Detailed information NTG1058485

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACFXAD_RS08285
• Genome accession: NZ_CP170653
• Coordinates: 1617537..1618151 (+)
• Length: 204 a.a.
• NCBI ID: WP_053573180.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain JP5011
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1612537..1623151

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFXAD_RS08250 (ACFXAD_08250)	aroE	1613224..1614066 (+)	843	WP_039254404.1	shikimate dehydrogenase	-
ACFXAD_RS08255 (ACFXAD_08255)	yhbY	1614060..1614350 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ACFXAD_RS08260 (ACFXAD_08260)	-	1614361..1614930 (+)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
ACFXAD_RS08265 (ACFXAD_08265)	yqeK	1614920..1615480 (+)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACFXAD_RS08270 (ACFXAD_08270)	rsfS	1615498..1615854 (+)	357	WP_007408260.1	ribosome silencing factor	-
ACFXAD_RS08275 (ACFXAD_08275)	-	1615851..1616588 (+)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
ACFXAD_RS08280 (ACFXAD_08280)	comER	1616657..1617478 (-)	822	WP_015417855.1	late competence protein ComER	-
ACFXAD_RS08285 (ACFXAD_08285)	comEA	1617537..1618151 (+)	615	WP_053573180.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACFXAD_RS08290 (ACFXAD_08290)	-	1618218..1618787 (+)	570	WP_003152868.1	ComE operon protein 2	-
ACFXAD_RS08295 (ACFXAD_08295)	comEC	1618788..1621139 (+)	2352	WP_053573181.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACFXAD_RS08300 (ACFXAD_08300)	-	1621158..1621292 (-)	135	WP_003152870.1	YqzM family protein	-
ACFXAD_RS08305 (ACFXAD_08305)	-	1621333..1621487 (+)	155	Protein_1586	hypothetical protein	-
ACFXAD_RS08310 (ACFXAD_08310)	holA	1621527..1622568 (+)	1042	Protein_1587	DNA polymerase III subunit delta	-
ACFXAD_RS08315 (ACFXAD_08315)	rpsT	1622585..1622851 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22183.34 Da
• Isoelectric Point: 6.4888

>NTDB_id=1058485 ACFXAD_RS08285 WP_053573180.1 1617537..1618151(+) (comEA) [Bacillus amyloliquefaciens strain JP5011]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTMVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1058485 ACFXAD_RS08285 WP_053573180.1 1617537..1618151(+) (comEA) [Bacillus amyloliquefaciens strain JP5011]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTTGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATGGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.328	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363