Detailed information NTG1060128

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACF3NW_RS10855
• Genome accession: NZ_CP171110
• Coordinates: 2112512..2113681 (+)
• Length: 389 a.a.
• NCBI ID: WP_314253157.1
• Uniprot ID: -
• Organism: Eikenella halliae strain WGS1533
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2107512..2118681

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACF3NW_RS10835 (ACF3NW_10835)	-	2108660..2109355 (-)	696	WP_314253163.1	OmpA family protein	-
ACF3NW_RS10840 (ACF3NW_10840)	-	2109799..2110743 (+)	945	WP_314253161.1	LysR substrate-binding domain-containing protein	-
ACF3NW_RS10845 (ACF3NW_10845)	mtgA	2110926..2111696 (+)	771	WP_314253159.1	monofunctional biosynthetic peptidoglycan transglycosylase	-
ACF3NW_RS10850 (ACF3NW_10850)	-	2111965..2112408 (+)	444	WP_392423330.1	universal stress protein	-
ACF3NW_RS10855 (ACF3NW_10855)	pilU	2112512..2113681 (+)	1170	WP_314253157.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACF3NW_RS10860 (ACF3NW_10860)	fabG	2113978..2114706 (+)	729	WP_392423331.1	3-oxoacyl-ACP reductase FabG	-
ACF3NW_RS10865 (ACF3NW_10865)	-	2114706..2115929 (+)	1224	WP_314253415.1	beta-ketoacyl-ACP synthase	-
ACF3NW_RS10870 (ACF3NW_10870)	-	2116227..2117390 (+)	1164	WP_314253417.1	efflux RND transporter periplasmic adaptor subunit	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42876.14 Da
• Isoelectric Point: 5.4411

>NTDB_id=1060128 ACF3NW_RS10855 WP_314253157.1 2112512..2113681(+) (pilU) [Eikenella halliae strain WGS1533]
MAIPAYNEEMKKFIHGLLNHLVQSKGSDLFITAGFPPAMKLDGKLTPITDKPLTADHTALIARALMDDKQAEEFDNTKEC
NFAISLAGVSRFRINAMVQRGAAALVCRVITSQIPKFDNMNLPPILKQVVMEKRGLVIFVGGTGSGKSTSLAAMIDYRNE
NSHGHIITIEDPIEFVHPHKNCIITQREVGVDTENWFAALKNTLRQAPDVILIGEIRDRETMDYALAFAETGHLCMATLH
ANNSNQALDRIINFFPEERRTQLLNDLSLNLKGFISQRLVPKPDGKGRVAAVEVLLNSPLIAELILNGDIHGVKEIMARS
RDLGMQTFDQSLFDLYEEGLIAYDDALKNADSVNDLRLNIQLNSKRGNAGQASGIDSLAIVGMDEAEEA

Nucleotide

• Length: 1170 bp

>NTDB_id=1060128 ACF3NW_RS10855 WP_314253157.1 2112512..2113681(+) (pilU) [Eikenella halliae strain WGS1533]
ATGGCAATCCCCGCTTACAACGAAGAGATGAAAAAATTCATCCACGGCCTGCTCAACCACCTGGTGCAGAGCAAAGGTTC
CGACCTGTTCATCACCGCCGGCTTCCCCCCGGCCATGAAGCTCGACGGCAAGCTCACCCCGATTACCGACAAACCGCTCA
CCGCCGACCACACCGCCCTCATCGCCCGCGCCCTGATGGACGATAAACAGGCCGAAGAATTCGACAACACCAAAGAATGC
AACTTCGCCATCAGCCTGGCCGGCGTATCCCGCTTCCGCATCAACGCCATGGTGCAGCGCGGCGCCGCCGCCCTCGTGTG
CCGCGTGATCACCAGCCAGATTCCCAAGTTCGACAACATGAACCTGCCGCCGATTTTGAAACAAGTGGTGATGGAAAAAC
GCGGCCTCGTGATTTTTGTGGGCGGTACCGGCTCCGGCAAATCCACCTCCCTGGCCGCCATGATCGACTACCGCAACGAA
AACAGCCACGGCCACATCATCACCATCGAAGACCCGATCGAATTCGTGCACCCGCACAAAAACTGCATCATCACCCAGCG
CGAAGTGGGCGTGGACACCGAAAACTGGTTTGCCGCCCTCAAAAACACCCTGCGCCAAGCCCCCGACGTCATCCTCATCG
GCGAAATCCGCGACCGCGAAACCATGGACTACGCCCTGGCCTTCGCCGAAACCGGCCACCTCTGCATGGCCACCCTGCAC
GCCAACAACTCTAACCAGGCGCTCGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGCACCCAGCTGCTGAACGACCT
CTCGCTCAACCTCAAAGGCTTCATTTCCCAGCGCCTCGTGCCCAAGCCCGACGGCAAAGGCCGCGTGGCCGCCGTGGAAG
TGCTGCTCAATTCCCCGCTGATTGCCGAGCTGATTTTGAACGGCGACATCCACGGCGTGAAAGAAATCATGGCCCGCTCC
CGCGATTTGGGCATGCAAACCTTCGACCAATCCCTGTTCGACCTCTACGAAGAAGGCCTGATTGCCTACGATGACGCCCT
GAAAAACGCCGATTCGGTAAACGACCTGCGCCTCAACATCCAGCTCAACAGCAAGCGCGGCAATGCCGGCCAGGCATCCG
GTATCGACAGCTTGGCGATTGTGGGTATGGACGAGGCAGAAGAAGCCTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (103-259)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.318	89.717	0.55
pilU	Acinetobacter baylyi ADP1	59.143	89.974	0.532
pilU	Vibrio cholerae strain A1552	55.278	92.545	0.512
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.675	85.347	0.373
pilT	Legionella pneumophila strain Lp02	42.643	85.604	0.365
pilT	Legionella pneumophila strain ERS1305867	42.643	85.604	0.365