Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACFJY4_RS19105 Genome accession   NZ_CP170500
Coordinates   4035750..4037900 (-) Length   716 a.a.
NCBI ID   WP_020307761.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain MBI-RS3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4030750..4042900
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFJY4_RS19090 (ACFJY4_19085) - 4032463..4034061 (-) 1599 WP_386413698.1 AAA family ATPase -
  ACFJY4_RS19095 (ACFJY4_19090) aroB 4034072..4035175 (-) 1104 WP_020307763.1 3-dehydroquinate synthase -
  ACFJY4_RS19100 (ACFJY4_19095) aroK 4035199..4035744 (-) 546 WP_020307762.1 shikimate kinase AroK -
  ACFJY4_RS19105 (ACFJY4_19100) pilQ 4035750..4037900 (-) 2151 WP_020307761.1 type IV pilus secretin PilQ Machinery gene
  ACFJY4_RS19110 (ACFJY4_19105) pilP 4037954..4038481 (-) 528 WP_014595707.1 type 4a pilus biogenesis lipoprotein PilP -
  ACFJY4_RS19115 (ACFJY4_19110) pilO 4038478..4039104 (-) 627 WP_020307760.1 type 4a pilus biogenesis protein PilO -
  ACFJY4_RS19120 (ACFJY4_19115) pilN 4039101..4039673 (-) 573 WP_386413702.1 type 4a pilus biogenesis protein PilN -
  ACFJY4_RS19125 (ACFJY4_19120) comM 4039673..4040737 (-) 1065 WP_340065837.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 716 a.a.        Molecular weight: 76935.04 Da        Isoelectric Point: 4.7456

>NTDB_id=1057790 ACFJY4_RS19105 WP_020307761.1 4035750..4037900(-) (pilQ) [Stutzerimonas stutzeri strain MBI-RS3]
MNTTLSRLGVSLLAAFISPALLAANLNALDVAALPGDRVELKLAFDEPVASPRGYTLDQPARIALDLPGVSNNLGAKNRE
LGVGNARSVTVVEAQGRTRLIVNLTALVPYSTRVDGNNLFVVLGESSGTTSPAAASAAVTAPIAKSAPVKPMAAGKAIDN
IDFRRGEDGAGNVVITLSDPSVGSTIEEQGGKIRVVFAKAELPEALRVRLDVQDFATPVKFVDSTAQAGGASIAIEPTGR
YDYLAYQTDNILTISVKPLTEDEVDKRKAERFSYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVNGNITLRLQNV
PWDQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESQRQIAELAPLRREVVQVNYAKAADIARLFQSVADNGAI
AEDRGSIAVDDRTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGNLWAGDKWNTWGRDG
KLGVTDDPNASGKPGDEGYIPSRQVGRFPGIRETPLNTPFVDLGAVGATSGFGLGFVTDNAILDLQLSAMEKTGNGEVVS
QPKVVTADKETAKILKGAEVPYQEASSSGATSTSFKEAALSLEVTPQITPDDRIIMEVRVTKDEPDFSQAASTGGVPSIR
KNEVNAKVLINDGETIVIGGVFSNTQSKSVDKVPFLGDLPFVGRVFRRDLVQDRKSELLVFLTPRIMNNQAIQASR

