Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACFN7Z_RS15850 Genome accession   NZ_CP171157
Coordinates   3219725..3221437 (+) Length   570 a.a.
NCBI ID   WP_010325768.1    Uniprot ID   A0AAW6RVG1
Organism   Acinetobacter sp. P1332     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3214725..3226437
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFN7Z_RS15805 (ACFN7Z_15805) nusA 3215308..3216792 (-) 1485 WP_004692706.1 transcription termination factor NusA -
  ACFN7Z_RS15810 (ACFN7Z_15810) rimP 3216832..3217356 (-) 525 WP_004692708.1 ribosome maturation factor RimP -
  ACFN7Z_RS15840 (ACFN7Z_15840) secG 3218311..3218640 (-) 330 WP_004692710.1 preprotein translocase subunit SecG -
  ACFN7Z_RS15845 (ACFN7Z_15845) tpiA 3218654..3219445 (-) 792 WP_010325769.1 triose-phosphate isomerase -
  ACFN7Z_RS15850 (ACFN7Z_15850) pilB 3219725..3221437 (+) 1713 WP_010325768.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACFN7Z_RS15855 (ACFN7Z_15855) pilC 3221468..3222694 (+) 1227 WP_010325767.1 type II secretion system F family protein Machinery gene
  ACFN7Z_RS15860 (ACFN7Z_15860) pilD 3222694..3223554 (+) 861 WP_010325766.1 A24 family peptidase Machinery gene
  ACFN7Z_RS15865 (ACFN7Z_15865) coaE 3223561..3224160 (+) 600 WP_010325765.1 dephospho-CoA kinase -
  ACFN7Z_RS15870 (ACFN7Z_15870) - 3224175..3225092 (-) 918 WP_029574926.1 DMT family transporter -
  ACFN7Z_RS15875 (ACFN7Z_15875) rlmB 3225125..3225874 (-) 750 WP_004692717.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACFN7Z_RS15880 (ACFN7Z_15880) - 3226017..3226340 (-) 324 WP_004692718.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63577.86 Da        Isoelectric Point: 5.0082

>NTDB_id=1060383 ACFN7Z_RS15850 WP_010325768.1 3219725..3221437(+) (pilB) [Acinetobacter sp. P1332]
MSVSNGLPKFTGFIRRLVEDGRVTAENMQQAMLSAKKAQQDIVPYLIENHKLSPQMIAETISLEFGEPVFDLSVYDSVQI
LRDLVEDKLITKHRVLPIYRRGNILYIATSNPTNMDAMDAIRFNSKMNIEAIIVEHPQLEKLIEQNFAQADSFEFSDEDI
DLDLEQDHQNSNDEEDDSPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRHIASPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKDLFMQALDKP
QGMLLITGPTGSGKTVSLYTGLNILNKEDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKASVDIPEQSLLEMGF
TSTDIKDPDFKVYQPVGCAECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAEASAKLGFNNLRRSGLLKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1060383 ACFN7Z_RS15850 WP_010325768.1 3219725..3221437(+) (pilB) [Acinetobacter sp. P1332]
ATGTCAGTATCAAATGGTTTGCCTAAGTTCACAGGCTTCATTCGACGCTTAGTCGAAGACGGACGCGTGACTGCTGAAAA
TATGCAGCAGGCAATGCTCTCTGCAAAAAAGGCTCAGCAAGACATCGTTCCTTATCTGATTGAAAACCATAAACTCTCCC
CACAAATGATCGCTGAGACGATTTCTTTGGAGTTTGGAGAGCCTGTTTTTGACTTAAGTGTCTATGATTCTGTACAAATT
CTACGGGATCTGGTCGAAGACAAACTTATCACCAAGCATCGTGTGTTGCCGATTTACCGTCGTGGCAATATTCTCTATAT
CGCTACCAGCAATCCAACCAACATGGATGCGATGGACGCGATTCGCTTCAATTCTAAAATGAACATTGAAGCGATTATTG
TTGAACATCCCCAGCTTGAAAAACTGATCGAACAAAATTTCGCGCAAGCTGATAGCTTTGAATTTTCTGATGAAGATATT
GATTTAGACTTAGAGCAAGACCATCAGAACTCAAATGACGAAGAAGATGACTCACCTCAAGGGGATGAAGCGCCGATCGT
CAAATACATCAATAAATTACTGATCGATGCGATTCGTATGGGTGCATCAGACTTGCACTTTGAACCTTATGAAAAATCTT
ACCGTGTCCGTTATCGTGTAGATGGAGTTCTGCGTCATATTGCCAGTCCACCACTACAACTGGCGACTCGCCTCGCCTCT
CGTTTAAAAGTCATGTCACAAATGGACATTTCTGAAAAGCGCGTGCCACAAGATGGCCGAATTAAATTAAAGCTGTCAAA
AAGTAAAGCCATCGACTTTCGTGTCAACTCACTTCCAACCTTGTTTGGCGAAAAACTGGTTCTGCGTATTCTCGATCCAT
CCAGTGCTATGCTGGGGATTGATGCTTTAGGTTATGAACCAGACCAAAAAGATCTGTTTATGCAGGCTTTAGACAAACCT
CAAGGCATGTTGCTAATTACTGGCCCGACAGGTTCAGGTAAAACGGTATCTCTATATACAGGTCTGAATATTCTAAATAA
AGAAGACACCAATATCTCAACTGCGGAAGATCCTGTTGAGATTAACTTAGAAGGCATTAATCAGGTTAACGTGAACAATA
AAGTCGGCCTTACCTTCTCTGCTGCGCTGAAGTCCTTCTTACGTCAGGATCCTGATATTGTGATGGTCGGTGAGATTCGT
GACCTTGAAACAGCTGAAATCGCGATTAAAGCCGCGCAGACAGGCCACATGGTCATGTCGACACTCCACACCAATAGTGC
ACCTGAAACTCTGACACGCTTACGCAATATGGGGGTTCCCTCTTTTAACATTGCGACCTCGGTCAATTTGGTAATTGCAC
AACGTTTAGCACGCCGCTTATGTAGCCAATGCAAAGCCTCTGTCGATATTCCTGAACAAAGCTTGCTCGAAATGGGCTTT
ACTTCCACAGATATCAAAGATCCAGACTTCAAAGTTTATCAACCGGTGGGGTGTGCTGAGTGCCGTGAAGGCTATAAAGG
ACGTGTTGGTATTTATGAAGTCATGAAAGTCACACCAGAAATTTCAAAAATTATTATGGAAGATGGCAATGCCTTACAGA
TTGCGGAAGCATCTGCAAAATTAGGATTTAACAATTTACGTCGTTCAGGTTTACTCAAGGTTATGCAGGGGGTCACTTCC
TTACAAGAAGTGAATCGTGTAACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.404

100

0.814
  pilB Acinetobacter baylyi ADP1

76.842

100

0.768
  pilB Legionella pneumophila strain ERS1305867

53.933

93.684

0.505
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio cholerae strain A1552

48.325

99.474

0.481
  pilB Vibrio campbellii strain DS40M4

47.849

97.895

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

50.677

90.702

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

34.41

100

0.374
  pilF Thermus thermophilus HB27

36.602

100

0.367

Multiple sequence alignment