Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACHWS0_RS08435 Genome accession   NZ_CP172107
Coordinates   1774684..1775118 (-) Length   144 a.a.
NCBI ID   WP_005741096.1    Uniprot ID   -
Organism   Pasteurella multocida strain 12337023/33711123     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1769684..1780118
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHWS0_RS08405 - 1770248..1770517 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  ACHWS0_RS08410 yacG 1770517..1770720 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  ACHWS0_RS08415 coaE 1770731..1771351 (-) 621 WP_014326298.1 dephospho-CoA kinase -
  ACHWS0_RS08420 - 1771366..1772088 (-) 723 WP_014326299.1 prepilin peptidase -
  ACHWS0_RS08425 - 1772088..1773299 (-) 1212 WP_014326300.1 type II secretion system F family protein -
  ACHWS0_RS08430 pilB 1773299..1774684 (-) 1386 WP_016504529.1 GspE/PulE family protein Machinery gene
  ACHWS0_RS08435 pilA 1774684..1775118 (-) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  ACHWS0_RS08440 ampD 1775277..1775843 (+) 567 WP_014326302.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACHWS0_RS08445 - 1776429..1777052 (+) 624 WP_078836159.1 RNA pyrophosphohydrolase -
  ACHWS0_RS08450 - 1777055..1777849 (+) 795 WP_014326303.1 sulfite exporter TauE/SafE family protein -
  ACHWS0_RS08455 lgt 1777859..1778662 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  ACHWS0_RS08460 - 1778668..1779519 (+) 852 WP_005751072.1 thymidylate synthase -
  ACHWS0_RS08465 tadA 1779534..1780034 (+) 501 WP_016504277.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15058.48 Da        Isoelectric Point: 9.1281

>NTDB_id=1065241 ACHWS0_RS08435 WP_005741096.1 1774684..1775118(-) (pilA) [Pasteurella multocida strain 12337023/33711123]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1065241 ACHWS0_RS08435 WP_005741096.1 1774684..1775118(-) (pilA) [Pasteurella multocida strain 12337023/33711123]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCAGCTC
CTTATCGTGCTGAGGTAGAGCTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG
CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC
AATCTGACATTTTCCCAGCAGGATTTTGCGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.194

100

0.632
  pilA Haemophilus influenzae Rd KW20

61.111

100

0.611
  pilA Glaesserella parasuis strain SC1401

52.349

100

0.542
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.806

100

0.368
  pilA Vibrio cholerae strain A1552

36.806

100

0.368
  pilA Vibrio cholerae C6706

36.806

100

0.368

Multiple sequence alignment