Nucleotide


Download         Length: 2151 bp        

>NTDB_id=1057790 ACFJY4_RS19105 WP_020307761.1 4035750..4037900(-) (pilQ) [Stutzerimonas stutzeri strain MBI-RS3]
ATGAACACTACCCTTTCTCGCCTGGGCGTATCTCTGCTGGCGGCATTTATATCGCCAGCACTGCTGGCGGCCAATCTCAA
CGCCTTGGATGTGGCTGCTTTGCCGGGCGATCGGGTTGAGTTGAAACTGGCATTTGACGAGCCTGTTGCGTCACCTCGTG
GCTACACGCTGGATCAGCCTGCACGCATTGCTCTGGATCTTCCAGGCGTATCCAACAACCTGGGGGCCAAAAATCGGGAG
CTGGGCGTTGGTAATGCGCGCAGCGTAACGGTCGTCGAGGCGCAAGGGCGCACCCGGCTCATCGTCAATCTGACCGCGCT
TGTTCCGTATTCCACTCGAGTGGATGGGAACAATCTGTTTGTCGTGCTGGGAGAGAGCTCGGGGACGACCAGCCCTGCGG
CTGCATCCGCCGCGGTCACTGCTCCGATTGCCAAGTCTGCTCCGGTAAAGCCGATGGCGGCCGGCAAGGCGATCGACAAT
ATCGATTTTCGCCGCGGTGAGGATGGTGCCGGAAACGTGGTAATCACACTGTCCGATCCGTCGGTAGGTTCGACTATCGA
AGAGCAGGGAGGAAAGATCCGCGTTGTGTTCGCCAAGGCGGAGCTGCCTGAAGCACTGCGTGTTCGGCTCGACGTTCAGG
ATTTCGCTACGCCGGTGAAGTTTGTCGACTCGACCGCTCAGGCAGGCGGCGCCAGTATCGCCATCGAGCCTACTGGGCGA
TACGATTATCTCGCGTATCAAACGGATAATATATTGACTATAAGCGTCAAACCTCTTACGGAAGACGAAGTTGATAAGCG
CAAGGCTGAGCGTTTTTCCTACTCAGGTGAAAAACTGTCGCTTAACTTTCAAGACATCGACGTTCGCTCTGTGCTTCAGC
TTATTGCCGACTTTACCGATCTCAATCTGGTGGCAAGTGATACTGTAAATGGCAATATCACCCTTCGTTTGCAAAATGTG
CCTTGGGATCAGGCGCTAGATTTAGTTCTAAAGACCAAGGGGCTGGATAAGCGCCAAGTTGGGAATGTACTGCTCGTAGC
TCCCGCTGATGAAATCGCCGCGCGTGAACGCCAAGAGCTTGAGTCGCAGCGCCAGATCGCCGAACTTGCTCCGCTGCGTC
GCGAGGTTGTGCAGGTGAACTATGCCAAGGCTGCAGACATTGCTCGCCTGTTTCAATCGGTCGCAGACAATGGTGCTATT
GCGGAGGATCGCGGCTCTATCGCGGTCGATGATCGTACCAATAATATTATCGCTTACCAGACTCAGGATCGACTTGATGA
ATTGCGACGCATCGTTGCTCAGCTCGACATTCCGGTGCGGCAAGTGATGATTGAGGCTCGTATAGTTGAAGCTAACGTTG
ATTATGACAAAAGTTTAGGGGTTCGTTGGGGCGGTAATCTTTGGGCCGGTGATAAGTGGAACACTTGGGGGCGGGATGGA
AAGTTAGGTGTCACAGATGATCCTAATGCTTCCGGAAAACCCGGCGACGAAGGATACATTCCCTCTCGTCAGGTTGGTCG
CTTTCCCGGCATACGTGAGACCCCGCTCAATACTCCATTTGTCGACCTAGGGGCCGTTGGAGCTACGTCCGGGTTCGGGT
TGGGTTTTGTTACCGATAATGCAATTCTTGATCTTCAGTTGTCGGCGATGGAAAAGACCGGTAATGGAGAAGTTGTATCT
CAGCCGAAAGTGGTGACCGCTGATAAGGAAACAGCAAAAATACTAAAGGGCGCCGAGGTGCCGTATCAAGAAGCTAGTTC
GAGTGGTGCCACTAGCACCTCTTTTAAAGAGGCAGCTCTTTCGCTTGAAGTGACCCCACAGATTACTCCAGATGATCGAA
TCATTATGGAGGTAAGGGTTACTAAGGATGAGCCGGACTTTTCTCAGGCTGCAAGCACGGGTGGCGTCCCGTCTATTAGA
AAAAATGAGGTTAATGCTAAAGTGTTAATAAACGATGGTGAGACGATTGTCATCGGTGGCGTATTCTCTAACACCCAGAG
TAAGTCGGTAGATAAGGTTCCTTTCTTGGGGGATCTCCCATTTGTGGGGCGCGTATTCCGTCGTGATCTAGTGCAGGATC
GAAAATCCGAACTGCTAGTGTTCTTAACCCCTAGAATCATGAATAATCAGGCGATCCAAGCCAGTCGCTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(375-441)

Bacterial type II and III secretion system protein (Secretin)

(549-707)

Secretin and TonB N terminus short domain (STN)

(302-348)

AMIN domain (AMIN)

(38-123)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.055

100

0.735
  comQ Acinetobacter baylyi ADP1

40.756

99.721

0.406
  pilQ Acinetobacter baumannii strain A118

38.804

100

0.39
  pilQ Acinetobacter baumannii D1279779

38.804

100

0.39

Multiple sequence alignment