Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACEQUB_RS14170 Genome accession   NZ_CP170451
Coordinates   2873943..2875670 (+) Length   575 a.a.
NCBI ID   WP_197333427.1    Uniprot ID   -
Organism   Ralstonia syzygii strain Br19     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2868943..2880670
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEQUB_RS14155 (ACEQUB_02875) - 2869670..2870281 (+) 612 WP_197333430.1 hypothetical protein -
  ACEQUB_RS14160 - 2871013..2871492 (-) 480 WP_390623048.1 site-specific integrase -
  ACEQUB_RS14165 (ACEQUB_02877) - 2872418..2873695 (+) 1278 WP_197333428.1 HlyC/CorC family transporter -
  ACEQUB_RS14170 (ACEQUB_02878) pilB 2873943..2875670 (+) 1728 WP_197333427.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACEQUB_RS14175 (ACEQUB_02879) pilC 2875714..2876979 (+) 1266 WP_197333426.1 type II secretion system F family protein Machinery gene
  ACEQUB_RS14180 (ACEQUB_02880) - 2876982..2877863 (+) 882 WP_269437001.1 A24 family peptidase -
  ACEQUB_RS14185 (ACEQUB_02881) coaE 2877876..2878490 (+) 615 WP_197333425.1 dephospho-CoA kinase -
  ACEQUB_RS14190 zapD 2878607..2879366 (+) 760 Protein_2788 cell division protein ZapD -
  ACEQUB_RS14195 (ACEQUB_02884) - 2879378..2879593 (+) 216 WP_197333423.1 DNA gyrase inhibitor YacG -
  ACEQUB_RS14200 (ACEQUB_02885) - 2879586..2880047 (-) 462 WP_197333422.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63688.48 Da        Isoelectric Point: 7.4489

>NTDB_id=1057520 ACEQUB_RS14170 WP_197333427.1 2873943..2875670(+) (pilB) [Ralstonia syzygii strain Br19]
MTLGLALAQSRRIAPTLLTQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDTVPAVLSANKHF
QQLRLLPLGRRDNRIIVATSDPSDPKPIEELRQKMNVAIETVIVEHDKLVRQLSLANEAPTEIKSLIPKQEATQIEYDPG
AAATVRRTTTDGIDDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILAQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDKNDKDNKETIERAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNKLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKACKKPGPLPRETL
LDAGFKEADLDGSWQPYHPVGCEACNGSGYKGRVGIYQVMPITEAIQEIILSHGTALQIAEQARKEGVLSLRDSGLRKVK
QGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=1057520 ACEQUB_RS14170 WP_197333427.1 2873943..2875670(+) (pilB) [Ralstonia syzygii strain Br19]
ATGACTCTCGGACTCGCTCTTGCGCAGAGCCGGCGAATTGCGCCGACATTACTCACCCAGCTTGAACAGAGCGCGCGCGA
AAAGCGCACGCAGCTGATCGATGAACTGGTGAGCAGCGGCATCATGAGCGCGCACGATCTCGCCGTCTTCGTGGCGGGCA
AGTACCAGATGCCGCTGCTGGACCTGGCGCAGTACAAGCTCGATACCGTGCCGGCCGTGCTGTCGGCTAACAAGCATTTC
CAGCAATTGCGCCTGTTGCCGCTGGGCCGGCGCGACAACCGCATCATCGTTGCCACCTCGGACCCGAGCGACCCCAAGCC
GATCGAGGAACTGCGCCAGAAGATGAACGTGGCGATCGAGACGGTCATCGTCGAGCACGACAAGCTGGTGCGCCAGCTGA
GCCTCGCCAACGAAGCGCCGACCGAGATCAAGTCGCTCATCCCCAAGCAGGAAGCCACGCAGATCGAATACGACCCGGGC
GCAGCCGCTACCGTGCGGCGCACCACGACCGACGGCATCGACGATGCGCCGGTGGTGCGTTTCCTGCAAAAGCTGTTTAC
CGAGGCGCTGCTGCGCGGCACCTCCGACTTGCATTTCGAGCCGTTTGAAACGTTTTACCGCGTGCGCTTCCGTATCGACG
GCATCCTCGCGCAGGTCGCGCAGCCACCGCTGGATATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTGTCGCGCCTG
GACATCTCGGAAAAGCGCGTGCCGCAAGACGGCCGCATGAAGCTCCTGATCACGGTTCCGAAAGACAAGAACGACAAGGA
CAACAAGGAGACGATCGAGCGCGCCATCGACTTCCGGGTGTCGACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATCC
GGATTCTGGAGTCCTCCACCGAGCGGCTCGATATCGACCAGCTAGGCTATGAGCCCGAGCAAAAGCAGCTCCTGCTGGAC
GTGATCAAGCGCCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGTAGCGGCAAGACGGTCTCGCTGTACACCTTCCT
GAACAAGCTGAACCAGGGCGACATCAACATTTCCACCGCGGAAGACCCGGCGGAAATCCAGTTGCCCGGCATCAACCAGG
TCAACGTCAACGACAAAGCGGGGCTGACCTTCGCCGCGGCGCTGAAATCGTTCCTGCGGCAGGATCCGGACATCATCATG
GTGGGCGAAATCCGGGACCTGGAAACCGCCGACATCTCCATCAAGGCCGCGCAGACCGGCCACTTGGTGTTCTCCACGCT
GCACACCAACGATGCGCCGACCACGCTCACGCGGCTGATGAACATGGGCGTGGCACCGTTCAACATCGCATCGTCGGTGC
TGATGATCACGGCGCAGCGTCTGGGGCGGCGCCTGTGCAAGGCATGCAAGAAGCCGGGCCCGCTGCCCCGGGAAACATTG
CTGGATGCCGGCTTCAAGGAAGCCGACCTGGACGGCTCGTGGCAGCCGTACCATCCAGTCGGCTGCGAGGCATGCAACGG
CAGCGGCTACAAGGGCCGGGTCGGCATCTACCAGGTCATGCCGATCACCGAGGCGATCCAGGAGATCATCCTCAGCCACG
GCACGGCACTGCAGATCGCCGAACAGGCGCGCAAGGAAGGCGTGCTCTCGCTGCGCGATTCTGGCCTGCGGAAAGTGAAA
CAGGGGCTGACTTCCCTTGAGGAAGTGCTGGCCACGACGAACCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(184-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

50.973

98.261

0.501
  pilF Neisseria gonorrhoeae MS11

53.148

93.913

0.499
  pilB Acinetobacter baylyi ADP1

49.911

97.913

0.489
  pilB Legionella pneumophila strain ERS1305867

46.585

99.304

0.463
  pilB Vibrio cholerae strain A1552

44.604

96.696

0.431
  pilB Vibrio campbellii strain DS40M4

44.834

94.261

0.423
  pilB Vibrio parahaemolyticus RIMD 2210633

44.465

94.261

0.419
  pilB/pilB1 Synechocystis sp. PCC 6803

35.596

100

0.374
  pilF Thermus thermophilus HB27

37.699

98.261

0.37

Multiple sequence alignment