Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACFJI0_RS02895 Genome accession   NZ_CP170552
Coordinates   548232..549965 (-) Length   577 a.a.
NCBI ID   WP_382161347.1    Uniprot ID   -
Organism   Hydrogenophaga sp. UC242_53     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 543232..554965
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFJI0_RS02865 (ACFJI0_02865) - 544004..544456 (+) 453 WP_382323372.1 NUDIX domain-containing protein -
  ACFJI0_RS02870 (ACFJI0_02870) - 544487..544690 (-) 204 WP_382323373.1 DNA gyrase inhibitor YacG -
  ACFJI0_RS02875 (ACFJI0_02875) zapD 544699..545455 (-) 757 Protein_573 cell division protein ZapD -
  ACFJI0_RS02880 (ACFJI0_02880) coaE 545499..546113 (-) 615 WP_382323374.1 dephospho-CoA kinase -
  ACFJI0_RS02885 (ACFJI0_02885) - 546110..546973 (-) 864 WP_382323375.1 prepilin peptidase -
  ACFJI0_RS02890 (ACFJI0_02890) pilC 546973..548190 (-) 1218 WP_291121454.1 type II secretion system F family protein Machinery gene
  ACFJI0_RS02895 (ACFJI0_02895) pilB 548232..549965 (-) 1734 WP_382161347.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACFJI0_RS02905 (ACFJI0_02905) - 550252..551181 (-) 930 WP_382325896.1 polyprenyl synthetase family protein -
  ACFJI0_RS02910 (ACFJI0_02910) rplU 551446..551757 (+) 312 WP_382161335.1 50S ribosomal protein L21 -
  ACFJI0_RS02915 (ACFJI0_02915) rpmA 551771..552028 (+) 258 WP_297397423.1 50S ribosomal protein L27 -
  ACFJI0_RS02920 (ACFJI0_02920) cgtA 552169..553242 (+) 1074 WP_382323376.1 Obg family GTPase CgtA -
  ACFJI0_RS02925 (ACFJI0_02925) proB 553312..554442 (+) 1131 WP_382325898.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62657.09 Da        Isoelectric Point: 5.6281

