Detailed information NTG1060372

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACFN7Z_RS08960
• Genome accession: NZ_CP171157
• Coordinates: 1842074..1843267 (-)
• Length: 397 a.a.
• NCBI ID: WP_010326644.1
• Uniprot ID: A0A239RZK9
• Organism: Acinetobacter sp. P1332
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1837074..1848267

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFN7Z_RS08945 (ACFN7Z_08945)	-	1839115..1840143 (+)	1029	WP_004693238.1	AraC family transcriptional regulator	-
ACFN7Z_RS08950 (ACFN7Z_08950)	gcvH	1840215..1840595 (+)	381	WP_058951997.1	glycine cleavage system protein GcvH	-
ACFN7Z_RS08955 (ACFN7Z_08955)	-	1840848..1842026 (+)	1179	Protein_1732	MFS transporter	-
ACFN7Z_RS08960 (ACFN7Z_08960)	pilU	1842074..1843267 (-)	1194	WP_010326644.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACFN7Z_RS08965 (ACFN7Z_08965)	-	1843507..1844568 (-)	1062	WP_010326643.1	3-deoxy-7-phosphoheptulonate synthase	-
ACFN7Z_RS08970 (ACFN7Z_08970)	-	1844547..1844660 (+)	114	WP_157843581.1	hypothetical protein	-
ACFN7Z_RS08975 (ACFN7Z_08975)	-	1845047..1847146 (-)	2100	WP_130235421.1	EAL domain-containing protein	-
ACFN7Z_RS08980 (ACFN7Z_08980)	-	1847405..1847728 (+)	324	WP_032031561.1	IS5 family transposase	-
ACFN7Z_RS08985 (ACFN7Z_08985)	-	1847766..1848158 (+)	393	Protein_1738	transposase family protein	-

Sequence

Protein

• Length: 397 a.a.
• Molecular weight: 44702.08 Da
• Isoelectric Point: 6.5063

>NTDB_id=1060372 ACFN7Z_RS08960 WP_010326644.1 1842074..1843267(-) (pilU) [Acinetobacter sp. P1332]
MYSAELLEEARRMMFHMLSKVVEYGGSDLFISADFPPSIKHQGLMKPLGQQNLPSDQTKLFAYSLMNEKQRLEFETELEC
NFAINVPNVSRFRVNVFQQQLHVGMVIRTITAEIPNFTKLQLPTSLKDVIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIINFFPDERRNQLLMDLSSNMKAIISQRLVRTEDGRGRRAAVEIMLNTPLMSDLILKGNFHELKEVMSKS
RELGMQTFDQALFDLYNQGAIAYEEALRNADSVNELRLQIKLKSSRANPQLNSNSALSFDQAVAEKAKDAEEEKADA

Nucleotide

• Length: 1194 bp

>NTDB_id=1060372 ACFN7Z_RS08960 WP_010326644.1 1842074..1843267(-) (pilU) [Acinetobacter sp. P1332]
ATGTATTCTGCAGAACTTTTAGAAGAAGCCCGTCGGATGATGTTTCACATGCTCAGCAAAGTGGTTGAGTATGGTGGTTC
AGACTTATTTATATCTGCAGACTTTCCGCCAAGTATTAAGCATCAGGGCCTCATGAAGCCATTGGGTCAACAAAATTTGC
CATCTGATCAGACCAAACTCTTTGCTTATAGTTTAATGAACGAAAAGCAACGATTGGAGTTCGAAACCGAGCTTGAGTGT
AATTTTGCAATTAACGTTCCCAATGTTTCGCGTTTTCGTGTCAATGTTTTTCAGCAGCAACTGCATGTGGGGATGGTGAT
TCGTACGATTACTGCGGAAATTCCTAACTTCACTAAGTTACAACTTCCAACGTCTTTAAAAGACGTAATTATGGAAAAAC
GTGGTTTGGTCCTCGTTGTCGGTGGTACAGGGTCGGGTAAATCGACGTCTTTAGCAGCCATGATTGATCATCGAAATGAA
AACTCGGCAGGGCACATTATTACAGTTGAAGACCCTGTGGAGTATGTACACAAGCATAAGAAATCGATGATTACTCATCG
GGAAGTGGGTGTGGACTGTCATTCTTGGCACAATGCTTTGAAAAATACTCTGCGCCAAGCGCCCGATGTCATTTTAATTG
GTGAGATTCGTGACACGGAGACGATGGAACATGCCATAGCTTTTGCGGAAACAGGCCACTTATGCCTGGGGACACTGCAT
GCCAATAATGCAAATCAGGCTTTAGATCGAATTATTAACTTTTTCCCAGACGAACGTCGCAATCAACTTTTGATGGACTT
ATCTTCCAATATGAAGGCGATTATTTCACAGCGTCTGGTGCGAACTGAAGATGGGCGTGGCCGCCGAGCAGCAGTGGAAA
TCATGCTCAATACACCGTTGATGTCAGACTTGATACTTAAAGGGAATTTCCACGAACTCAAAGAAGTGATGTCGAAATCA
CGAGAGTTAGGCATGCAGACTTTTGATCAGGCTTTGTTTGATTTATACAATCAAGGTGCAATCGCTTATGAAGAAGCACT
ACGAAATGCAGACTCGGTCAATGAATTACGTCTACAGATTAAATTAAAGAGTAGTCGTGCCAACCCGCAGTTAAACTCAA
ATAGTGCTTTGTCTTTTGATCAAGCGGTTGCTGAAAAAGCCAAAGACGCTGAAGAAGAGAAGGCCGACGCCTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (122-282)

3D structure

• AlphaFold DB: A0A239RZK9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	59.053	90.428	0.534
pilU	Acinetobacter baylyi ADP1	55.096	91.436	0.504
pilU	Vibrio cholerae strain A1552	50.578	87.154	0.441
pilT	Legionella pneumophila strain Lp02	42.478	85.39	0.363
pilT	Legionella pneumophila strain ERS1305867	42.478	85.39	0.363
pilT	Acinetobacter baylyi ADP1	43.598	82.62	0.36