Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACI3KK_RS20535 Genome accession   NZ_CP173421
Coordinates   4290686..4292770 (-) Length   694 a.a.
NCBI ID   WP_405121185.1    Uniprot ID   -
Organism   Pseudomonas leptonychotis strain TMP18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4285686..4297770
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI3KK_RS20520 (ACI3KK_20530) - 4287423..4288997 (-) 1575 WP_405119174.1 SPOR domain-containing protein -
  ACI3KK_RS20525 (ACI3KK_20535) aroB 4289008..4290111 (-) 1104 WP_405121184.1 3-dehydroquinate synthase -
  ACI3KK_RS20530 (ACI3KK_20540) aroK 4290163..4290681 (-) 519 WP_136663369.1 shikimate kinase AroK -
  ACI3KK_RS20535 (ACI3KK_20545) pilQ 4290686..4292770 (-) 2085 WP_405121185.1 type IV pilus secretin PilQ Machinery gene
  ACI3KK_RS20540 (ACI3KK_20550) pilP 4292826..4293347 (-) 522 WP_405119175.1 type 4a pilus biogenesis lipoprotein PilP -
  ACI3KK_RS20545 (ACI3KK_20555) pilO 4293347..4293967 (-) 621 WP_136663371.1 type 4a pilus biogenesis protein PilO -
  ACI3KK_RS20550 (ACI3KK_20560) - 4293967..4294536 (-) 570 WP_405119176.1 PilN domain-containing protein -
  ACI3KK_RS20555 (ACI3KK_20565) pilM 4294536..4295600 (-) 1065 WP_136663373.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 694 a.a.        Molecular weight: 74665.67 Da        Isoelectric Point: 4.8732

>NTDB_id=1072195 ACI3KK_RS20535 WP_405121185.1 4290686..4292770(-) (pilQ) [Pseudomonas leptonychotis strain TMP18]
MNMSPSFLAVTLLATFLSPFASAADLNAIDVAALPGDRVELKLTFDEPVLAPRGYTIEQPARIALDLPGVSNKLGTKNRQ
LGIGNARSVTVVEAKDRTRLVINLTSLSPYYTRAEGNVLFVVVGEGAKEVSSAAPSVANTVAPAKKYAPKARSINDIDFQ
RGEQGEGNIVITLSDASVSPDIQDQGGKIRLDFSKTQLPDTLRVKLDVKDFATPVQYVSTTGTADKVSVVIESSGIYDYL
AYQTDNKLTISVKPLTQVEAEKRKADSFAYAGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVAGNITLRLQNVPWDQ
ALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELIQVNYAKASDMARLFQSVTSADGTTDSR
GSVTVDDRTNSIIAYQTQERLDELRRIVAQLDVAVRQVMIEARIVEANVDYDKSLGVNWRGASVGDSNFVVGGSGLDRPG
APGYGQNAGIRNPGSVLGNFVDLGATNATSGLGIGFITDNAILDLELSAMEKTGNGEVVSQPKVVTSDKETAKILKGSEI
PYQEASSSGASTTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPDFGRAVNGVPPINKNEVNAKVLVSDGETIVIGGVF
SNTQTKSVDKVPFLGDLPYLGRMFKRDIVSDNKSELLIFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2085 bp        