>NTDB_id=1057940 ACFJI0_RS02895 WP_382161347.1 548232..549965(-) (pilB) [Hydrogenophaga sp. UC242_53]
MASVETVPQETPSMALPGLGRALVSAGKLGQKAAEDLYRKAQSGRSSFIAELTGSGAVSASDLAHTMSVAFAAPLLDLDA
IDVQRLPAGLLDPKICSDYRIVVLSKRNNRLMVATADPADQQAAEKIKFATQMGVDWVIAEYDKLSKLVETQTTSVNDTM
DNIIGGEFEFDEAATETSVSDVTDKASEVDDAPVVKFLHKMLIDAFNMRASDLHFEPYEHNYRVRFRVDGELREIASPPI
VIKDKLASRIKVISRMDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKVGIDALGYEPEEKER
LLNAISRPYGMVLVTGPTGSGKTVSLYTCLNILNKPGINISTAEDPSEINLPGVNQVNVNEKAGLTFAAALKAFLRQDPD
VIMVGEIRDLETADISIKAAQTGHMVLSTLHTNDAPTTLTRMMNMGIPTFNIASSVILITAQRLARRLCATCKAPLDVPR
KALVDAGFKPEDLDGTWTPYKPVGCSACNNGYKGRVGIYQVMPISEEIQRIILRGGSALDIAQQAGKEGVRTLRESGLLK
VKLGMTSLEEVLSVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1057940 ACFJI0_RS02895 WP_382161347.1 548232..549965(-) (pilB) [Hydrogenophaga sp. UC242_53]
ATGGCGTCCGTCGAAACCGTCCCCCAGGAGACCCCGTCCATGGCCTTGCCAGGCCTGGGCCGCGCGCTCGTGTCTGCGGG
GAAGCTTGGCCAGAAGGCGGCTGAAGATCTTTATCGCAAAGCACAAAGCGGCCGCTCCAGCTTCATCGCAGAGCTGACCG
GCTCAGGGGCTGTGTCCGCTTCGGATCTGGCCCACACCATGTCGGTGGCGTTTGCCGCGCCACTGCTCGATCTGGACGCG
ATCGACGTGCAACGCCTGCCCGCCGGCCTGCTCGATCCCAAGATCTGCAGCGACTACCGCATCGTCGTCCTGAGCAAACG
CAACAACCGCTTGATGGTCGCGACGGCCGATCCAGCCGACCAACAGGCCGCCGAGAAGATCAAGTTCGCGACCCAGATGG
GCGTGGACTGGGTGATTGCCGAGTACGACAAGCTCAGCAAACTGGTGGAGACCCAGACCACCAGCGTCAACGACACGATG
GACAACATCATCGGTGGCGAGTTCGAGTTCGACGAGGCGGCCACTGAGACGAGCGTGAGCGACGTGACCGACAAGGCGTC
GGAGGTCGACGACGCGCCCGTGGTCAAGTTCCTGCACAAGATGCTGATTGACGCATTCAACATGCGGGCATCCGACTTGC
ACTTTGAGCCTTACGAGCACAACTACCGGGTCCGTTTTCGGGTGGACGGCGAACTGCGTGAAATTGCGTCGCCGCCGATC
GTGATCAAGGACAAGCTGGCGTCGCGGATCAAGGTGATCTCCCGCATGGACATCTCGGAGAAGCGTGTGCCCCAGGACGG
GCGCATGAAACTCAAGATCGGACCGGACCGCGTGATCGACTTTCGGGTCAGCACCCTGCCCACCTTGTTCGGCGAGAAGA
TCGTGATCCGTATTCTGGACCCGAGCAGCGCCAAGGTCGGCATCGATGCCCTGGGCTACGAACCCGAGGAAAAAGAGCGG
CTGTTGAACGCGATCAGCCGGCCATACGGCATGGTGCTGGTCACCGGCCCCACGGGGTCGGGGAAAACGGTGTCGCTGTA
CACCTGCCTGAACATCCTGAACAAGCCAGGCATCAACATTTCCACGGCGGAAGATCCGTCGGAAATCAACCTGCCGGGTG
TCAATCAGGTCAACGTCAACGAGAAGGCGGGCCTGACCTTCGCGGCGGCCCTCAAGGCATTTCTGCGCCAGGATCCCGAC
GTGATCATGGTGGGTGAGATTCGCGATCTCGAAACGGCGGACATCTCGATCAAGGCCGCACAAACCGGTCACATGGTGCT
GTCCACCCTGCACACGAACGACGCGCCGACCACACTGACGCGGATGATGAACATGGGCATTCCCACGTTCAACATCGCCT
CCAGCGTGATCCTGATCACCGCCCAGCGGCTGGCCCGTCGCCTGTGCGCCACCTGCAAGGCGCCGCTGGACGTACCGCGC
AAGGCCCTGGTGGACGCCGGATTCAAGCCGGAAGACCTTGATGGCACCTGGACGCCCTACAAGCCCGTGGGGTGCTCGGC
CTGCAACAACGGCTACAAGGGCCGCGTCGGCATCTACCAGGTGATGCCCATTTCGGAAGAAATCCAACGCATCATCCTGC
GTGGTGGCAGCGCGCTCGACATTGCCCAGCAGGCCGGCAAGGAGGGCGTTCGCACGCTGCGCGAATCGGGTCTGCTCAAG
GTGAAGCTGGGCATGACTTCGCTGGAAGAAGTGCTCAGCGTGACCAACGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.345

100

0.539
  pilB Acinetobacter baylyi ADP1

54.189

97.227

0.527
  pilF Neisseria gonorrhoeae MS11

52.827

98.094

0.518
  pilB Legionella pneumophila strain ERS1305867

49.737

98.96

0.492
  pilB Vibrio parahaemolyticus RIMD 2210633

45.714

97.054

0.444
  pilB Vibrio cholerae strain A1552

48.369

90.295

0.437
  pilB Vibrio campbellii strain DS40M4

43.915

98.267

0.432
  pilF Thermus thermophilus HB27

37.961

98.614

0.374
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.696

99.307

0.374

Multiple sequence alignment