Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACI514_RS08550 Genome accession   NZ_CP173611
Coordinates   1878215..1879324 (+) Length   369 a.a.
NCBI ID   WP_160058238.1    Uniprot ID   A0A7Z2KVW2
Organism   Pseudomonas sp. M20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1873215..1884324
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI514_RS08535 (ACI514_08535) - 1874191..1875123 (+) 933 WP_160058242.1 sugar kinase -
  ACI514_RS08540 (ACI514_08540) - 1875237..1875512 (-) 276 WP_007912322.1 peptidylprolyl isomerase -
  ACI514_RS08545 (ACI514_08545) - 1875564..1878101 (-) 2538 WP_405123542.1 PAS domain S-box protein -
  ACI514_RS08550 (ACI514_08550) pilU 1878215..1879324 (+) 1110 WP_160058238.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACI514_RS08555 (ACI514_08555) - 1879461..1883234 (+) 3774 WP_160058236.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40731.68 Da        Isoelectric Point: 7.6533

>NTDB_id=1073152 ACI514_RS08550 WP_160058238.1 1878215..1879324(+) (pilU) [Pseudomonas sp. M20]
MEIDALLSILSNKNGSDLFLSTGAPPSARFDGVLTALSERPFKNGETAAIAASLMDAEQRREFDRDLEMNLAISRAGIGR
FRVNIFKQRNDISIVIRNVKLDIPRFADLKLPAVLLDTVMLKQGLILFVGATDSGKSTSLAALIDHRNRHSSGHIITIED
PIEYIHRHQRSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVLSTLHATNANKALERI
INMFAEERRPQLLQVLGNNLRAFISQRLVRTVDGQRRAAVEVLLGTPTIADLVRRGQFEELKPIMEKSTGLGMQTFDGAL
FALVAEGVISAQEALKNADSVNNLKLRMKLSGEESSSRGSTPEGWGLMD

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1073152 ACI514_RS08550 WP_160058238.1 1878215..1879324(+) (pilU) [Pseudomonas sp. M20]
ATGGAAATCGATGCACTGTTGTCAATTTTGTCCAATAAAAATGGTTCGGACTTGTTCCTTTCCACGGGTGCTCCGCCCAG
TGCGCGTTTCGACGGCGTGCTCACGGCGCTGAGCGAGCGTCCTTTCAAGAACGGTGAGACCGCCGCCATCGCCGCCTCCC
TGATGGACGCCGAGCAGCGGCGGGAGTTCGACCGGGATCTGGAAATGAACCTGGCGATTTCGCGAGCGGGCATTGGTCGC
TTTCGCGTAAACATCTTCAAGCAGCGCAACGATATATCGATCGTCATTCGTAACGTCAAACTCGACATCCCGCGTTTCGC
CGATCTGAAGCTGCCAGCGGTACTGCTCGACACTGTCATGCTCAAGCAAGGGTTGATTCTGTTTGTTGGCGCCACCGACT
CGGGGAAATCAACCTCGCTCGCCGCGCTGATCGATCACCGCAACCGCCACAGCAGCGGGCACATCATCACCATCGAGGAT
CCGATCGAGTACATCCATCGGCACCAGCGTTCGATCATCAATCAACGTGAAGTCGGGGTCGATACGCGCAGCTTTCATGC
GGCGTTGAAGAACACTTTGCGCCAGGCGCCGGATGTGGTGCTGATCGGTGAAATCCGTGATCGCGAAACCATGGAACATG
CTTTGGCGTTTGCCGATACCGGGCATCTTGTGTTGTCGACGTTGCATGCCACGAATGCCAACAAGGCGTTGGAGCGGATT
ATCAATATGTTTGCGGAAGAGCGGCGGCCGCAGTTGTTGCAGGTGCTGGGCAATAATCTGAGAGCCTTTATTTCGCAGCG
CTTGGTGCGCACTGTCGATGGGCAACGAAGGGCCGCAGTGGAGGTGCTGTTGGGTACGCCAACCATCGCCGATCTGGTTC
GGCGCGGTCAGTTCGAGGAACTGAAACCAATAATGGAAAAATCCACCGGTCTGGGTATGCAGACGTTTGACGGAGCCCTG
TTCGCATTGGTCGCTGAAGGTGTAATCAGTGCGCAGGAAGCTTTGAAGAATGCAGATTCGGTTAATAACTTGAAGTTGCG
GATGAAACTGTCCGGAGAGGAAAGTAGTAGCCGTGGTTCAACGCCAGAAGGCTGGGGATTAATGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(57-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z2KVW2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.085

95.935

0.528
  pilU Acinetobacter baylyi ADP1

52.958

96.206

0.509
  pilU Vibrio cholerae strain A1552

49.307

97.832

0.482
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.541

90.244

0.366
  pilT Vibrio cholerae strain A1552

40.541

90.244

0.366
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.528

91.87

0.363

Multiple sequence alignment