Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACHZ97_RS03765 Genome accession   NZ_CP172310
Coordinates   827170..828894 (-) Length   574 a.a.
NCBI ID   WP_396616715.1    Uniprot ID   -
Organism   Lysobacter soli strain P18     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 822170..833894
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHZ97_RS03745 (ACHZ97_03745) - 823412..824353 (+) 942 WP_396617500.1 Nudix family hydrolase -
  ACHZ97_RS03750 (ACHZ97_03750) coaE 824366..824983 (-) 618 WP_115841428.1 dephospho-CoA kinase -
  ACHZ97_RS03755 (ACHZ97_03755) - 825030..825893 (-) 864 WP_157029412.1 A24 family peptidase -
  ACHZ97_RS03760 (ACHZ97_03760) pilC 825910..827109 (-) 1200 WP_396617501.1 type II secretion system F family protein Machinery gene
  ACHZ97_RS03765 (ACHZ97_03765) pilB 827170..828894 (-) 1725 WP_396616715.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACHZ97_RS03770 (ACHZ97_03770) - 829027..830214 (-) 1188 WP_396616716.1 glycosyltransferase family 4 protein -
  ACHZ97_RS03775 (ACHZ97_03775) - 830207..830917 (-) 711 WP_396616717.1 class I SAM-dependent methyltransferase -
  ACHZ97_RS03780 (ACHZ97_03780) - 830951..831355 (+) 405 WP_396616718.1 GtrA family protein -
  ACHZ97_RS03785 (ACHZ97_03785) - 831340..831915 (-) 576 WP_396616719.1 class I SAM-dependent methyltransferase -
  ACHZ97_RS03790 (ACHZ97_03790) - 831912..833096 (-) 1185 WP_396616720.1 glycosyltransferase family 2 protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62427.87 Da        Isoelectric Point: 6.2707

