Detailed information NTG1059076

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: ACFE3T_RS01945
• Genome accession: NZ_CP170742
• Coordinates: 398211..399917 (-)
• Length: 568 a.a.
• NCBI ID: WP_058394588.1
• Uniprot ID: -
• Organism: Aeromonas salmonicida strain XN-7
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IScluster/Tn	400039..401270	398211..399917	flank	122

Gene organization within MGE regions

Location: 398211..401270

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFE3T_RS01945 (ACFE3T_01945)	pilB	398211..399917 (-)	1707	WP_058394588.1	PilB family type IVa pilus assembly ATPase TapB	Machinery gene
ACFE3T_RS01950 (ACFE3T_01950)	-	400066..401270 (+)	1205	WP_100654772.1	IS3 family transposase	-

Sequence

Protein

• Length: 568 a.a.
• Molecular weight: 62546.97 Da
• Isoelectric Point: 5.4384

>NTDB_id=1059076 ACFE3T_RS01945 WP_058394588.1 398211..399917(-) (pilB) [Aeromonas salmonicida strain XN-7]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESEQDALGMEEIDESEI
SELEVSDENSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRVSGLEKARLGVTSLA
EINRVTTN

Nucleotide

• Length: 1707 bp

>NTDB_id=1059076 ACFE3T_RS01945 WP_058394588.1 398211..399917(-) (pilB) [Aeromonas salmonicida strain XN-7]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCATCCCTGCTCAGTGAGTCCGACTCACAGCGTTA
CCTGAGCCAAGCCAAGGCCCAGCGCAAACCCTTCGTGACATTTCTGATTGAAAACGAGATCCTCGACAGCAAGGCGCTGG
CGGATTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAACTGATTGAGAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTGTCGGCACTGGAGGATTTCGGTTTCAGCTTCGGGCTCCATACCGAAGCCCTGCTGGTGGAAGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAAAGCGAGCAGGATGCCCTCGGCATGGAGGAGATCGACGAGTCGGAGATC
TCCGAGCTGGAAGTCTCGGACGAAAACTCCCGCCTCGATGAGAGCGTCAATACCGCCGACGACGATGCCCCCATCGTCAA
ATACATCAACAAAATCATGATGGATGCCATCAAGCGCGGCGCCTCGGATCTGCACTTCGAACCCTATGAGACCAAGTACC
GTATCCGCTTTCGTATAGACGGCATACTGCACGAAATAGCGACGCCGCCAGTCAATCTGGCCAACCGCTTTTCGGCCCGC
CTCAAGGTGATGGCGCGACTCGATATCGCAGAGCGACGGCTGCCCCAGGATGGTCGCATCAAGTTAAAGCTGTCGCGCAA
CAAATCCATGGATATGCGGGTCAACACACTGCCCACCATGTGGGGCGAGAAGATCGTTATCCGTCTGCTGGACTCTTCCG
CAGCGCGGCTCAATATCGAACAGCTCGGCTTCGACGACCGGCAGAAGGCACAGTATCTGCGTGCCCTCTCCAAGCCACAG
GGCATGATATTGGTGACAGGCCCGACCGGCTCTGGCAAGACGGTCTCTCTCTATACCGGCCTCAATATTCTCAACACCAC
AGAGGTCAACATCTCTACCGCCGAAGACCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTACAAGTCAATCCCAAGG
CGGGGCTGACCTTCGCCAGCGCCCTGCGCTCCTTTCTTCGCCAGGATCCCGACGTGGTGATGGTGGGGGAAATCCGGGAT
CTGGAAACCGCAGAGATCGCCATCAAAGCCGCCCAAACCGGCCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCTGC
CGAAACCCTGACCCGCATGATGAACATGGGGGTCCCGGCCTTCAATATTGCCTCTTCGGTAACACTCATCATGGCCCAGC
GCCTCGCTCGCAAGCTCTGCGATCACTGCAAGGCGCCGGAAGTGGTGCCCGAGGCTGAACTGCTGGAGCTGGGCTTCACC
CAGCAGCAGTTGGCCGCCGGCTTGCGCCTGTTCAAGCCAGTAGGCTGCAAAGAGTGCTCGGGGGGCTACAAGGGTCGGGT
CGGTATCTACGAAATCATGCTAATGTCTGAAAACATTGCAAAATTGATCATGCAAGGAGCCAACTCCCTGCAAATTGCAG
CGATTGCTCAAAAAGAGGGGATGCGAACCCTGCGTGTCTCGGGCCTGGAGAAGGCCCGCCTGGGGGTGACCAGCCTGGCA
GAGATCAACCGGGTGACCACCAACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (191-458)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (42-147)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baylyi ADP1	55.614	100	0.558
pilB	Acinetobacter baumannii D1279779	55.556	99.824	0.555
pilB	Legionella pneumophila strain ERS1305867	54.594	99.648	0.544
pilB	Vibrio cholerae strain A1552	54.513	99.472	0.542
pilB	Vibrio campbellii strain DS40M4	52.12	99.648	0.519
pilB	Vibrio parahaemolyticus RIMD 2210633	51.596	99.296	0.512
pilF	Neisseria gonorrhoeae MS11	46.454	99.296	0.461
pilB/pilB1	Synechocystis sp. PCC 6803	38.879	97.359	0.379
pilF	Thermus thermophilus HB27	40.341	92.958	0.375