Detailed information NTG1074768

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACCO34_RS12500
• Genome accession: NZ_CP174160
• Coordinates: 2547310..2547924 (-)
• Length: 204 a.a.
• NCBI ID: WP_043867297.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain SMT-24
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2542310..2552924

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACCO34_RS12470	rpsT	2542610..2542876 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACCO34_RS12475	holA	2542893..2543934 (-)	1042	Protein_2410	DNA polymerase III subunit delta	-
ACCO34_RS12480	-	2543974..2544128 (-)	155	Protein_2411	hypothetical protein	-
ACCO34_RS12485	-	2544169..2544303 (+)	135	WP_003152870.1	YqzM family protein	-
ACCO34_RS12490	comEC	2544322..2546673 (-)	2352	WP_139890043.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACCO34_RS12495	-	2546674..2547243 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACCO34_RS12500	comEA	2547310..2547924 (-)	615	WP_043867297.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACCO34_RS12505	comER	2547983..2548804 (+)	822	WP_139890042.1	late competence protein ComER	-
ACCO34_RS12510	-	2548873..2549610 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
ACCO34_RS12515	rsfS	2549607..2549963 (-)	357	WP_003152864.1	ribosome silencing factor	-
ACCO34_RS12520	yqeK	2549960..2550541 (-)	582	WP_057080474.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACCO34_RS12525	-	2550531..2551100 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
ACCO34_RS12530	yhbY	2551111..2551401 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ACCO34_RS12535	aroE	2551395..2552237 (-)	843	WP_025852995.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22178.37 Da
• Isoelectric Point: 6.9685

>NTDB_id=1074768 ACCO34_RS12500 WP_043867297.1 2547310..2547924(-) (comEA) [Bacillus velezensis strain SMT-24]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEGNHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1074768 ACCO34_RS12500 WP_043867297.1 2547310..2547924(-) (comEA) [Bacillus velezensis strain SMT-24]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGGGAATCATCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGAAGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363