Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACJEHI_RS05745 Genome accession   NZ_CP174230
Coordinates   1290757..1292853 (-) Length   698 a.a.
NCBI ID   WP_010946666.1    Uniprot ID   Q5ZX02
Organism   Legionella pneumophila strain SPF634     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1285757..1297853
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJEHI_RS05725 (ACJEHI_05725) - 1286700..1287131 (-) 432 WP_010946670.1 universal stress protein -
  ACJEHI_RS05730 (ACJEHI_05730) - 1287240..1288691 (-) 1452 WP_010946669.1 SPOR domain-containing protein -
  ACJEHI_RS05735 (ACJEHI_05735) aroB 1288688..1289797 (-) 1110 WP_010946668.1 3-dehydroquinate synthase -
  ACJEHI_RS05740 (ACJEHI_05740) aroK 1289784..1290311 (-) 528 WP_010946667.1 shikimate kinase AroK -
  ACJEHI_RS05745 (ACJEHI_05745) pilQ 1290757..1292853 (-) 2097 WP_010946666.1 type IV pilus secretin PilQ Machinery gene
  ACJEHI_RS05750 (ACJEHI_05750) pilP 1292857..1293444 (-) 588 WP_010946665.1 pilus assembly protein PilP Machinery gene
  ACJEHI_RS05755 (ACJEHI_05755) pilO 1293441..1294040 (-) 600 WP_014841277.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACJEHI_RS05760 (ACJEHI_05760) pilN 1294054..1294602 (-) 549 WP_010946663.1 PilN domain-containing protein Machinery gene
  ACJEHI_RS05765 (ACJEHI_05765) pilM 1294606..1295670 (-) 1065 WP_010946662.1 type IV pilus assembly protein PilM Machinery gene
  ACJEHI_RS05770 (ACJEHI_05770) ravI 1296087..1297097 (+) 1011 WP_010946661.1 Dot/Icm T4SS effector RavI -

Sequence

Protein


Download         Length: 698 a.a.        Molecular weight: 77487.79 Da        Isoelectric Point: 9.5345

>NTDB_id=1075883 ACJEHI_RS05745 WP_010946666.1 1290757..1292853(-) (pilQ) [Legionella pneumophila strain SPF634]
MRKIIAILILISASLSMAYSKDNSLISVKVLPLPENRVRIDFQFSKPLKQLPASFITQKPPRLVLDFISTDMQIPSDQKT
KTIQLGSLHSYNIVAVGDRVRAILELDRSVSYSGSTAGSIYSLVLNGKSNDLFENSKEVFITNQVVNAKHEIKRIDFRGI
EKQGGRVLIDVSDTGIPIDVTQVGKEIVVNFLNTKIPLNLMKRYDVSDFHSPVQIITMQQEGKKVRMTILSKGDYGHFVY
QVNKQFMVDVFPLTAEEIRQAKLKKQVFTGKRISLNFQNISIRAVLQLLADFTGINMVVSDKVQGDITLRLNDIPWDQAL
DIILTTQGLDKRRKGNVMLIDTKASMDKMEEEQLKSQQTIEKLEPIRSDLIQINYAKAADIAVLIKDKQNSLLSDRGKIS
VDARTNTIWIQDTGTKIEEVRELIKQLDIPVKQVLIEARIVEVTKDFSQDIGIRWGVSRPTHLSGTLEGANELARGTAPA
NVVPLERRLNLDLAAAPLTGATPASVGIALAKLNDNILLDLELSALESEGRAELISSPRLITTNQQAAVIESGEEIPYQE
ATSSGATAVAFKKAVLSLKVTPQITPDGKILMDLQINQDTPSPQTFLGVPAIITKEIQTNVLVNNGQTIVLGGIYKQDKN
KVINRIPFFGELPVVGILFSNRQITLKNEELLIFITPRIITNALSITTIEGREKDVYK

