Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACH19Q_RS13930 Genome accession   NZ_CP172236
Coordinates   2935075..2935581 (-) Length   168 a.a.
NCBI ID   WP_011000517.1    Uniprot ID   Q8Y1X9
Organism   Ralstonia pseudosolanacearum strain UMN24-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2930075..2940581
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACH19Q_RS13910 (ACH19Q_13910) - 2930172..2930759 (+) 588 WP_069079304.1 DUF2946 family protein -
  ACH19Q_RS13915 (ACH19Q_13915) - 2930774..2932462 (-) 1689 WP_197368864.1 tetratricopeptide repeat protein -
  ACH19Q_RS13920 (ACH19Q_13920) moaC 2932556..2933032 (+) 477 WP_011000519.1 cyclic pyranopterin monophosphate synthase MoaC -
  ACH19Q_RS13925 (ACH19Q_13925) - 2933260..2934993 (-) 1734 WP_197368865.1 PglL family O-oligosaccharyltransferase -
  ACH19Q_RS13930 (ACH19Q_13930) pilA 2935075..2935581 (-) 507 WP_011000517.1 pilin Machinery gene
  ACH19Q_RS13935 (ACH19Q_13935) pilA 2935848..2936369 (-) 522 WP_087451617.1 pilin Machinery gene
  ACH19Q_RS13940 (ACH19Q_13940) - 2936563..2937261 (-) 699 WP_019717569.1 TerC family protein -
  ACH19Q_RS13945 (ACH19Q_13945) sucD 2937411..2938292 (-) 882 WP_011000514.1 succinate--CoA ligase subunit alpha -
  ACH19Q_RS13950 (ACH19Q_13950) sucC 2938377..2939543 (-) 1167 WP_197368866.1 ADP-forming succinate--CoA ligase subunit beta -
  ACH19Q_RS13955 (ACH19Q_13955) - 2939610..2940236 (-) 627 WP_029240215.1 DUF2889 domain-containing protein -

Sequence

Protein


Download         Length: 168 a.a.        Molecular weight: 17053.72 Da        Isoelectric Point: 9.9749

>NTDB_id=1065826 ACH19Q_RS13930 WP_011000517.1 2935075..2935581(-) (pilA) [Ralstonia pseudosolanacearum strain UMN24-1]
MKSMRHLNKRVQKGFTLIELMIVVAIVGILAAIAIPAYQDYTVRARVTEGLSLAAQAKALVSENAANAQSDLSVGSSVFT
PTKNVANLTIAGTGTVPGQITVTYTTAAGGGTLALVPTAAGTALPVSSAPSGPIMWTCYAQNKAQAASSVAPSGTMSLAA
KYAPAECR

Nucleotide


Download         Length: 507 bp        

>NTDB_id=1065826 ACH19Q_RS13930 WP_011000517.1 2935075..2935581(-) (pilA) [Ralstonia pseudosolanacearum strain UMN24-1]
ATGAAGTCGATGCGTCATCTCAACAAGCGTGTCCAGAAGGGTTTCACGCTGATCGAACTGATGATCGTGGTCGCGATCGT
CGGTATTCTGGCTGCGATTGCCATTCCGGCCTATCAGGACTACACGGTTCGTGCACGCGTGACGGAAGGTCTGTCGCTGG
CTGCGCAAGCCAAGGCGCTGGTGTCGGAAAACGCTGCCAATGCACAGTCTGACCTGTCGGTGGGTTCGTCGGTGTTCACG
CCCACCAAGAACGTTGCCAACTTGACGATTGCTGGTACGGGTACTGTTCCGGGTCAGATCACCGTCACCTATACGACGGC
AGCTGGCGGCGGCACGCTGGCTCTGGTTCCGACTGCGGCTGGTACTGCACTGCCGGTTAGCTCGGCACCGTCGGGCCCGA
TCATGTGGACCTGCTACGCTCAGAATAAGGCTCAGGCTGCTAGCAGCGTTGCTCCGAGCGGTACGATGTCGCTGGCAGCG
AAGTATGCTCCGGCAGAATGCCGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(43-168)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8Y1X9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

100

100

1
  pilA2 Legionella pneumophila strain ERS1305867

50.311

95.833

0.482
  pilA2 Legionella pneumophila str. Paris

49.689

95.833

0.476
  comP Acinetobacter baylyi ADP1

44.444

100

0.452
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36.082

100

0.417

Multiple sequence alignment