Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACIO08_RS09710 Genome accession   NZ_CP173233
Coordinates   1977881..1979305 (+) Length   474 a.a.
NCBI ID   WP_035687022.1    Uniprot ID   -
Organism   Avibacterium paragallinarum strain npAP/OH-USA/20230213/S1-2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1972881..1984305
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIO08_RS09680 (ACIO08_09680) lgt 1973456..1974256 (-) 801 WP_264256694.1 prolipoprotein diacylglyceryl transferase -
  ACIO08_RS09685 (ACIO08_09685) - 1974266..1975057 (-) 792 WP_264256695.1 sulfite exporter TauE/SafE family protein -
  ACIO08_RS09690 (ACIO08_09690) rppH 1975060..1975653 (-) 594 WP_264245767.1 RNA pyrophosphohydrolase -
  ACIO08_RS09695 (ACIO08_09695) - 1976225..1976644 (-) 420 WP_264256696.1 hypothetical protein -
  ACIO08_RS09700 (ACIO08_09700) ampD 1976743..1977297 (-) 555 WP_264245770.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACIO08_RS09705 (ACIO08_09705) pilA 1977437..1977868 (+) 432 WP_035687024.1 pilin Machinery gene
  ACIO08_RS09710 (ACIO08_09710) pilB 1977881..1979305 (+) 1425 WP_035687022.1 GspE/PulE family protein Machinery gene
  ACIO08_RS09715 (ACIO08_09715) pyrH 1979555..1980271 (+) 717 WP_035687020.1 UMP kinase -
  ACIO08_RS09720 (ACIO08_09720) frr 1980296..1980853 (+) 558 WP_017807054.1 ribosome recycling factor -
  ACIO08_RS09725 (ACIO08_09725) ispC 1981035..1982231 (+) 1197 WP_110479821.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
  ACIO08_RS09730 (ACIO08_09730) uppS 1982385..1983113 (+) 729 WP_264256698.1 polyprenyl diphosphate synthase -
  ACIO08_RS09735 (ACIO08_09735) - 1983117..1983980 (+) 864 WP_264256699.1 phosphatidate cytidylyltransferase -

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 53762.51 Da        Isoelectric Point: 5.2146

>NTDB_id=1071054 ACIO08_RS09710 WP_035687022.1 1977881..1979305(+) (pilB) [Avibacterium paragallinarum strain npAP/OH-USA/20230213/S1-2]
MAEYSLDEDDIVYAVDGKCFEITPHLWQQNKQQKDILIRYLAVPLQEDEHTLWLGIDDLTNLSACESFSFLTGKIVEPIL
LSEQDLKSLLQKLSPTLVEEPALLDFYQSSISPYSENQPEKLDEPIIQILNKVFESAMQLNASDIHLEPQNEQLQIRFRI
DGVLQHQPPISKQMSQRLISRLKLLARLDLSETRLPQDGRFHFKTTFSDILDFRISTLPTYYGEKAVLRLQKNKPVTLDF
SELGMTAIQRKTLQDALSQPQGLILVTGPTGSGKSITLYTALEWLNQSEKHILTAEDPIEIELPGIIQTQINPQIGLDFN
RLLRTFLRQDPDIIMLGEIRDAESAAIALRAAQTGHLVLSTLHTNNAHSAIARLLQLGIQQYEIESCLLLVIAQRLVRKK
CLHKTKNCECYQGYSGRTGIYQFLQAIFDSNTGQISYQTDYPSLWDSASHKIAQGITDIAELERVLGRHTKAEL

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=1071054 ACIO08_RS09710 WP_035687022.1 1977881..1979305(+) (pilB) [Avibacterium paragallinarum strain npAP/OH-USA/20230213/S1-2]
ATGGCTGAGTATTCTTTAGACGAGGACGACATTGTATATGCTGTTGATGGAAAATGCTTTGAAATAACACCGCACTTATG
GCAACAAAATAAGCAGCAAAAAGATATTTTAATACGTTATTTAGCAGTACCTTTACAGGAGGATGAACATACACTTTGGC
TTGGTATTGATGACTTAACAAATCTATCGGCTTGTGAAAGTTTTTCATTTCTAACAGGAAAAATAGTTGAGCCGATTTTG
TTATCAGAACAAGATTTAAAATCGCTATTACAGAAGTTATCTCCCACATTAGTAGAAGAGCCGGCATTACTTGATTTTTA
TCAATCGTCTATTTCACCTTATAGCGAAAATCAACCAGAAAAACTTGATGAACCAATTATTCAAATACTAAATAAAGTTT
TTGAATCGGCAATGCAATTAAATGCCTCAGATATTCATTTAGAGCCACAGAATGAGCAATTGCAGATCCGTTTTCGTATT
GACGGGGTATTGCAACATCAGCCTCCTATTTCTAAACAAATGAGCCAACGTTTGATTTCTCGTTTAAAACTTCTAGCTCG
TCTTGATCTTAGCGAAACTCGCTTACCACAAGATGGAAGATTTCATTTCAAAACGACATTTTCCGATATATTAGACTTCC
GTATCTCGACATTACCTACATATTATGGAGAAAAAGCGGTACTACGTTTACAAAAAAATAAACCTGTTACCTTGGATTTT
TCTGAGCTGGGGATGACAGCGATACAACGAAAAACATTACAAGATGCTTTATCTCAACCTCAAGGACTCATTTTAGTAAC
GGGGCCAACCGGTAGTGGAAAAAGTATTACTCTCTATACAGCATTAGAATGGCTTAATCAATCGGAAAAACATATTCTAA
CAGCAGAAGATCCGATTGAAATAGAACTTCCAGGGATTATCCAAACGCAAATTAACCCACAAATTGGTTTAGATTTTAAC
CGTTTGTTGCGTACATTTTTACGTCAGGATCCAGACATTATTATGCTTGGTGAGATCCGTGATGCAGAAAGTGCAGCGAT
TGCGTTACGTGCAGCACAAACTGGACATTTAGTCCTTTCAACATTACATACAAATAATGCACATTCAGCTATTGCTCGCT
TGTTACAGCTTGGTATTCAGCAATATGAAATCGAAAGTTGTTTATTATTAGTGATTGCCCAGCGTTTAGTCCGTAAAAAA
TGTCTACACAAAACGAAAAATTGCGAATGTTATCAAGGTTATAGTGGGCGTACTGGTATTTATCAATTTTTACAAGCTAT
TTTTGATTCAAATACAGGGCAAATTTCTTATCAAACCGATTATCCCTCATTATGGGATAGTGCTTCACATAAAATAGCAC
AAGGTATCACAGATATTGCTGAATTAGAACGAGTTTTAGGTAGGCATACAAAAGCTGAACTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(132-399)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-95)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae Rd KW20

64.693

99.789

0.646
  pilB Haemophilus influenzae 86-028NP

63.924

100

0.639
  pilB Glaesserella parasuis strain SC1401

57.263

100

0.574
  pilB Legionella pneumophila strain ERS1305867

39.634

100

0.411
  pilB Vibrio campbellii strain DS40M4

40.206

100

0.411
  pilB Vibrio parahaemolyticus RIMD 2210633

39.794

100

0.407
  pilB Vibrio cholerae strain A1552

39.419

100

0.401
  pilB Acinetobacter baylyi ADP1

37.626

100

0.395
  pilF Neisseria gonorrhoeae MS11

37.317

100

0.376
  pilB Acinetobacter baumannii D1279779

36.495

100

0.373

Multiple sequence alignment