Detailed information NTG0105998

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: AAA985_RS10425
• Genome accession: NZ_AP028603
• Coordinates: 1978767..1979228 (-)
• Length: 153 a.a.
• NCBI ID: WP_000250540.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae strain Pne2
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1973767..1984228

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAA985_RS10390 (TKY181970_20260)	-	1974071..1974895 (-)	825	WP_001832473.1	membrane protein insertase YidC	-
AAA985_RS10395 (TKY181970_20270)	rnpA	1974870..1975241 (-)	372	WP_000739244.1	ribonuclease P protein component	-
AAA985_RS10400	-	1975258..1975389 (-)	132	WP_000768904.1	hypothetical protein	-
AAA985_RS10405 (TKY181970_20280)	-	1975390..1976580 (-)	1191	WP_000167752.1	acetate kinase	-
AAA985_RS10410 (TKY181970_20290)	comYH	1976631..1977584 (-)	954	WP_000345126.1	class I SAM-dependent methyltransferase	Machinery gene
AAA985_RS10415	-	1977645..1978239 (-)	595	Protein_2027	methyltransferase domain-containing protein	-
AAA985_RS10420 (TKY181970_20320)	comGG/cglG	1978376..1978789 (-)	414	WP_000265620.1	competence type IV pilus minor pilin ComGG	Machinery gene
AAA985_RS10425 (TKY181970_20330)	comGF/cglF	1978767..1979228 (-)	462	WP_000250540.1	competence type IV pilus minor pilin ComGF	Machinery gene
AAA985_RS10430 (TKY181970_20340)	comGE/cglE	1979191..1979493 (-)	303	WP_000413382.1	competence type IV pilus minor pilin ComGE	Machinery gene
AAA985_RS10435 (TKY181970_20350)	comGD/cglD	1979456..1979860 (-)	405	WP_000588023.1	competence type IV pilus minor pilin ComGD	Machinery gene
AAA985_RS10440 (TKY181970_20360)	comGC/cglC	1979853..1980179 (-)	327	WP_050260822.1	comG operon protein ComGC	Machinery gene
AAA985_RS10445 (TKY181970_20370)	comGB/cglB	1980181..1981197 (-)	1017	WP_073177425.1	competence type IV pilus assembly protein ComGB	Machinery gene
AAA985_RS10450 (TKY181970_20380)	comGA/cglA/cilD	1981145..1982086 (-)	942	WP_000249559.1	competence type IV pilus ATPase ComGA	Machinery gene
AAA985_RS10455 (TKY181970_20390)	-	1982162..1982527 (-)	366	WP_000286415.1	DUF1033 family protein	-
AAA985_RS10460 (TKY181970_20400)	-	1982794..1984049 (+)	1256	WP_088804233.1	ISL3 family transposase	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17850.40 Da
• Isoelectric Point: 8.0016

>NTDB_id=105998 AAA985_RS10425 WP_000250540.1 1978767..1979228(-) (comGF/cglF) [Streptococcus pneumoniae strain Pne2]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEKS

Nucleotide

• Length: 462 bp

>NTDB_id=105998 AAA985_RS10425 WP_000250540.1 1978767..1979228(-) (comGF/cglF) [Streptococcus pneumoniae strain Pne2]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTACTTGCCCTCAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAACTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (45-141)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae Rx1	94.771	100	0.948
comGF/cglF	Streptococcus pneumoniae D39	94.771	100	0.948
comGF/cglF	Streptococcus pneumoniae R6	94.771	100	0.948
comGF/cglF	Streptococcus pneumoniae TIGR4	94.771	100	0.948
comGF/cglF	Streptococcus mitis SK321	93.464	100	0.935
comGF/cglF	Streptococcus mitis NCTC 12261	92.157	100	0.922