Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACHWS2_RS08320 Genome accession   NZ_CP172105
Coordinates   1764428..1765813 (-) Length   461 a.a.
NCBI ID   WP_014668226.1    Uniprot ID   A0A849CCS0
Organism   Pasteurella multocida strain 680     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1759428..1770813
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHWS2_RS08290 - 1759555..1760928 (-) 1374 WP_005722571.1 alanine/glycine:cation symporter family protein -
  ACHWS2_RS08295 - 1761377..1761646 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  ACHWS2_RS08300 yacG 1761646..1761849 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  ACHWS2_RS08305 coaE 1761860..1762480 (-) 621 WP_005751078.1 dephospho-CoA kinase -
  ACHWS2_RS08310 - 1762495..1763217 (-) 723 WP_016534448.1 prepilin peptidase -
  ACHWS2_RS08315 - 1763217..1764428 (-) 1212 WP_014668225.1 type II secretion system F family protein -
  ACHWS2_RS08320 pilB 1764428..1765813 (-) 1386 WP_014668226.1 GspE/PulE family protein Machinery gene
  ACHWS2_RS08325 pilA 1765813..1766247 (-) 435 WP_014668227.1 prepilin peptidase-dependent pilin Machinery gene
  ACHWS2_RS08330 ampD 1766408..1766974 (+) 567 WP_014668228.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACHWS2_RS08335 rppH 1767560..1768027 (+) 468 Protein_1595 RNA pyrophosphohydrolase -
  ACHWS2_RS08340 - 1768159..1768953 (+) 795 WP_005719128.1 sulfite exporter TauE/SafE family protein -
  ACHWS2_RS08345 lgt 1768963..1769766 (+) 804 WP_014668230.1 prolipoprotein diacylglyceryl transferase -
  ACHWS2_RS08350 - 1769772..1770623 (+) 852 WP_014668231.1 thymidylate synthase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52108.79 Da        Isoelectric Point: 6.3686

>NTDB_id=1065195 ACHWS2_RS08320 WP_014668226.1 1764428..1765813(-) (pilB) [Pasteurella multocida strain 680]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIELVLLS
TNELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIALTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRCCDCRV
ETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=1065195 ACHWS2_RS08320 WP_014668226.1 1764428..1765813(-) (pilB) [Pasteurella multocida strain 680]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGTGCAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGTTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGTG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCTTGTTTTATTATCA
ACAAATGAACTTAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTGGAAGAAAACATACCCACGGTTTATCA
AGTTGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTTCCTTTTCAGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACATTGC
CGACACATTTAGGCGAAAAAGCGGTATTACGTTTACAGCGAAATAAGCCTATTCATCTCAGTTTTGCTGAGTTGGGGATG
ACCGCAGACCAACAACACAGATTCCAACACGCGTTAAGTCAGCCACAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGC
TCAAACAGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCCCCCTCTGCCCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTCTTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTTGTTGTGATTGTCGTGTA
GAGACACAATCGGCGTGTCGTTGCTATCAGGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATG
AAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-394)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A849CCS0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

67.991

98.265

0.668
  pilB Haemophilus influenzae Rd KW20

67.329

98.265

0.662
  pilB Glaesserella parasuis strain SC1401

58.59

98.482

0.577
  pilB Acinetobacter baumannii D1279779

39.484

100

0.432
  pilB Vibrio campbellii strain DS40M4

39.627

100

0.414
  pilB Vibrio parahaemolyticus RIMD 2210633

39.501

100

0.412
  pilB Acinetobacter baylyi ADP1

37.675

100

0.408
  pilB Vibrio cholerae strain A1552

38.477

100

0.406
  pilB Legionella pneumophila strain ERS1305867

37.219

100

0.395

Multiple sequence alignment