Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACG1BZ_RS17885 Genome accession   NZ_CP172449
Coordinates   4025637..4027349 (+) Length   570 a.a.
NCBI ID   WP_413663000.1    Uniprot ID   -
Organism   Microbulbifer sp. CNSA002     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4020637..4032349
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACG1BZ_RS17875 (ACG1BZ_17875) - 4023882..4024628 (-) 747 WP_413662998.1 hypothetical protein -
  ACG1BZ_RS17880 (ACG1BZ_17880) - 4024694..4025251 (-) 558 WP_413662999.1 pilin -
  ACG1BZ_RS17885 (ACG1BZ_17885) pilB 4025637..4027349 (+) 1713 WP_413663000.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACG1BZ_RS17890 (ACG1BZ_17890) pilC 4027506..4028720 (+) 1215 WP_413663001.1 type II secretion system F family protein Machinery gene
  ACG1BZ_RS17895 (ACG1BZ_17895) - 4028726..4029610 (+) 885 WP_413663002.1 prepilin peptidase -
  ACG1BZ_RS17900 (ACG1BZ_17900) coaE 4029721..4030329 (+) 609 WP_413663003.1 dephospho-CoA kinase -
  ACG1BZ_RS17905 (ACG1BZ_17905) yacG 4030649..4030864 (+) 216 WP_108734183.1 DNA gyrase inhibitor YacG -
  ACG1BZ_RS17910 (ACG1BZ_17910) mutT 4030918..4031316 (-) 399 WP_413663004.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62362.10 Da        Isoelectric Point: 4.8582

>NTDB_id=1067901 ACG1BZ_RS17885 WP_413663000.1 4025637..4027349(+) (pilB) [Microbulbifer sp. CNSA002]
MTAPPLSGLAKRLVADQIFDESTALSAVKAARRENQSFVQHIAETKLVKSSELAKIASQAFGTPLFDLSSYNFELIPRDI
VDDKLVSKHFALPLYKRGNRLFVAVADPTNLAGLDEINFNTGLNTDPVIVEADKLAKAIENYLSQGDDMGAGLDGMEDEE
LDSLNVGEDDSDRGNDEEPGGDEAPVVRFVNKVLLDAIRTGASDIHFEPYEKSYRVRFRTDGVLHEIAKPPIQLASRLSA
RLKVMSKMDISEKRVPQDGRIKMKLSKTKAIDFRVNSLPTLWGEKIVLRILDPSSAKLGIDALGYEDEQKQIYMDALAQP
QGMILVTGPTGSGKTVSLYTGLNILNTPELNISTAEDPVEINLEGINQVNVSAKVGLDFAEALRSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLMNMGVPTFNIATSVSVIIAQRLARRLCNECKKPTTLPPEVLEAEGF
SAVTIPESDWSIFQPVGCEHCSKGYKGRVGVYEVVRITDGISRIIMEGGNSIQIADQARQEGFNNLRVSALRKVVMGVTS
LEEANRVTKD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1067901 ACG1BZ_RS17885 WP_413663000.1 4025637..4027349(+) (pilB) [Microbulbifer sp. CNSA002]
ATGACAGCACCCCCTCTTAGCGGCCTAGCCAAGCGTTTGGTTGCCGATCAAATTTTTGATGAATCAACCGCGCTATCTGC
CGTTAAAGCGGCCCGGCGAGAAAATCAAAGCTTTGTCCAGCATATAGCGGAAACCAAACTGGTTAAAAGCTCTGAGCTCG
CCAAGATCGCTTCACAGGCATTTGGCACTCCGCTTTTTGACCTCAGCAGCTATAACTTTGAGCTGATTCCAAGGGATATC
GTCGACGATAAGCTTGTCAGCAAACACTTCGCTCTTCCACTGTATAAAAGGGGCAACCGGCTATTTGTAGCGGTGGCGGA
CCCAACCAATCTTGCCGGCCTGGATGAGATAAATTTTAATACTGGGTTGAATACTGACCCCGTCATAGTCGAGGCCGATA
AGCTCGCCAAAGCCATTGAGAACTACCTGTCCCAGGGTGACGATATGGGAGCGGGCCTCGATGGCATGGAGGACGAGGAA
CTTGATTCCCTCAATGTTGGTGAGGACGATTCCGATCGCGGCAATGATGAAGAACCCGGTGGCGATGAAGCACCGGTAGT
TCGCTTTGTAAATAAGGTGCTTCTCGACGCAATCCGCACCGGGGCTTCAGATATCCACTTCGAACCTTATGAAAAATCCT
ACAGGGTGAGATTTCGTACCGATGGGGTCTTGCACGAAATTGCCAAGCCCCCCATCCAGCTGGCCTCCAGGCTTTCCGCA
CGCCTCAAAGTGATGTCCAAGATGGATATTTCTGAGAAGCGGGTTCCCCAAGATGGCCGCATAAAAATGAAGCTCTCCAA
AACCAAGGCTATCGATTTCCGCGTTAACAGCCTGCCGACTCTCTGGGGTGAGAAAATTGTACTGCGTATTCTCGACCCCT
CCTCTGCCAAGCTCGGCATTGATGCCCTTGGGTATGAGGATGAGCAAAAGCAAATCTATATGGACGCCTTGGCCCAACCG
CAGGGAATGATCTTAGTTACTGGCCCCACAGGATCAGGAAAGACGGTCTCGCTTTATACGGGACTTAATATCCTGAACAC
CCCGGAGCTCAATATCTCTACTGCAGAGGATCCGGTGGAGATCAATCTCGAGGGCATCAACCAGGTCAATGTTTCAGCAA
AAGTCGGACTCGACTTTGCCGAGGCCCTGCGTTCGTTCCTGCGGCAAGATCCCGATATTGTTATGGTGGGGGAAATCCGG
GACTTGGAAACGGCCGAAATCGCCATTAAGGCGGCACAAACCGGGCACTTGGTGCTTTCAACACTACACACCAACTCTGC
ACCGGAAACCCTGACTCGCCTAATGAATATGGGCGTCCCTACTTTTAATATTGCCACCTCAGTGAGCGTAATTATTGCGC
AGCGCTTGGCTCGAAGATTATGCAATGAGTGTAAGAAACCAACCACCCTTCCACCCGAAGTACTTGAGGCTGAGGGCTTT
TCCGCCGTTACAATCCCTGAAAGCGACTGGAGTATCTTTCAGCCCGTGGGTTGTGAGCACTGCTCTAAAGGCTATAAGGG
CCGCGTAGGTGTGTATGAAGTGGTTCGCATTACTGACGGCATCTCCAGAATTATAATGGAAGGAGGCAACTCTATTCAGA
TTGCAGATCAAGCTCGGCAAGAGGGCTTCAACAATCTGAGAGTTTCAGCACTGAGAAAGGTTGTGATGGGTGTCACCAGC
CTGGAGGAGGCCAACCGGGTCACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-146)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

62.172

100

0.623
  pilB Acinetobacter baylyi ADP1

59.298

100

0.593
  pilB Legionella pneumophila strain ERS1305867

53.86

100

0.539
  pilB Vibrio campbellii strain DS40M4

52.26

97.018

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

55.172

91.579

0.505
  pilB Vibrio cholerae strain A1552

52.7

94.211

0.496
  pilF Neisseria gonorrhoeae MS11

48.956

92.456

0.453
  pilB/pilB1 Synechocystis sp. PCC 6803

42.4

87.719

0.372

Multiple sequence alignment