>NTDB_id=1066213 ACHZ97_RS03765 WP_396616715.1 827170..828894(-) (pilB) [Lysobacter soli strain P18]
MSTVATANLVGVTGIARRLVMDGALEEHVAREAMRAASAERKPIAVYLTEKRLVTSRAMAAANSVEFGVPLLDAAALDPA
QMAVRAVSEDLVRKHQVLPLFKRGSKLFLGIADPTNSHALDEIKFQTNLIVEPILVDQDTITRGIERALEQTDALSDAVG
DDGEGLETLDVGKDDDLSGDSGVDAKGDDTPVVKFVNKALVDAIRRGASDIHFEPYETEYRVRLRIDGILKTVAKVPVKL
QARIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRVLDGSAAKLGIDKLGYEDDQRSLFL
AAIQKPYGMVLVTGPTGSGKTVSLYTALNILNDDQRNISTVEDPVEIRVPGINQVQMNVKRGMTFAAALRSFLRQDPDVI
MVGEIRDLETAEIGIKAAQTGHMVLSTLHTNDAPQTIARLMNMGVAPYNITSSVTLVIAQRLARRLHDCKREMELPEHAL
LAEGFSHEDVAGGMKIYEPVGCPDCTEGYKGRTGIYQVMPMSEQIQAIILEGGNAMQIAEAAQKAGIRDLRQSALLKVKN
GVTSLAEINRVTKD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=1066213 ACHZ97_RS03765 WP_396616715.1 827170..828894(-) (pilB) [Lysobacter soli strain P18]
ATGTCCACGGTCGCCACGGCCAACCTGGTGGGGGTCACCGGTATTGCACGACGCTTGGTGATGGACGGCGCGCTCGAAGA
GCACGTCGCCCGCGAAGCCATGCGTGCCGCCAGTGCCGAACGTAAACCGATCGCCGTCTATCTCACCGAGAAGCGGCTGG
TCACGTCCAGGGCGATGGCCGCGGCGAACTCGGTGGAGTTCGGCGTGCCGCTGCTCGACGCCGCCGCGCTCGATCCGGCG
CAGATGGCCGTTCGCGCCGTCAGCGAGGACCTGGTGCGCAAGCACCAGGTGCTGCCGCTGTTCAAGCGTGGCAGCAAGCT
CTTCCTGGGAATCGCCGATCCCACCAATTCGCACGCGCTGGACGAGATCAAGTTCCAGACGAACCTGATCGTCGAGCCCA
TTCTGGTCGACCAGGACACGATCACGCGCGGCATCGAACGCGCGCTGGAACAGACCGACGCGCTCAGCGATGCCGTCGGC
GACGACGGTGAAGGGCTCGAAACGCTCGACGTCGGCAAGGACGACGACCTGTCCGGCGACAGCGGCGTCGACGCGAAGGG
CGACGACACGCCGGTCGTCAAGTTCGTCAACAAGGCGCTGGTGGATGCGATCCGCCGCGGCGCCTCCGACATCCACTTCG
AGCCGTACGAAACCGAGTACCGCGTGCGCTTGCGCATCGACGGCATCCTGAAGACCGTCGCCAAGGTGCCGGTGAAGCTG
CAGGCGCGCATCGCTGCGCGACTGAAGGTGATGGCGCAGTTGGACATCGCCGAGAAGCGAGTCCCGCAGGACGGTCGCAT
CAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTTCGTGTCAGCACGCTGCCGACCCTCTTCGGCGAAAAGGTGGTGC
TGCGCGTACTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTCGGTTACGAGGACGACCAGCGCAGCCTGTTCCTG
GCGGCGATCCAGAAGCCTTACGGCATGGTGCTGGTCACCGGTCCGACCGGTTCGGGCAAGACGGTGTCGCTGTACACGGC
GTTGAATATCCTCAACGACGATCAACGCAACATCTCCACGGTCGAAGACCCTGTCGAAATCCGCGTGCCCGGCATCAACC
AGGTGCAGATGAACGTCAAGCGCGGCATGACCTTCGCCGCCGCGCTGCGCAGCTTCCTGCGTCAGGACCCCGACGTGATC
ATGGTCGGCGAAATCCGCGACCTGGAAACGGCCGAGATCGGCATCAAGGCTGCGCAGACCGGCCACATGGTGCTGTCGAC
CCTGCACACCAACGACGCGCCGCAGACCATCGCCCGACTGATGAACATGGGCGTGGCGCCGTACAACATCACCTCGTCGG
TGACACTGGTGATCGCCCAGCGCCTTGCGCGTCGCCTGCACGACTGCAAGCGCGAAATGGAACTGCCCGAGCACGCGCTG
CTGGCCGAAGGCTTCTCGCACGAAGACGTCGCCGGCGGCATGAAGATCTACGAGCCCGTTGGTTGCCCCGACTGCACCGA
GGGCTACAAGGGCCGCACCGGCATCTATCAGGTCATGCCGATGTCGGAACAAATCCAGGCCATCATCCTGGAAGGCGGCA
ACGCCATGCAGATCGCCGAAGCGGCGCAGAAGGCGGGCATCCGCGACCTGCGTCAGTCCGCGCTGCTGAAAGTAAAGAAC
GGCGTCACCAGCCTCGCCGAAATCAACCGCGTCACCAAGGACTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-152)

Type II/IV secretion system protein (T2SSE)

(198-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.477

98.606

0.547
  pilB Acinetobacter baumannii D1279779

54.159

98.432

0.533
  pilB Legionella pneumophila strain ERS1305867

52.297

98.606

0.516
  pilF Neisseria gonorrhoeae MS11

46.914

98.78

0.463
  pilB Vibrio cholerae strain A1552

50.984

88.502

0.451
  pilB Vibrio parahaemolyticus RIMD 2210633

48.679

92.334

0.449
  pilB Vibrio campbellii strain DS40M4

50

89.199

0.446
  pilB/pilB1 Synechocystis sp. PCC 6803

38.519

94.077

0.362

Multiple sequence alignment