Nucleotide


Download         Length: 2097 bp        

>NTDB_id=1075883 ACJEHI_RS05745 WP_010946666.1 1290757..1292853(-) (pilQ) [Legionella pneumophila strain SPF634]
GTGAGAAAAATTATCGCGATTTTGATATTAATTAGTGCCAGTTTGAGTATGGCATATTCTAAAGATAATTCGCTCATTTC
AGTAAAAGTATTGCCATTGCCCGAAAACAGAGTGCGTATTGATTTTCAATTTTCCAAGCCCTTAAAGCAGTTGCCTGCAA
GCTTTATTACTCAAAAGCCGCCCCGTTTGGTGTTAGATTTTATCAGCACTGATATGCAAATTCCTTCGGATCAGAAAACT
AAAACTATTCAATTAGGCTCATTACACAGTTATAACATTGTTGCTGTTGGTGATAGAGTAAGAGCCATATTGGAACTGGA
TCGTTCAGTATCCTATTCTGGCAGCACGGCGGGTTCCATTTATAGCCTTGTCCTGAATGGTAAAAGCAATGATTTATTTG
AAAATAGCAAGGAGGTATTCATAACGAACCAGGTTGTGAACGCCAAGCACGAAATTAAAAGAATTGATTTTCGTGGTATA
GAAAAGCAGGGTGGTAGAGTCTTAATTGACGTTTCTGATACGGGTATTCCAATTGATGTAACTCAAGTTGGGAAAGAAAT
AGTCGTTAATTTCCTGAATACCAAGATCCCTTTGAATTTAATGAAACGGTATGATGTTTCTGATTTTCACAGCCCTGTGC
AGATTATTACGATGCAACAGGAAGGCAAGAAGGTTCGCATGACCATTCTCAGCAAAGGGGATTATGGACATTTTGTGTAT
CAGGTCAATAAACAATTTATGGTTGATGTCTTTCCTTTAACTGCGGAAGAAATTCGTCAGGCGAAATTAAAGAAACAGGT
ATTTACCGGTAAACGTATTTCTTTGAATTTCCAGAATATTAGTATTCGGGCTGTACTTCAATTGCTGGCAGATTTTACTG
GAATCAATATGGTTGTCAGCGACAAAGTACAAGGAGATATTACGCTTAGATTAAATGATATCCCCTGGGATCAGGCTCTG
GATATCATTTTAACCACACAAGGGCTAGATAAGCGCAGAAAAGGCAACGTCATGTTGATAGATACCAAGGCCTCCATGGA
TAAAATGGAGGAAGAACAACTCAAAAGTCAACAGACAATTGAAAAACTTGAGCCTATCCGTTCTGATTTAATACAGATTA
ATTATGCAAAGGCAGCAGATATTGCTGTTTTGATCAAGGATAAGCAAAATTCCCTATTATCAGACAGAGGAAAAATAAGT
GTTGATGCCAGAACGAATACAATCTGGATTCAGGATACTGGCACCAAAATTGAGGAAGTAAGGGAACTGATAAAGCAATT
GGATATCCCTGTTAAGCAAGTGCTTATTGAGGCAAGAATTGTTGAAGTAACTAAAGATTTTAGCCAGGATATTGGGATAC
GCTGGGGGGTATCAAGACCAACCCACTTAAGTGGTACACTGGAAGGTGCTAACGAGTTAGCGAGAGGCACAGCACCAGCC
AATGTTGTCCCCTTGGAAAGAAGGTTAAATCTTGATTTGGCAGCTGCTCCTTTAACTGGAGCTACACCAGCCTCGGTAGG
TATTGCGCTAGCTAAACTTAATGATAACATTCTGCTTGATCTGGAATTGTCTGCTTTGGAAAGCGAAGGGCGTGCTGAAT
TAATATCCAGTCCTCGCCTGATTACTACGAATCAACAGGCAGCAGTCATAGAGTCTGGTGAGGAGATACCCTATCAGGAG
GCAACATCAAGTGGAGCGACTGCTGTTGCATTTAAAAAAGCGGTATTGAGTTTGAAAGTTACCCCACAAATTACGCCTGA
TGGTAAAATTTTGATGGATTTACAGATAAATCAGGATACTCCTTCCCCACAAACATTCCTTGGTGTTCCAGCAATAATAA
CAAAGGAGATTCAGACGAATGTTCTGGTTAATAATGGACAAACCATAGTACTTGGGGGTATTTATAAGCAAGATAAGAAC
AAGGTAATCAATAGAATTCCTTTCTTTGGTGAGTTGCCTGTTGTCGGTATTTTATTTAGTAATAGACAAATCACATTGAA
AAATGAGGAATTACTGATTTTTATTACTCCAAGAATAATAACGAATGCCTTATCGATTACAACGATAGAGGGACGTGAAA
AGGATGTTTATAAATAA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(39-113)

Bacterial type II/III secretion system short domain (Secretin_N)

(370-433)

Bacterial type II and III secretion system protein (Secretin)

(525-680)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5ZX02

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Legionella pneumophila strain Lp02

100

100

1
  pilQ Legionella pneumophila strain ERS1305867

99.57

100

0.996
  pilQ Pseudomonas aeruginosa PAK

37.73

100

0.381
  comQ Acinetobacter baylyi ADP1

38.428

98.424

0.378
  pilQ Acinetobacter baumannii strain A118

35.095

100

0.371
  pilQ Acinetobacter baumannii D1279779

35.095

100

0.371

Multiple sequence alignment