>NTDB_id=1072195 ACI3KK_RS20535 WP_405121185.1 4290686..4292770(-) (pilQ) [Pseudomonas leptonychotis strain TMP18]
ATGAATATGTCGCCCTCTTTTTTAGCGGTTACTCTCCTGGCTACGTTTCTCTCGCCATTCGCTTCAGCTGCAGACCTAAA
TGCAATTGACGTTGCGGCTCTGCCAGGTGACCGGGTTGAGTTAAAGCTAACATTTGATGAGCCTGTGCTTGCGCCTAGAG
GTTATACCATTGAGCAGCCGGCACGTATTGCTCTCGATCTGCCAGGAGTGTCGAATAAGTTAGGTACAAAGAATCGACAG
CTTGGTATTGGCAATGCGCGCAGCGTTACAGTCGTTGAGGCCAAAGATCGCACGCGTCTCGTAATCAATTTGACTAGCCT
CTCTCCGTACTACACTCGTGCAGAGGGCAACGTTCTTTTTGTAGTGGTTGGCGAGGGTGCTAAGGAAGTTTCTTCTGCTG
CGCCTTCTGTCGCCAATACCGTGGCACCTGCGAAAAAGTATGCACCTAAAGCCAGATCGATTAATGATATAGATTTTCAG
CGAGGAGAGCAGGGTGAAGGTAATATTGTTATTACTTTGTCTGACGCCTCGGTAAGTCCGGACATACAAGACCAGGGTGG
GAAAATCCGACTGGATTTTTCTAAAACACAGCTGCCTGACACACTGCGTGTAAAGCTTGATGTGAAAGATTTTGCTACTC
CAGTTCAGTATGTGAGTACGACTGGAACTGCTGATAAAGTGAGTGTTGTTATTGAGTCGTCAGGTATTTATGACTATCTG
GCATATCAAACAGACAATAAGTTAACCATAAGCGTGAAGCCACTTACTCAGGTGGAGGCTGAAAAGCGTAAGGCTGATAG
CTTTGCCTATGCGGGCGAGAAGTTGTCTCTTAATTTTCAAGATATTGATGTTCGTTCGGTTCTGCAGTTAATAGCAGATT
TTACCGACCTTAATTTAGTTGCTAGCGATACTGTGGCAGGTAATATTACGCTTCGGTTGCAGAACGTTCCTTGGGACCAA
GCCTTGGACTTGGTTTTAAAAACCAAGGGTCTAGATAAGCGAAAAATTGGAAATGTACTTCTTGTGGCTCCTGCAGATGA
GATAGCGGCGCGCGAACGCCAAGAGCTTGAGTCGCAGAAACAAATTGCTGAGTTAGCCCCATTGCGACGTGAGCTTATTC
AGGTGAATTATGCGAAGGCATCTGATATGGCTCGGCTTTTTCAGTCTGTTACCAGTGCGGATGGCACTACAGACTCCCGT
GGCTCAGTTACTGTTGACGATAGAACAAACAGTATCATTGCGTACCAGACCCAAGAGCGTCTTGATGAGTTGCGGCGAAT
TGTGGCGCAGCTGGATGTCGCGGTTCGACAAGTAATGATCGAGGCTAGAATTGTTGAAGCTAATGTCGATTATGATAAAT
CGCTTGGGGTTAATTGGCGTGGTGCCAGCGTTGGGGATTCTAATTTTGTTGTTGGTGGGAGTGGTTTGGATCGCCCTGGT
GCGCCGGGATATGGGCAGAACGCGGGCATTCGAAATCCGGGATCAGTTCTTGGTAATTTTGTAGATCTTGGTGCCACGAA
TGCAACTTCTGGCCTGGGAATTGGTTTCATTACTGATAATGCAATTTTGGACCTTGAGCTTTCTGCAATGGAAAAGACGG
GTAATGGTGAGGTCGTATCACAGCCTAAAGTTGTCACGTCGGACAAAGAAACAGCTAAAATTCTTAAAGGTTCAGAAATA
CCCTATCAAGAGGCCAGCTCGAGCGGAGCGAGCACAACTTCGTTTAAAGAAGCTGCCTTGTCTCTTGAGGTTACTCCCCA
AATAACGCCTGATAATCGTATTATTATGGAAGTCAAGGTTACTAAAGATGCGCCTGATTTTGGGCGGGCTGTAAACGGTG
TCCCTCCGATTAACAAGAATGAAGTAAATGCGAAGGTTCTTGTTAGTGATGGCGAGACTATAGTTATCGGCGGTGTTTTT
TCTAATACACAAACCAAGAGCGTCGATAAGGTGCCTTTCTTGGGTGACTTGCCCTACTTAGGCCGTATGTTCAAACGTGA
TATTGTGTCAGATAATAAATCTGAGCTTTTGATATTTCTAACGCCTCGTATCATGAACAATCAAGCTATCGCGGTGAGTC
GTTGA

Domains

Predicted by InterProScan.

AMIN domain (AMIN)

(38-116)

Secretin and TonB N terminus short domain (STN)

(298-344)

Bacterial type II and III secretion system protein (Secretin)

(529-685)

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.786

100

0.767
  comQ Acinetobacter baylyi ADP1

42.63

99.712

0.425
  pilQ Acinetobacter baumannii strain A118

40.942

100

0.414
  pilQ Acinetobacter baumannii D1279779

40.942

100

0.414
  pilQ Legionella pneumophila strain ERS1305867

36.652

99.856

0.366
  pilQ Legionella pneumophila strain Lp02

36.508

99.856

0.365

Multiple sequence alignment