Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACHMXC_RS03460 Genome accession   NZ_CP171997
Coordinates   767926..769665 (+) Length   579 a.a.
NCBI ID   WP_056060313.1    Uniprot ID   -
Organism   Acidovorax delafieldii strain UC81_30     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 762926..774665
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHMXC_RS03430 (ACHMXC_03430) proB 763452..764594 (-) 1143 WP_395456230.1 glutamate 5-kinase -
  ACHMXC_RS03435 (ACHMXC_03435) cgtA 764685..765754 (-) 1070 Protein_674 Obg family GTPase CgtA -
  ACHMXC_RS03440 (ACHMXC_03440) rpmA 765925..766182 (-) 258 WP_005794180.1 50S ribosomal protein L27 -
  ACHMXC_RS03445 (ACHMXC_03445) rplU 766198..766509 (-) 312 WP_005794184.1 50S ribosomal protein L21 -
  ACHMXC_RS03450 (ACHMXC_03450) - 766745..767674 (+) 930 WP_395456231.1 polyprenyl synthetase family protein -
  ACHMXC_RS03460 (ACHMXC_03460) pilB 767926..769665 (+) 1740 WP_056060313.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACHMXC_RS03465 (ACHMXC_03465) pilC 769686..770906 (+) 1221 WP_146869859.1 type II secretion system F family protein Machinery gene
  ACHMXC_RS03470 (ACHMXC_03470) - 770906..771778 (+) 873 WP_395456234.1 prepilin peptidase -
  ACHMXC_RS03475 (ACHMXC_03475) coaE 771788..772408 (+) 621 WP_395456236.1 dephospho-CoA kinase -
  ACHMXC_RS03480 (ACHMXC_03480) zapD 772494..773249 (+) 756 WP_056060302.1 cell division protein ZapD -
  ACHMXC_RS03485 (ACHMXC_03485) - 773339..773557 (+) 219 WP_082522383.1 DNA gyrase inhibitor YacG -
  ACHMXC_RS03490 (ACHMXC_03490) - 773675..774133 (-) 459 WP_099732210.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 63052.42 Da        Isoelectric Point: 5.2425

