Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACHWS4_RS08610 Genome accession   NZ_CP172103
Coordinates   1800241..1800675 (-) Length   144 a.a.
NCBI ID   WP_005741096.1    Uniprot ID   -
Organism   Pasteurella multocida strain 682     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1795241..1805675
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHWS4_RS08580 - 1795805..1796074 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  ACHWS4_RS08585 yacG 1796074..1796277 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  ACHWS4_RS08590 coaE 1796288..1796908 (-) 621 WP_014326298.1 dephospho-CoA kinase -
  ACHWS4_RS08595 - 1796923..1797645 (-) 723 WP_014326299.1 prepilin peptidase -
  ACHWS4_RS08600 - 1797645..1798856 (-) 1212 WP_014326300.1 type II secretion system F family protein -
  ACHWS4_RS08605 pilB 1798856..1800241 (-) 1386 WP_016504529.1 GspE/PulE family protein Machinery gene
  ACHWS4_RS08610 pilA 1800241..1800675 (-) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  ACHWS4_RS08615 ampD 1800834..1801400 (+) 567 WP_014326302.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACHWS4_RS08620 rppH 1801986..1802582 (+) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  ACHWS4_RS08625 - 1802585..1803379 (+) 795 WP_014326303.1 sulfite exporter TauE/SafE family protein -
  ACHWS4_RS08630 lgt 1803389..1804192 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  ACHWS4_RS08635 - 1804198..1805049 (+) 852 WP_005751072.1 thymidylate synthase -
  ACHWS4_RS08640 tadA 1805064..1805564 (+) 501 WP_016504277.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15058.48 Da        Isoelectric Point: 9.1281

>NTDB_id=1065154 ACHWS4_RS08610 WP_005741096.1 1800241..1800675(-) (pilA) [Pasteurella multocida strain 682]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1065154 ACHWS4_RS08610 WP_005741096.1 1800241..1800675(-) (pilA) [Pasteurella multocida strain 682]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCAGCTC
CTTATCGTGCTGAGGTAGAGCTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG
CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC
AATCTGACATTTTCCCAGCAGGATTTTGCGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.194

100

0.632
  pilA Haemophilus influenzae Rd KW20

61.111

100

0.611
  pilA Glaesserella parasuis strain SC1401

52.349

100

0.542
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.806

100

0.368
  pilA Vibrio cholerae strain A1552

36.806

100

0.368
  pilA Vibrio cholerae C6706

36.806

100

0.368

Multiple sequence alignment