Detailed information NTG1074928

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACJA24_RS12225
• Genome accession: NZ_CP174198
• Coordinates: 2518177..2518791 (-)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain LQ-03
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2513177..2523791

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACJA24_RS12195	rpsT	2513477..2513743 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACJA24_RS12200	holA	2513760..2514801 (-)	1042	Protein_2355	DNA polymerase III subunit delta	-
ACJA24_RS12205	-	2514841..2514995 (-)	155	Protein_2356	hypothetical protein	-
ACJA24_RS12210	-	2515036..2515170 (+)	135	WP_003152870.1	YqzM family protein	-
ACJA24_RS12215	comEC	2515189..2517540 (-)	2352	WP_041481887.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACJA24_RS12220	-	2517541..2518110 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACJA24_RS12225	comEA	2518177..2518791 (-)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACJA24_RS12230	comER	2518850..2519671 (+)	822	WP_014305449.1	late competence protein ComER	-
ACJA24_RS12235	-	2519740..2520477 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
ACJA24_RS12240	rsfS	2520474..2520830 (-)	357	WP_014305451.1	ribosome silencing factor	-
ACJA24_RS12245	yqeK	2520827..2521408 (-)	582	WP_014305452.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACJA24_RS12250	-	2521398..2521967 (-)	570	WP_014305453.1	nicotinate-nucleotide adenylyltransferase	-
ACJA24_RS12255	yhbY	2521978..2522268 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ACJA24_RS12260	aroE	2522262..2523104 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=1074928 ACJA24_RS12225 WP_003152867.1 2518177..2518791(-) (comEA) [Bacillus velezensis strain LQ-03]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1074928 ACJA24_RS12225 WP_003152867.1 2518177..2518791(-) (comEA) [Bacillus velezensis strain LQ-03]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363