>NTDB_id=1064747 ACHMXC_RS03460 WP_056060313.1 767926..769665(+) (pilB) [Acidovorax delafieldii strain UC81_30]
MAAVDTALKEASAVALPGLGRALMSAGKLTQKSAEDIYKKSQISRTSFIAELTGSGAVSAADLAHTVSAVFGAPLLDLDA
IDPLRLPKELLDNKICQAYRVVVLSKRNNRLIVATADPTDQEAAEKIKFTTQMGVDWIIAEYDKLSRLVEATTKSASESM
DTLISGGGDFAFDDVSIEDAPEEADTGTTEVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIASP
PIAIKDKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPVEK
ERLLQAIGRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGMTFAVALKSFLRQD
PDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPVCKVPADI
PHETLVEAGYEEHDIDGSWVTYRPVGCSACNNGYKGRLGIYQVMPISEEIQRIILRDGSALEIAEQARAEGVRSLRESGL
YKAKLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=1064747 ACHMXC_RS03460 WP_056060313.1 767926..769665(+) (pilB) [Acidovorax delafieldii strain UC81_30]
ATGGCCGCTGTTGATACTGCCCTCAAAGAAGCCTCTGCAGTAGCCCTGCCCGGGCTCGGCCGCGCCCTGATGTCTGCGGG
CAAGCTGACCCAAAAGTCAGCAGAAGACATTTACAAAAAATCACAAATAAGCCGAACCAGTTTCATTGCCGAGCTGACGG
GCTCTGGAGCTGTTTCTGCAGCGGATCTGGCGCACACGGTATCTGCGGTTTTCGGCGCACCCCTGCTCGACTTGGACGCC
ATTGATCCTTTGCGCCTGCCGAAGGAGCTCTTGGATAACAAGATATGCCAAGCATATCGCGTGGTGGTCCTGAGCAAGCG
CAACAACCGGCTGATCGTCGCAACAGCCGACCCGACTGACCAAGAAGCTGCAGAGAAAATCAAATTCACCACCCAAATGG
GTGTGGATTGGATTATTGCGGAATATGACAAACTCAGCCGGCTCGTAGAAGCCACCACCAAATCCGCAAGCGAATCCATG
GATACGCTGATCAGCGGAGGAGGTGACTTCGCTTTCGATGACGTCTCCATTGAAGATGCGCCTGAAGAAGCGGATACAGG
AACAACGGAAGTTGAAGATGCGCCGATCGTCAAGTTCCTGCACAAAATGCTGCTCGACGCCTTCAATATGCGCGCCTCCG
ATTTGCATTTTGAGCCTTACGAACACCAATACCGAGTCCGTTTCCGCATTGACGGAGAACTCCGCGAAATAGCCTCTCCA
CCGATCGCGATCAAGGACAAGCTCGCGTCCCGCATCAAGGTGATTTCACGCCTAGATATTTCCGAGAAGCGTGTGCCTCA
GGACGGTCGCATGAAGCTCAAGGTGGGGCCGGATCGCGTGATCGATTTTCGGGTAAGCACTTTGCCCACCCTGTTTGGCG
AGAAAATCGTGATCCGTATTTTGGATCCGAGTAGCGCCAAGCTTGGGATCGATGCACTTGGCTATGAACCCGTGGAGAAG
GAACGATTGCTCCAGGCGATTGGTCGTCCTTACGGAATGATTCTGGTCACAGGCCCTACGGGCTCTGGCAAGACGGTATC
TTTATACACCTGTCTGAATCTGCTGAACAAGCCGGGGGTCAATATCGCAACCGCTGAAGACCCTTCTGAAATCAATTTGC
CTGGTGTCAATCAGGTGAACGTGAATGAAAAAGCCGGCATGACCTTTGCTGTGGCACTGAAGTCGTTTCTGCGCCAGGAT
CCCGACATCATCATGGTGGGTGAAATCCGGGATCTGGAAACTGCAGATATTTCCATCAAGGCGGCGCAGACAGGTCACCT
TGTGCTTTCCACGCTGCACACGAACGACGCGCCCACGACGCTCACGCGGATGCGCAACATGGGTATAGCGCCGTTCAACA
TCGCTTCCAGCGTGATTTTGATCACTGCGCAACGTTTGGCGCGTCGATTGTGCCCCGTATGCAAGGTTCCCGCAGATATA
CCCCATGAGACTTTGGTGGAGGCGGGATACGAGGAACATGACATTGACGGCTCCTGGGTGACCTATCGTCCCGTAGGATG
CTCGGCCTGCAACAACGGCTACAAGGGCCGACTCGGCATCTATCAAGTAATGCCTATTTCCGAGGAGATCCAGCGCATCA
TTTTGCGCGACGGGAGTGCACTGGAGATTGCAGAGCAGGCACGCGCCGAGGGTGTTCGTTCGCTGCGCGAATCCGGGCTC
TACAAAGCCAAATTGGGCCTGACTTCCCTTGAGGAAGTTCTGGCCGTCACCAACGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-152)

Type II/IV secretion system protein (T2SSE)

(202-469)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.59

98.618

0.528
  pilB Acinetobacter baumannii D1279779

52.048

100

0.527
  pilF Neisseria gonorrhoeae MS11

52.212

97.582

0.509
  pilB Legionella pneumophila strain ERS1305867

49.823

97.409

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

45.15

97.927

0.442
  pilB Vibrio campbellii strain DS40M4

44.991

98.273

0.442
  pilB Vibrio cholerae strain A1552

46.555

92.746

0.432
  pilF Thermus thermophilus HB27

39.456

100

0.401
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.161

98.791

0.387

Multiple sequence alignment