Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACHWS1_RS08535 Genome accession   NZ_CP172106
Coordinates   1789405..1789839 (-) Length   144 a.a.
NCBI ID   WP_005741096.1    Uniprot ID   -
Organism   Pasteurella multocida strain 667     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1784405..1794839
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHWS1_RS08505 - 1784969..1785238 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  ACHWS1_RS08510 yacG 1785238..1785441 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  ACHWS1_RS08515 coaE 1785452..1786072 (-) 621 WP_014326298.1 dephospho-CoA kinase -
  ACHWS1_RS08520 - 1786087..1786809 (-) 723 WP_014326299.1 prepilin peptidase -
  ACHWS1_RS08525 - 1786809..1788020 (-) 1212 WP_014326300.1 type II secretion system F family protein -
  ACHWS1_RS08530 pilB 1788020..1789405 (-) 1386 WP_016504529.1 GspE/PulE family protein Machinery gene
  ACHWS1_RS08535 pilA 1789405..1789839 (-) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  ACHWS1_RS08540 ampD 1789998..1790564 (+) 567 WP_014326302.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACHWS1_RS08545 rppH 1791150..1791746 (+) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  ACHWS1_RS08550 - 1791749..1792543 (+) 795 WP_014326303.1 sulfite exporter TauE/SafE family protein -
  ACHWS1_RS08555 lgt 1792553..1793356 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  ACHWS1_RS08560 - 1793362..1794213 (+) 852 WP_005751072.1 thymidylate synthase -
  ACHWS1_RS08565 tadA 1794228..1794728 (+) 501 WP_016504277.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15058.48 Da        Isoelectric Point: 9.1281

>NTDB_id=1065219 ACHWS1_RS08535 WP_005741096.1 1789405..1789839(-) (pilA) [Pasteurella multocida strain 667]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1065219 ACHWS1_RS08535 WP_005741096.1 1789405..1789839(-) (pilA) [Pasteurella multocida strain 667]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCAGCTC
CTTATCGTGCTGAGGTAGAGCTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG
CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC
AATCTGACATTTTCCCAGCAGGATTTTGCGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.194

100

0.632
  pilA Haemophilus influenzae Rd KW20

61.111

100

0.611
  pilA Glaesserella parasuis strain SC1401

52.349

100

0.542
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.806

100

0.368
  pilA Vibrio cholerae strain A1552

36.806

100

0.368
  pilA Vibrio cholerae C6706

36.806

100

0.368

Multiple sequence alignment