Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACFJIT_RS10065 Genome accession   NZ_CP170559
Coordinates   2303071..2304795 (+) Length   574 a.a.
NCBI ID   WP_130561825.1    Uniprot ID   -
Organism   Pseudoxanthomonas sp. UC19_8     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2298071..2309795
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFJIT_RS10050 (ACFJIT_10050) - 2298881..2300509 (+) 1629 WP_130561828.1 PAS domain-containing sensor histidine kinase -
  ACFJIT_RS10055 (ACFJIT_10055) pilR 2300601..2302064 (+) 1464 WP_130561827.1 sigma-54 dependent transcriptional regulator Regulator
  ACFJIT_RS10060 (ACFJIT_10060) pilA2 2302416..2302862 (+) 447 WP_130561826.1 pilin Machinery gene
  ACFJIT_RS10065 (ACFJIT_10065) pilB 2303071..2304795 (+) 1725 WP_130561825.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACFJIT_RS10070 (ACFJIT_10070) pilC 2304937..2306199 (+) 1263 WP_130561824.1 type II secretion system F family protein Machinery gene
  ACFJIT_RS10075 (ACFJIT_10075) - 2306203..2307066 (+) 864 WP_130561823.1 A24 family peptidase -
  ACFJIT_RS10080 (ACFJIT_10080) - 2307304..2308308 (-) 1005 WP_130561822.1 class I fructose-bisphosphate aldolase -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62496.76 Da        Isoelectric Point: 5.7965

>NTDB_id=1058022 ACFJIT_RS10065 WP_130561825.1 2303071..2304795(+) (pilB) [Pseudoxanthomonas sp. UC19_8]
MNAIASPNLVGITGIARRLVMDGALEEGAARTAMAEASHAKIPLAQWIQEMKLVSPAQLAAANAIEFGIPLIDASAFDPA
QNALKLVSEELLQKHQILPMFKRGNKLYVGVSDPTRMQGLDEIKFHTNLAVEPILVADDQLRRTLETWSNASDSLAGMGG
DDEDFGSLDTAAGDEESGDTGIDAKGEDTPVVKFVNKMLVDAIRKGASDIHFEPYETDYRVRLRIDGILKTVARAPQKLN
QRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTRQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEEDQQKLFLD
AIHKPYGMVLVTGPTGSGKTVSLYTALGILNDDTRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDIIM
VGEIRDLETAEIAVKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCPRCKRSATIPAKAL
LAEGFSQAELDEGFTVFEAVGCDECTEGYKGRVGIYQVMPMTDEIATIILEGGGAIQIAEAAQRSGIRDLRQSALWKVKQ
GLTSLAEINRVTKD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=1058022 ACFJIT_RS10065 WP_130561825.1 2303071..2304795(+) (pilB) [Pseudoxanthomonas sp. UC19_8]
ATGAACGCAATTGCATCTCCTAACCTTGTCGGCATTACTGGAATCGCCCGCCGCCTCGTGATGGATGGCGCGCTTGAAGA
AGGCGCCGCTCGCACCGCGATGGCCGAAGCATCTCATGCCAAGATTCCATTGGCGCAGTGGATACAAGAGATGAAGCTTG
TAAGCCCTGCCCAGCTTGCTGCTGCCAACGCCATCGAGTTTGGCATTCCTTTGATTGATGCGTCAGCGTTCGACCCTGCT
CAAAACGCTCTGAAATTGGTCAGCGAGGAACTGCTGCAAAAGCACCAAATCCTGCCCATGTTCAAGCGTGGCAACAAGCT
GTACGTCGGGGTCTCCGACCCGACGCGCATGCAAGGTCTCGACGAAATTAAGTTCCATACGAACCTTGCTGTGGAGCCGA
TTCTGGTCGCTGATGACCAGCTTAGAAGGACGCTGGAAACATGGAGTAATGCCTCCGACTCGCTCGCCGGGATGGGTGGC
GACGACGAGGATTTCGGCAGCCTGGACACGGCGGCAGGCGACGAGGAGAGCGGCGACACAGGCATCGACGCCAAGGGTGA
GGATACGCCAGTCGTTAAGTTCGTCAACAAAATGCTGGTCGATGCCATCCGCAAGGGGGCTTCTGACATTCATTTTGAGC
CATACGAGACCGACTACCGTGTGCGCTTACGCATCGACGGCATCCTCAAGACCGTCGCGCGGGCCCCGCAGAAGCTCAAC
CAGCGCATCGCCGCGCGCCTGAAGGTCATGTCGCAGCTGGACATCGCCGAAAAGCGCGTTCCGCAAGACGGCCGCATCAA
GCTCAACCTATCCAAGACCCGCCAGATCGACTTCCGTGTCAGCACCCTGCCGACGCTGTTCGGCGAGAAGATCGTGCTGC
GTATCCTGGATGGCAGCGCCGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGGAGGACCAGCAGAAGCTGTTCCTGGAT
GCCATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGCTCCGGCAAAACCGTGTCGCTGTATACGGCGCT
GGGCATCCTCAACGACGACACGCGCAACATCTCCACGGCCGAGGACCCGGTCGAAATCCGCCTGCCCGGCGTCAACCAGG
TCCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGCCAGGACCCGGACATCATCATG
GTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATCGCCGTCAAGGCCGCCCAGACCGGCCACATGGTGCTCTCGACCCT
GCACACCAACGATGCACCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCCTACAACATCACCAGTTCGGTGA
CCCTGGTCATCGCGCAGCGCCTGGCGCGCCGCCTGTGCCCACGCTGCAAGCGATCCGCCACGATCCCGGCCAAGGCGCTG
CTGGCCGAGGGGTTCAGCCAAGCAGAGCTGGACGAGGGGTTTACCGTCTTCGAGGCGGTGGGTTGCGACGAGTGCACTGA
AGGCTACAAAGGACGCGTGGGCATCTACCAGGTCATGCCGATGACCGATGAGATCGCCACGATCATCCTGGAGGGCGGTG
GCGCCATTCAGATCGCCGAGGCCGCGCAGCGTTCGGGCATCCGTGACCTGCGCCAGTCGGCCCTATGGAAGGTCAAGCAG
GGGCTGACCAGCCTGGCCGAGATCAACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(197-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.14

99.303

0.557
  pilB Acinetobacter baylyi ADP1

53.571

100

0.549
  pilB Legionella pneumophila strain ERS1305867

52.12

98.606

0.514
  pilB Vibrio cholerae strain A1552

50

98.606

0.493
  pilF Neisseria gonorrhoeae MS11

51.238

91.463

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

49.24

91.638

0.451
  pilB Vibrio campbellii strain DS40M4

49.315

89.024

0.439
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.36

92.509

0.364

Multiple sequence alignment