Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACJEH2_RS12600 Genome accession   NZ_CP174235
Coordinates   2818399..2820495 (+) Length   698 a.a.
NCBI ID   WP_110539734.1    Uniprot ID   -
Organism   Legionella pneumophila strain SPF590     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2813399..2825495
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJEH2_RS12575 (ACJEH2_12575) ravI 2814161..2815168 (-) 1008 WP_011215093.1 Dot/Icm T4SS effector RavI -
  ACJEH2_RS12580 (ACJEH2_12580) pilM 2815585..2816649 (+) 1065 WP_011215094.1 type IV pilus assembly protein PilM Machinery gene
  ACJEH2_RS12585 (ACJEH2_12585) pilN 2816653..2817201 (+) 549 WP_010946663.1 PilN domain-containing protein Machinery gene
  ACJEH2_RS12590 (ACJEH2_12590) pilO 2817215..2817814 (+) 600 WP_011215096.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACJEH2_RS12595 (ACJEH2_12595) pilP 2817811..2818395 (+) 585 WP_027266404.1 pilus assembly protein PilP Machinery gene
  ACJEH2_RS12600 (ACJEH2_12600) pilQ 2818399..2820495 (+) 2097 WP_110539734.1 type IV pilus secretin PilQ Machinery gene
  ACJEH2_RS12605 (ACJEH2_12605) aroK 2820941..2821468 (+) 528 WP_010946667.1 shikimate kinase AroK -
  ACJEH2_RS12610 (ACJEH2_12610) aroB 2821455..2822564 (+) 1110 WP_408073318.1 3-dehydroquinate synthase -
  ACJEH2_RS12615 (ACJEH2_12615) - 2822561..2824012 (+) 1452 WP_070322083.1 SPOR domain-containing protein -
  ACJEH2_RS12620 (ACJEH2_12620) - 2824117..2824548 (+) 432 WP_011215101.1 universal stress protein -

Sequence

Protein


Download         Length: 698 a.a.        Molecular weight: 77489.77 Da        Isoelectric Point: 9.5345

>NTDB_id=1076107 ACJEH2_RS12600 WP_110539734.1 2818399..2820495(+) (pilQ) [Legionella pneumophila strain SPF590]
MRKIIAILILISASLSMAYSKDNSLISVKVLPLPENRVRIDFQFSKPLKQLPASFITQKPPRLVLDFISTDMQIPSDQKT
KTIQLGSLHSYNIVAVGDRVRAILELDRSVSYSGSTAGSVYSLVLNGKSNDLFENSKEVFITNQVVNAKHEIKRIDFRGI
EKQGGRVLIDVSDTGIPIDVTQVGKEIVVNFLNTKIPLNLMKRYDVSDFHSPVQIITMQQEGKKVRMTILSKGDYGHFVY
QVNKQFMVDVFPLTAEEIRQAKLKKQVFTGKRISLNFQNISIRAVLQLLADFTGINMVVSDKVQGDITLRLNDIPWDQAL
DIILTTQGLDKRRKGNVMLIDTKASMDKMEEEQLKSQQTIEKLEPIRSDLIQINYAKAADISVLIKDKQNSLLSDRGKIS
VDARTNTIWIQDTGTKIEEVRELIKQLDIPVKQVLIEARIVEVTKDFSQDIGIRWGVSRPTHLSGTLEGANELARGTAPA
NVVPLERRLNLDLAAAPLTGATPASVGIALAKLNDNILLDLELSALESEGRAELISSPRLITTNQQAAVIESGEEIPYQE
ATSSGATAVAFKKAVLSLKVTPQITPDGKILMDLQINQDTPSPQTFLGVPAIITKEIQTNVLVNNGQTIVLGGIYKQDKN
KVINRIPFFGELPVVGILFSNRQITLKNEELLIFITPRIITNALSITTIEGREKDVYK

