cgtA 154683..155861 (+) 1179 WP_406812325.1 Obg family GTPase CgtA -
  ACI6TB_RS00705 (ACI6TB_00705) ampD 156103..156672 (-) 570 WP_012340392.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACI6TB_RS00710 (ACI6TB_00710) pilA 156795..157253 (+) 459 WP_136121890.1 prepilin peptidase-dependent pilin Machinery gene
  ACI6TB_RS00715 (ACI6TB_00715) artP 157399..158136 (+) 738 WP_012340409.1 arginine ABC transporter ATP-binding protein ArtP -
  ACI6TB_RS00720 (ACI6TB_00720) - 158154..158879 (+) 726 WP_012340415.1 transporter substrate-binding domain-containing protein -
  ACI6TB_RS00725 (ACI6TB_00725) artQ 158884..159555 (+) 672 WP_011608407.1 arginine ABC transporter permease ArtQ -
  ACI6TB_RS00730 (ACI6TB_00730) artM 159566..160249 (+) 684 WP_406812326.1 arginine ABC transporter permease ArtM -
  ACI6TB_RS00735 (ACI6TB_00735) - 160355..160606 (-) 252 WP_233120535.1 DUF5339 domain-containing protein -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 15903.18 Da        Isoelectric Point: 8.4638

>NTDB_id=1076652 ACI6TB_RS00710 WP_136121890.1 156795..157253(+) (pilA) [Histophilus somni strain HSNMG-1]
MKITYIFSSVKKGFTLIELMIVIAIVAILATIAIPSYQNYTKKAAITEILQASAPYRAEVELCVYNNDAPTNCNAGTNGI
QAAINTPKGKVKSVSVQGGAITVTGNGNLKDITYTLTPTGDASTGVTWSANCNNKADLFPAGFCSSTSTNSP

Nucleotide


Download         Length: 459 bp        

>NTDB_id=1076652 ACI6TB_RS00710 WP_136121890.1 156795..157253(+) (pilA) [Histophilus somni strain HSNMG-1]
ATGAAAATAACCTATATTTTTTCATCAGTTAAAAAAGGATTTACTCTCATAGAACTGATGATTGTTATTGCTATTGTGGC
AATTCTTGCAACCATTGCAATTCCGTCATACCAAAATTATACAAAAAAAGCTGCAATTACAGAGATATTGCAAGCTTCAG
CTCCTTATCGTGCAGAAGTTGAGCTATGCGTGTATAACAATGATGCACCGACAAATTGTAATGCTGGAACAAATGGAATT
CAAGCGGCAATCAATACACCAAAAGGTAAAGTAAAATCAGTTAGCGTGCAAGGTGGAGCCATTACTGTTACAGGGAATGG
AAATCTTAAAGATATTACTTATACCTTAACACCAACAGGCGATGCTTCAACCGGCGTTACTTGGAGTGCAAATTGCAATA
ATAAGGCCGATCTATTTCCTGCCGGATTTTGCTCTTCTACGTCTACAAACTCTCCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(11-33)

Pilin (bacterial filament) (Pilin)

(43-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

64.626

96.711

0.625
  pilA Haemophilus influenzae Rd KW20

62.585

96.711

0.605
  pilA Glaesserella parasuis strain SC1401

49.677

100

0.507
  pilA Ralstonia pseudosolanacearum GMI1000

35.714

100

0.395
  pilA Vibrio campbellii strain DS40M4

38.356

96.053

0.368

Multiple sequence alignment