Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACFELB_RS24380 Genome accession   NZ_CP172850
Coordinates   5241756..5243456 (+) Length   566 a.a.
NCBI ID   WP_058355294.1    Uniprot ID   A0A643J963
Organism   Pseudomonas aeruginosa strain 238638E_ID017     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5236756..5248456
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFELB_RS24355 (ACFELB_24355) nadC 5237236..5238084 (+) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACFELB_RS24370 (ACFELB_24370) - 5239590..5240984 (-) 1395 WP_400589685.1 O-antigen ligase family protein -
  ACFELB_RS24375 (ACFELB_24375) - 5241058..5241525 (-) 468 WP_074227024.1 pilin -
  ACFELB_RS24380 (ACFELB_24380) pilB 5241756..5243456 (+) 1701 WP_058355294.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACFELB_RS24385 (ACFELB_24385) pilC 5243460..5244677 (+) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  ACFELB_RS24390 (ACFELB_24390) pilD 5244678..5245550 (+) 873 WP_003107301.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  ACFELB_RS24395 (ACFELB_24395) coaE 5245547..5246158 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  ACFELB_RS24400 (ACFELB_24400) yacG 5246155..5246355 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  ACFELB_RS24405 (ACFELB_24405) - 5246392..5246601 (-) 210 WP_003094660.1 hypothetical protein -
  ACFELB_RS24410 (ACFELB_24410) - 5246707..5247396 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  ACFELB_RS24415 (ACFELB_24415) - 5247393..5247863 (-) 471 WP_003094664.1 hypothetical protein -
  ACFELB_RS24420 (ACFELB_24420) - 5247860..5248285 (-) 426 WP_016562341.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62356.26 Da        Isoelectric Point: 5.4489

>NTDB_id=1069212 ACFELB_RS24380 WP_058355294.1 5241756..5243456(+) (pilB) [Pseudomonas aeruginosa strain 238638E_ID017]
MNDSTPLSGLAKQLVLNELLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
VISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQAVTDIQFSTGLTTEALLVEDDKLGVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAESASQEDSGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKNKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
ELIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1069212 ACFELB_RS24380 WP_058355294.1 5241756..5243456(+) (pilB) [Pseudomonas aeruginosa strain 238638E_ID017]
ATGAACGATTCCACGCCTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACGAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTGCAGAGCCGGGCGT
TGGTCGAGGTAGCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GTCATCAGCGAGCGCCTGGTCCGCCAGCACCGCGTGTTGCCGCTGTGGAAACGCGGCAACAAACTGTTCATCGGCCTCTC
CGACCCAACCAATCACCAAGCGGTCACCGATATCCAGTTCAGCACCGGCCTCACCACCGAGGCCCTGCTGGTGGAGGACG
ACAAGCTCGGCGTAGCCATCGAGAAGCTCTACGAGGGCGCCATGGATGCCCTCGGCGATCTCGGCGACATCGATCTCGAT
GGAGTCGATGTGGGCAAGGCAGAAAGCGCCAGCCAGGAAGATTCCGGCGACGACGCCGAAGATGCCCCGGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGCTCTTCCGACCTGCACTTCGAACCCTACGAAAAGACCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAGGTAGCCAAGCCGCCGATCCAGCTGGCCAGCCGCATTTCCGCGCGACTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGGCGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCAAAGAACAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCCCTCGGCTACGAAGAGGATCAGAAGGAACTTTATCTGGCCGCGCTCAAACAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTTTCGCTGTACACCGGCCTGAATATCCTCAATACCACCGA
CATCAATATCTCCACCGCCGAAGACCCTGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGGCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAAGGTTTCCCCGAG
GAGCTGATCGGCACCTTCAAGCTGTATTCGCCTGTAGGCTGCGAAAACTGCAAAGGCGGTTACAAAGGTCGCGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A643J963

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.719

100

0.581
  pilB Acinetobacter baylyi ADP1

56.614

100

0.567
  pilB Legionella pneumophila strain ERS1305867

54.641

100

0.551
  pilB Vibrio cholerae strain A1552

51.699

98.763

0.511
  pilF Neisseria gonorrhoeae MS11

50.355

99.647

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

52.345

94.17

0.493
  pilB Vibrio campbellii strain DS40M4

51.407

94.17

0.484
  pilB/pilB1 Synechocystis sp. PCC 6803

37.763

100

0.412
  pilF Thermus thermophilus HB27

38.84

100

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.909

97.173

0.378

Multiple sequence alignment