Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACFCQI_RS12330 Genome accession   NZ_CP170722
Coordinates   2759283..2760563 (-) Length   426 a.a.
NCBI ID   WP_395117093.1    Uniprot ID   A0AB74UX84
Organism   Rhodanobacter sp. FW102-FHT14D06     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2755515..2772138 2759283..2760563 within 0


Gene organization within MGE regions


Location: 2755515..2772138
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFCQI_RS12300 (ACFCQI_12300) - 2755515..2756330 (+) 816 WP_395117081.1 thymidylate synthase -
  ACFCQI_RS12305 (ACFCQI_12305) - 2756422..2756910 (+) 489 WP_395117083.1 dihydrofolate reductase -
  ACFCQI_RS12310 (ACFCQI_12310) - 2756910..2757308 (+) 399 WP_395117085.1 NUDIX domain-containing protein -
  ACFCQI_RS12315 (ACFCQI_12315) coaE 2757328..2757939 (-) 612 WP_395117087.1 dephospho-CoA kinase -
  ACFCQI_RS12320 (ACFCQI_12320) pilD 2757936..2758793 (-) 858 WP_395117089.1 prepilin peptidase Machinery gene
  ACFCQI_RS12325 (ACFCQI_12325) - 2758804..2759196 (-) 393 WP_395117091.1 endonuclease domain-containing protein -
  ACFCQI_RS12330 (ACFCQI_12330) pilC 2759283..2760563 (-) 1281 WP_395117093.1 type II secretion system F family protein Machinery gene
  ACFCQI_RS12335 (ACFCQI_12335) pilB 2760705..2762426 (-) 1722 WP_395117095.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACFCQI_RS12340 (ACFCQI_12340) - 2762672..2762911 (+) 240 WP_395117097.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
  ACFCQI_RS12345 (ACFCQI_12345) - 2762908..2763303 (+) 396 WP_395117099.1 PIN domain-containing protein -
  ACFCQI_RS12350 (ACFCQI_12350) - 2763372..2764229 (+) 858 WP_395117101.1 glycosyltransferase family 2 protein -
  ACFCQI_RS12355 (ACFCQI_12355) gmd 2764273..2765367 (+) 1095 WP_395117103.1 GDP-mannose 4,6-dehydratase -
  ACFCQI_RS12360 (ACFCQI_12360) - 2765372..2766880 (+) 1509 WP_395117105.1 lipopolysaccharide biosynthesis protein -
  ACFCQI_RS12365 (ACFCQI_12365) - 2766899..2767750 (+) 852 WP_395117107.1 hypothetical protein -
  ACFCQI_RS12370 (ACFCQI_12370) - 2767750..2768775 (+) 1026 WP_395117109.1 glycosyltransferase family 2 protein -
  ACFCQI_RS12375 (ACFCQI_12375) - 2768775..2769734 (+) 960 WP_395117110.1 GDP-mannose 4,6-dehydratase -
  ACFCQI_RS12380 (ACFCQI_12380) - 2769731..2770984 (+) 1254 WP_395117112.1 class I SAM-dependent methyltransferase -
  ACFCQI_RS12385 (ACFCQI_12385) - 2770984..2772138 (+) 1155 WP_395117114.1 glycoside hydrolase family 99-like domain-containing protein -

Sequence

Protein


Download         Length: 426 a.a.        Molecular weight: 46246.32 Da        Isoelectric Point: 9.8296

>NTDB_id=1058893 ACFCQI_RS12330 WP_395117093.1 2759283..2760563(-) (pilC) [Rhodanobacter sp. FW102-FHT14D06]
MATATATAKARADKTRALGAQRAEVSQLTTYEWVALDKRGKRMKGDMPAKNASLVKAELRRQGMNPQTVRERAKPLFGAS
GSTIKPGDVAIFSRQIATMMASGVPMVQSFDIIADGQKNVRFKNVLLDVKQNIEGGASLHEALGRYPVQFDELYCNLVHA
GETSGVLDTVLDTVATYKERTEAIKKKIKKALFYPMMVLAVVFIVILIMLLFVVPVFAKTFQDAGAQLPAPTQFLVDASE
FMQGYWYIVVGIIVGSIAAIVIGKKRSPKFAHFLDRLALKMPVMGDIVRNSAIARFARTLGVTFRAGVPLVEALDAVAGA
TGSIVYGDAVRQMRDDISVGHQLQLAMKQTGLFPNMVVQMTGIGEESGSLDSMLFKVAEFYEEEVSNAVDTLSTLLEPLI
MVVLGTLVGGIVIALYLPIFKLAGTF

