Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACFJY4_RS17225 Genome accession   NZ_CP170500
Coordinates   3652988..3654691 (-) Length   567 a.a.
NCBI ID   WP_386413420.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain MBI-RS3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3647988..3659691
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFJY4_RS17195 (ACFJY4_17190) - 3648074..3648754 (+) 681 WP_013981946.1 energy-coupling factor ABC transporter permease -
  ACFJY4_RS17200 (ACFJY4_17195) yacG 3648767..3648964 (-) 198 WP_041771656.1 DNA gyrase inhibitor YacG -
  ACFJY4_RS17205 (ACFJY4_17200) coaE 3648961..3649569 (-) 609 WP_080505042.1 dephospho-CoA kinase -
  ACFJY4_RS17210 (ACFJY4_17205) - 3649851..3650831 (-) 981 WP_058131641.1 IS5-like element ISPa16 family transposase -
  ACFJY4_RS17215 (ACFJY4_17210) pilD 3650897..3651766 (-) 870 WP_017246372.1 A24 family peptidase Machinery gene
  ACFJY4_RS17220 (ACFJY4_17215) pilC 3651769..3652986 (-) 1218 WP_351157777.1 type II secretion system F family protein Machinery gene
  ACFJY4_RS17225 (ACFJY4_17220) pilB 3652988..3654691 (-) 1704 WP_386413420.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACFJY4_RS17230 (ACFJY4_17225) pilA 3655003..3655479 (+) 477 WP_090519242.1 pilin Machinery gene
  ACFJY4_RS17235 (ACFJY4_17230) - 3656608..3657172 (+) 565 Protein_3382 transposase -
  ACFJY4_RS17240 (ACFJY4_17235) - 3657404..3657844 (+) 441 WP_386413423.1 YtoQ family protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 61780.76 Da        Isoelectric Point: 5.3409

>NTDB_id=1057785 ACFJY4_RS17225 WP_386413420.1 3652988..3654691(-) (pilB) [Stutzerimonas stutzeri strain MBI-RS3]
MSENISLSGLARQMVVAGVLDEKTAQQAQAQAVRGKTPFVTYVVQNKLAKGRAIAELASDQFGVALLDLSALNKESLPKD
LISEKLARQHRVLPLSKRGTKLYVAISDPANHQSIADIQFSTGLSIESILVEDDKLGEAIDKLYESGATGLEELGEVDLD
GVDVESVTEDGKAEEGGEAADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKAYRVRLRTDGILHEVARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEDQKALYMQALQQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVAAFNIATSVNLIIAQRLARKLCNSCKKEVELPHDALLEEGFP
ADKIGTFKIYGPVGCDNCKGGYKGRVGIYEVVKNTPALARIIMEDGNSIDISTQMRKDGFNDLRTSALLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=1057785 ACFJY4_RS17225 WP_386413420.1 3652988..3654691(-) (pilB) [Stutzerimonas stutzeri strain MBI-RS3]
ATGAGCGAAAACATTTCCCTATCCGGGCTGGCACGGCAGATGGTCGTGGCCGGCGTGTTGGACGAAAAAACGGCCCAGCA
GGCGCAGGCTCAAGCCGTTCGCGGCAAAACACCCTTTGTCACCTATGTAGTGCAGAACAAGTTGGCCAAAGGCCGCGCCA
TAGCCGAGCTTGCTTCCGATCAATTCGGTGTAGCGCTGCTGGACCTGTCTGCGCTGAACAAGGAGTCGCTGCCCAAGGAT
CTGATCAGTGAGAAGTTGGCCCGCCAACATCGGGTGCTGCCGCTTTCCAAGCGCGGCACCAAGCTTTATGTCGCCATCTC
CGATCCGGCTAATCATCAATCGATTGCCGATATCCAGTTCAGTACCGGGCTGAGCATCGAGTCGATTCTGGTGGAAGACG
ACAAGCTGGGCGAGGCCATCGACAAACTGTACGAAAGCGGCGCCACCGGACTAGAGGAGCTGGGCGAAGTCGATCTGGAT
GGGGTCGATGTCGAGTCCGTCACCGAAGATGGAAAAGCTGAGGAAGGCGGAGAGGCTGCTGACGACGCCCCTGTAGTGCG
CTTCGTCAACAAGATGCTGCTCGACGCCATTCGTGGTGGCTCGTCCGATTTGCACTTCGAGCCCTACGAAAAAGCCTACC
GCGTGCGGCTTCGCACGGATGGCATCCTGCATGAGGTCGCGCGCCCACCGATCCAACTGGCGCCCCGCATATCTGCACGC
TTGAAGGTGATGGCCGGGCTGGATATCTCCGAGCGACGCAAGCCGCAGGACGGGCGGATCAAGATGAAGCTGTCCAAGAC
CAAGGCCATCGACTTTCGGGTGAACACGCTGCCGACGCTGTGGGGCGAGAAGATCGTGATGCGGATTCTCGATCCATCCA
GTGCACAGATGGGCATCGATGCGTTGGGGTATGAGGAAGATCAGAAGGCGCTGTATATGCAGGCGCTGCAACAGCCGCAG
GGCATGATCCTGGTGACCGGCCCTACCGGGTCGGGCAAGACGGTCTCCCTTTATACCGGCCTGAACATCCTTAATACGGT
GGATGTGAATATTTCCACAGCGGAAGATCCGGTGGAGATAAACCTCGAGGGTATCAACCAGGTCAACGTCAATCCTCGGC
AGGGCATGGACTTCTCTCAGGCGCTGCGGGCGTTTCTGCGCCAGGACCCGGACATCATCATGGTGGGGGAAATCCGAGAC
CTGGAAACCGCCGAAATTGCCATCAAGGCCGCGCAGACCGGCCATATGGTGATGTCGACGCTGCACACCAACAGCGCCGC
GGAAACCCTGACGCGCCTGCGCAACATGGGCGTGGCGGCGTTCAATATCGCCACCTCGGTCAACCTGATCATTGCCCAGC
GCCTGGCACGCAAGCTTTGCAACTCGTGCAAGAAAGAAGTGGAACTGCCGCACGATGCCCTATTGGAAGAAGGCTTCCCT
GCCGACAAGATCGGTACGTTCAAAATCTACGGCCCGGTGGGCTGTGATAATTGCAAAGGCGGTTACAAAGGTCGTGTTGG
TATTTATGAAGTGGTTAAAAACACGCCAGCGTTGGCCAGGATTATCATGGAAGACGGTAATTCGATCGATATTTCCACCC
AGATGCGAAAAGACGGCTTCAATGACCTGCGTACCTCGGCTTTGCTCAAGGCCATGCAGGGCGTGACCAGCCTGGAAGAA
GTCAACCGGGTGACCAAGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.574

99.471

0.593
  pilB Acinetobacter baylyi ADP1

59.325

99.295

0.589
  pilB Legionella pneumophila strain ERS1305867

54.737

100

0.55
  pilB Vibrio campbellii strain DS40M4

48.596

100

0.489
  pilF Neisseria gonorrhoeae MS11

48.845

99.295

0.485
  pilB Vibrio cholerae strain A1552

52.437

90.476

0.474
  pilB Vibrio parahaemolyticus RIMD 2210633

52.233

90.829

0.474
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.083

96.12

0.376

Multiple sequence alignment