Nucleotide


Download         Length: 2097 bp        

>NTDB_id=1076107 ACJEH2_RS12600 WP_110539734.1 2818399..2820495(+) (pilQ) [Legionella pneumophila strain SPF590]
GTGAGAAAAATTATCGCGATTTTGATATTAATTAGTGCCAGTTTGAGTATGGCATATTCTAAAGATAATTCGCTCATTTC
AGTAAAAGTATTGCCATTGCCCGAAAACAGAGTGCGTATTGATTTTCAATTTTCCAAGCCCTTAAAGCAGTTGCCTGCAA
GCTTTATTACTCAAAAGCCGCCCCGTTTGGTGTTAGATTTTATCAGCACTGATATGCAAATTCCTTCGGATCAGAAAACT
AAAACTATTCAATTAGGCTCATTACACAGTTATAACATTGTTGCTGTTGGGGATAGAGTAAGGGCCATATTGGAACTGGA
TCGTTCAGTATCCTATTCTGGCAGTACGGCGGGTTCCGTTTATAGCCTTGTCCTGAATGGTAAAAGCAATGATTTATTTG
AAAATAGCAAGGAGGTATTCATAACGAACCAGGTTGTGAACGCCAAGCATGAAATTAAAAGAATTGATTTTCGTGGTATA
GAAAAGCAGGGCGGTAGAGTTTTAATTGATGTTTCTGATACGGGTATTCCAATTGATGTAACTCAAGTTGGGAAAGAAAT
AGTCGTTAATTTCCTGAATACCAAGATCCCTTTGAATTTAATGAAACGGTATGATGTTTCTGATTTTCACAGCCCTGTGC
AGATTATTACGATGCAACAGGAAGGCAAAAAGGTTCGCATGACCATCCTCAGCAAAGGGGATTATGGACATTTTGTTTAT
CAGGTCAATAAACAATTTATGGTCGATGTCTTTCCTTTAACTGCGGAAGAAATTCGTCAGGCGAAATTGAAGAAACAGGT
ATTTACTGGCAAACGTATTTCTTTAAATTTCCAGAATATTAGTATTCGAGCTGTACTTCAATTGCTGGCAGATTTTACTG
GAATCAATATGGTTGTCAGCGACAAAGTACAAGGAGATATTACGCTTAGATTAAATGATATCCCCTGGGATCAGGCTCTG
GATATCATTTTAACCACACAAGGGCTAGATAAGCGCAGAAAGGGCAACGTCATGTTGATAGATACCAAGGCCTCCATGGA
TAAAATGGAGGAAGAACAGCTCAAAAGTCAACAGACAATTGAAAAACTTGAGCCTATCCGTTCTGATTTAATACAAATTA
ATTATGCAAAGGCAGCAGATATTTCTGTTTTGATCAAGGATAAGCAAAACTCCCTATTATCAGACAGGGGAAAAATAAGT
GTTGATGCCAGAACGAATACAATCTGGATTCAGGATACTGGCACCAAAATTGAGGAAGTAAGGGAACTGATAAAGCAATT
GGATATCCCCGTTAAGCAAGTGCTTATTGAGGCAAGAATTGTTGAAGTGACTAAAGATTTTAGCCAGGATATTGGGATAC
GCTGGGGGGTATCAAGACCAACTCACTTAAGTGGTACACTGGAAGGTGCTAACGAGTTAGCGAGAGGCACAGCACCAGCC
AATGTTGTCCCCTTGGAAAGAAGATTAAATCTTGATTTGGCAGCCGCTCCTTTAACCGGAGCTACACCGGCCTCGGTAGG
TATTGCGCTAGCTAAACTTAATGATAACATTCTGCTTGATCTGGAATTGTCTGCTTTGGAAAGCGAAGGGCGTGCTGAAT
TAATATCCAGTCCTCGCTTGATTACTACGAATCAACAGGCAGCAGTCATAGAGTCTGGTGAGGAGATTCCCTATCAGGAG
GCAACATCAAGTGGAGCGACTGCTGTTGCATTTAAAAAAGCGGTATTGAGTTTGAAAGTTACCCCGCAAATTACGCCTGA
TGGTAAAATTTTGATGGATTTACAGATAAATCAGGATACTCCTTCCCCACAAACATTCCTTGGTGTTCCAGCAATAATAA
CAAAGGAGATTCAGACGAATGTTCTGGTTAATAATGGACAAACCATAGTACTTGGGGGTATTTATAAGCAAGATAAGAAC
AAGGTAATCAATAGAATTCCTTTCTTTGGTGAGTTGCCTGTTGTCGGTATTTTATTTAGTAATAGACAAATCACATTGAA
AAATGAAGAATTACTTATCTTTATTACACCAAGAATAATAACGAATGCCTTATCGATTACAACGATAGAGGGACGCGAGA
AAGATGTTTATAAATAA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(39-113)

Bacterial type II/III secretion system short domain (Secretin_N)

(370-433)

Bacterial type II and III secretion system protein (Secretin)

(525-680)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Legionella pneumophila strain Lp02

99.713

100

0.997
  pilQ Legionella pneumophila strain ERS1305867

99.57

100

0.996
  comQ Acinetobacter baylyi ADP1

38.574

98.424

0.38
  pilQ Pseudomonas aeruginosa PAK

37.589

100

0.38
  pilQ Acinetobacter baumannii strain A118

35.095

100

0.371
  pilQ Acinetobacter baumannii D1279779

35.095

100

0.371

Multiple sequence alignment