Nucleotide


Download         Length: 1281 bp        

>NTDB_id=1058893 ACFCQI_RS12330 WP_395117093.1 2759283..2760563(-) (pilC) [Rhodanobacter sp. FW102-FHT14D06]
ATGGCCACGGCTACCGCAACCGCAAAAGCAAGGGCAGACAAGACGCGTGCGCTCGGCGCCCAGCGGGCCGAGGTCAGTCA
ACTGACCACTTACGAATGGGTGGCGCTGGACAAGCGCGGCAAGCGCATGAAGGGCGACATGCCCGCCAAGAACGCCTCGC
TGGTCAAGGCCGAGTTGCGCCGCCAGGGCATGAACCCGCAAACCGTGCGCGAACGGGCCAAGCCGCTGTTCGGCGCCTCC
GGCAGCACGATCAAGCCGGGCGACGTGGCGATCTTCAGCCGCCAGATCGCCACCATGATGGCTTCCGGCGTGCCGATGGT
GCAGTCCTTCGACATCATCGCCGACGGTCAGAAGAACGTCCGCTTCAAGAACGTGCTGCTCGACGTCAAGCAGAACATCG
AGGGCGGCGCCTCGCTGCACGAGGCGCTGGGCCGCTACCCGGTGCAGTTCGACGAGCTGTACTGCAACCTGGTGCATGCC
GGCGAAACCTCGGGCGTGCTGGACACCGTGCTGGACACGGTGGCCACCTACAAGGAACGCACCGAGGCGATCAAGAAGAA
GATCAAGAAGGCGCTGTTCTACCCGATGATGGTGCTGGCGGTGGTGTTCATCGTCATCCTGATCATGCTGCTGTTCGTGG
TGCCGGTGTTCGCCAAGACCTTCCAGGACGCGGGCGCGCAGTTGCCCGCGCCGACCCAGTTCCTGGTCGACGCCTCGGAG
TTCATGCAGGGCTACTGGTACATCGTGGTGGGCATCATCGTCGGCAGCATCGCCGCCATCGTCATCGGCAAGAAGCGCTC
GCCGAAGTTCGCCCACTTCCTGGATCGCCTCGCGCTGAAAATGCCCGTGATGGGCGACATCGTGCGCAACTCCGCGATCG
CCCGCTTCGCTCGCACGCTGGGCGTCACCTTCCGCGCCGGCGTGCCGCTGGTGGAAGCCCTGGATGCCGTGGCCGGTGCC
ACCGGCAGCATCGTCTACGGCGACGCCGTGCGACAGATGCGCGACGACATCTCGGTCGGCCACCAGTTGCAGCTGGCCAT
GAAACAGACCGGGCTGTTCCCCAACATGGTGGTACAGATGACCGGCATCGGCGAGGAGTCCGGCTCGCTGGACAGCATGC
TGTTCAAAGTCGCCGAGTTCTACGAGGAAGAGGTCAGCAACGCGGTGGACACCCTCTCCACCCTGCTCGAACCCCTCATC
ATGGTGGTGCTGGGCACCCTGGTCGGCGGCATCGTGATCGCGCTGTACCTGCCGATCTTCAAGCTGGCTGGCACGTTCTG
A

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(92-215)

Type II secretion system (T2SS), protein F (T2SSF)

(296-418)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.046

92.488

0.491
  pilC Legionella pneumophila strain ERS1305867

52.911

92.723

0.491
  pilC Acinetobacter baylyi ADP1

48.768

95.305

0.465
  pilC Acinetobacter baumannii D1279779

49.747

92.958

0.462
  pilG Neisseria meningitidis 44/76-A

42.67

89.671

0.383
  pilG Neisseria gonorrhoeae MS11

42.67

89.671

0.383

Multiple sequence alignment