Detailed information NTG1063387

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACGLYC_RS12430
• Genome accession: NZ_CP171691
• Coordinates: 2550157..2550771 (-)
• Length: 204 a.a.
• NCBI ID: WP_032866693.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain K12
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2545157..2555771

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACGLYC_RS12400 (ACGLYC_12400)	rpsT	2545460..2545726 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACGLYC_RS12405 (ACGLYC_12405)	holA	2545743..2546784 (-)	1042	Protein_2396	DNA polymerase III subunit delta	-
ACGLYC_RS12410 (ACGLYC_12410)	-	2546819..2546975 (-)	157	Protein_2397	hypothetical protein	-
ACGLYC_RS12415 (ACGLYC_12415)	-	2547016..2547150 (+)	135	WP_003152870.1	YqzM family protein	-
ACGLYC_RS12420 (ACGLYC_12420)	comEC	2547169..2549520 (-)	2352	WP_032866696.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACGLYC_RS12425 (ACGLYC_12425)	-	2549521..2550090 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACGLYC_RS12430 (ACGLYC_12430)	comEA	2550157..2550771 (-)	615	WP_032866693.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACGLYC_RS12435 (ACGLYC_12435)	comER	2550830..2551651 (+)	822	WP_032866690.1	late competence protein ComER	-
ACGLYC_RS12440 (ACGLYC_12440)	-	2551720..2552457 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
ACGLYC_RS12445 (ACGLYC_12445)	rsfS	2552454..2552810 (-)	357	WP_007408260.1	ribosome silencing factor	-
ACGLYC_RS12450 (ACGLYC_12450)	yqeK	2552828..2553388 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACGLYC_RS12455 (ACGLYC_12455)	-	2553378..2553947 (-)	570	WP_032866687.1	nicotinate-nucleotide adenylyltransferase	-
ACGLYC_RS12460 (ACGLYC_12460)	yhbY	2553958..2554248 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ACGLYC_RS12465 (ACGLYC_12465)	aroE	2554242..2555084 (-)	843	WP_007408256.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22151.28 Da
• Isoelectric Point: 6.4858

>NTDB_id=1063387 ACGLYC_RS12430 WP_032866693.1 2550157..2550771(-) (comEA) [Bacillus velezensis strain K12]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSDKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1063387 ACGLYC_RS12430 WP_032866693.1 2550157..2550771(-) (comEA) [Bacillus velezensis strain K12]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGACAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTTAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCATACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363