Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACHMXE_RS17405 Genome accession   NZ_CP172004
Coordinates   1080510..1082243 (-) Length   577 a.a.
NCBI ID   WP_203202656.1    Uniprot ID   -
Organism   Variovorax sp. UC122_21     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1075510..1087243
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHMXE_RS17385 (ACHMXE_17400) - 1076089..1077579 (-) 1491 WP_209541760.1 MBOAT family protein -
  ACHMXE_RS17390 (ACHMXE_17405) coaE 1077654..1078262 (-) 609 WP_209541759.1 dephospho-CoA kinase -
  ACHMXE_RS17395 (ACHMXE_17410) - 1078279..1079244 (-) 966 WP_395350359.1 prepilin peptidase -
  ACHMXE_RS17400 (ACHMXE_17415) pilC 1079244..1080479 (-) 1236 WP_209541757.1 type II secretion system F family protein Machinery gene
  ACHMXE_RS17405 (ACHMXE_17420) pilB 1080510..1082243 (-) 1734 WP_203202656.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACHMXE_RS17415 (ACHMXE_17430) - 1082486..1083415 (-) 930 WP_395350360.1 polyprenyl synthetase family protein -
  ACHMXE_RS17420 (ACHMXE_17435) rplU 1083663..1083974 (+) 312 WP_057592097.1 50S ribosomal protein L21 -
  ACHMXE_RS17425 (ACHMXE_17440) rpmA 1083987..1084247 (+) 261 WP_057592096.1 50S ribosomal protein L27 -
  ACHMXE_RS17430 (ACHMXE_17445) cgtA 1084319..1085395 (+) 1077 WP_395350361.1 Obg family GTPase CgtA -
  ACHMXE_RS17435 (ACHMXE_17450) proB 1085474..1086612 (+) 1139 Protein_1036 glutamate 5-kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62648.90 Da        Isoelectric Point: 6.0490

>NTDB_id=1064773 ACHMXE_RS17405 WP_203202656.1 1080510..1082243(-) (pilB) [Variovorax sp. UC122_21]
MAAAELPVKENPQVALPGLARALVSAGKLPVKTAEDLYQKSLSSRSSFIAELTGTGAVSASDLAHTLSNAFGAPLLDLDA
IDHQRLPKDLLDPKLCQAYRIVVLSKRNNRLIVATADPSDQQAAEKIKFASQMGVDWVIAEYDKLSRMVEAAAASAAETI
NSIVGTEFEFDDVSADTAGDANEQAIAEVEDAPVVRFLHKMLLDAVSMRASDIHFEPYEHHYRVRFRVDGELREVASPPT
VIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVVRILDPSSARMGIDALGYDADEKER
LLHAISRPYGMVLVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAAALRAFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCMTCRTPADIPR
EALIEAGFKEHEVDGSWKPYRPVGCAACTGGYKGRVGIYQVMPISEAIQEIILRDGSALDIAQQSEAEGVRSLRKSGLRK
VMQGLTSLEEVVAVTNL

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1064773 ACHMXE_RS17405 WP_203202656.1 1080510..1082243(-) (pilB) [Variovorax sp. UC122_21]
ATGGCTGCAGCCGAACTTCCCGTTAAAGAAAACCCGCAAGTCGCCTTGCCAGGGCTGGCACGCGCGCTGGTTTCGGCGGG
CAAGCTGCCGGTGAAGACGGCGGAGGATCTTTATCAGAAGTCGCTGAGCAGCCGCTCGAGCTTCATCGCCGAGCTCACGG
GCACCGGCGCCGTCTCGGCCTCCGACCTCGCCCACACGCTGTCGAATGCGTTCGGCGCCCCGCTGCTCGACCTCGACGCC
ATCGACCACCAGCGCCTGCCGAAGGACCTGCTCGATCCCAAGCTCTGCCAGGCCTACCGCATCGTCGTCCTCAGCAAGCG
CAACAACCGCCTGATCGTCGCCACGGCCGATCCGTCCGACCAGCAGGCCGCCGAGAAGATCAAGTTCGCCTCCCAGATGG
GCGTGGACTGGGTCATCGCCGAGTACGACAAGCTCTCGCGCATGGTCGAGGCCGCCGCCGCGAGCGCGGCCGAAACCATC
AACAGCATCGTCGGCACGGAGTTCGAGTTCGACGACGTCAGCGCCGACACCGCGGGCGACGCCAATGAGCAGGCCATCGC
CGAGGTCGAGGACGCGCCTGTCGTGCGCTTCCTGCACAAGATGCTGCTCGACGCGGTCAGCATGCGCGCGTCGGACATCC
ACTTCGAGCCCTACGAGCACCACTACCGCGTGCGCTTCCGGGTCGACGGCGAACTGCGCGAGGTGGCGAGCCCGCCCACG
GTGATCAAGGACAAGCTGGCGTCGCGCATCAAGGTCATCTCGCGGCTCGACATCTCCGAGAAGCGCGTGCCCCAGGACGG
GCGCATGAAGCTCAAGATCGGCCCCGACCGGGTGATCGACTTCCGCGTGAGCACGCTGCCCACGCTGTTCGGCGAGAAGA
TCGTGGTCCGGATTCTCGACCCCAGCAGCGCGCGCATGGGCATCGATGCGCTGGGCTACGACGCCGACGAGAAGGAACGC
CTGCTGCACGCCATCAGCCGCCCCTACGGCATGGTGCTCGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGCTCTA
CACCTGCCTCAACCTGCTGAACCAGCCGGGCGTGAACATCGCGACCGCCGAGGACCCGTCGGAAATCAACCTGCCGGGCG
TCAACCAGGTCAATGTGAACGAGAAGGCCGGCCTGACCTTCGCGGCCGCGCTGCGGGCCTTCCTGCGGCAGGATCCCGAC
ATCATCATGGTCGGCGAAATCCGCGACCTCGAAACCGCCGACATCTCGATCAAGGCCGCCCAGACCGGCCACCTGGTGCT
GTCGACGCTGCACACCAACGACGCCCCCACCACCCTCACGCGGATGCGCAACATGGGCATCGCGCCCTTCAACATCGCAT
CGAGCGTGATCCTGATCACCGCCCAACGGCTCGCGCGGCGGCTGTGCATGACCTGCCGCACGCCCGCGGACATCCCGCGC
GAGGCGCTGATCGAGGCCGGCTTCAAGGAGCACGAGGTCGACGGCAGCTGGAAGCCCTACCGGCCCGTGGGCTGCGCGGC
CTGCACCGGCGGCTACAAGGGCCGGGTCGGCATCTACCAGGTGATGCCGATCTCCGAGGCGATCCAGGAGATCATCCTGC
GCGACGGCAGCGCGCTCGACATCGCGCAGCAGTCGGAAGCCGAAGGCGTGCGCTCGCTGCGCAAGTCGGGCCTGCGCAAA
GTGATGCAGGGCCTCACCTCGCTCGAGGAAGTGGTGGCGGTCACCAACCTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-156)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.545

97.227

0.53
  pilB Acinetobacter baylyi ADP1

53.086

98.267

0.522
  pilF Neisseria gonorrhoeae MS11

52.847

97.4

0.515
  pilB Legionella pneumophila strain ERS1305867

49.113

97.747

0.48
  pilB Vibrio campbellii strain DS40M4

45.745

97.747

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

45.907

97.4

0.447
  pilB Vibrio cholerae strain A1552

47.5

90.121

0.428
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.643

91.681

0.373
  pilF Thermus thermophilus HB27

37.566

98.267

0.369

Multiple sequence alignment