Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACFIOZ_RS10055 Genome accession   NZ_CP170448
Coordinates   2225798..2227546 (-) Length   582 a.a.
NCBI ID   WP_386083670.1    Uniprot ID   -
Organism   Vreelandella sp. F11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2220798..2232546
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFIOZ_RS10025 (ACFIOZ_10025) - 2221435..2221803 (+) 369 WP_386083657.1 hypothetical protein -
  ACFIOZ_RS10030 (ACFIOZ_10030) - 2221831..2222718 (-) 888 WP_386083660.1 DNA replication terminus site-binding protein -
  ACFIOZ_RS10035 (ACFIOZ_10035) yacG 2222758..2222991 (-) 234 WP_022523595.1 DNA gyrase inhibitor YacG -
  ACFIOZ_RS10040 (ACFIOZ_10040) coaE 2223026..2223628 (-) 603 WP_386083664.1 dephospho-CoA kinase -
  ACFIOZ_RS10045 (ACFIOZ_10045) - 2223698..2224543 (-) 846 WP_386083666.1 prepilin peptidase -
  ACFIOZ_RS10050 (ACFIOZ_10050) pilC 2224562..2225794 (-) 1233 WP_386083669.1 type II secretion system F family protein Machinery gene
  ACFIOZ_RS10055 (ACFIOZ_10055) pilB 2225798..2227546 (-) 1749 WP_386083670.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACFIOZ_RS10060 (ACFIOZ_10060) - 2227787..2228281 (+) 495 WP_386083672.1 pilin -
  ACFIOZ_RS10065 (ACFIOZ_10065) - 2228415..2228849 (+) 435 WP_386083674.1 anti-virulence regulator CigR family protein -
  ACFIOZ_RS10070 (ACFIOZ_10070) - 2228944..2229765 (+) 822 WP_386083676.1 inositol monophosphatase family protein -
  ACFIOZ_RS10080 (ACFIOZ_10080) - 2230696..2231439 (+) 744 WP_386083677.1 Crp/Fnr family transcriptional regulator -
  ACFIOZ_RS10085 (ACFIOZ_10085) - 2231856..2232272 (+) 417 WP_386083679.1 DUF488 domain-containing protein -

Sequence

Protein


Download         Length: 582 a.a.        Molecular weight: 63793.37 Da        Isoelectric Point: 6.7500

>NTDB_id=1057495 ACFIOZ_RS10055 WP_386083670.1 2225798..2227546(-) (pilB) [Vreelandella sp. F11]
MSQPPFESTLSHAAARGGLHGFAQRLVKHGLLTEAQAATAETTALDLEISLLQHVIESGLVDANKATLAAGWEYGLPVID
LEAVRLSALPSAADYPIKVLQSLNVLPLAHHGHRLTVAVPYPATLTQLDELQFATGLSVEGILAPVDQIMVALNSYLAQN
GRGMLDELDDAVSSLSIVQNNEGDELPLEESSQSSDDAPIVKFVNKILLDAIRRGASDIHFEPYETLYRIRFRIDGMLIE
VARPPFAMRNRIAARLKIMARLDISERRLPQDGSIKLKLSRTRSLDFRVNSLPTVYGEKLVLRILDPTAAQLGIEQLGFT
DEQRSHYEHALKQPQGMILVTGPTGSGKTVSLYTGINILNKVERNICTAEDPVEIKVAGINQVNVLPKIGLDFVSALRAF
LRQDPDVVMVGEIRDLETAEIAVKAAQTGHLVLSTVHTNSAAETLTRLANMGVSSFNIASAVSLIIAQRLARKLCQHCKE
PINIPHEALRKEGFSSAEIQKATIYKPVGCKHCTHGYKGRIGIYEVVPISEAMRQLIMRDANALDIDQQRRQEGYPSLHR
SGLLKVMQGITSLEEVNRVSKE

Nucleotide


Download         Length: 1749 bp        

>NTDB_id=1057495 ACFIOZ_RS10055 WP_386083670.1 2225798..2227546(-) (pilB) [Vreelandella sp. F11]
ATGAGCCAGCCCCCTTTTGAGTCGACGTTAAGCCACGCCGCTGCACGTGGCGGCCTGCACGGCTTTGCCCAGCGCTTGGT
TAAGCACGGCCTGCTGACCGAGGCCCAGGCCGCCACAGCGGAAACCACCGCGTTGGATCTGGAGATATCGCTTCTTCAGC
ATGTGATCGAAAGCGGCCTGGTCGATGCTAACAAAGCCACACTGGCAGCGGGCTGGGAGTATGGGCTGCCAGTCATAGAT
CTGGAAGCAGTTCGACTTTCCGCGCTGCCTTCTGCTGCTGACTACCCTATCAAAGTGCTGCAGAGCCTTAACGTTCTGCC
ATTAGCCCACCACGGCCATCGTCTAACGGTTGCAGTTCCCTACCCCGCCACGCTGACGCAACTGGATGAGTTGCAGTTTG
CGACCGGCCTGAGCGTGGAAGGCATCCTAGCTCCCGTCGATCAAATCATGGTCGCGCTCAATAGCTACCTTGCTCAAAAC
GGGCGTGGCATGCTTGATGAGTTAGATGATGCGGTGAGTTCACTGAGCATTGTGCAGAATAACGAGGGTGACGAACTTCC
TCTGGAAGAGTCTTCTCAGAGTAGTGACGACGCCCCCATTGTTAAGTTCGTCAACAAGATATTGTTAGACGCGATCCGCC
GGGGCGCCTCTGATATTCATTTTGAGCCTTACGAAACCCTCTACCGGATACGCTTTCGTATTGATGGAATGCTCATAGAA
GTGGCGCGACCACCCTTTGCCATGCGCAATCGTATCGCGGCCCGCCTAAAAATCATGGCTCGACTGGATATCTCGGAACG
CCGCTTACCCCAGGATGGCTCGATAAAGTTAAAGCTATCGCGCACCCGCTCTCTCGATTTCCGGGTCAACTCATTGCCAA
CCGTTTATGGCGAGAAGTTAGTGCTGCGGATATTAGATCCGACCGCGGCGCAACTGGGTATTGAGCAATTAGGCTTTACC
GACGAACAACGGAGCCATTATGAGCATGCGTTAAAACAACCCCAAGGCATGATTCTGGTCACTGGCCCTACCGGAAGCGG
CAAGACCGTTTCGTTATATACCGGCATTAATATTCTTAATAAAGTTGAGCGTAATATTTGTACCGCTGAAGACCCGGTTG
AGATCAAAGTCGCCGGTATCAATCAAGTGAACGTATTACCTAAGATCGGACTCGATTTTGTCAGTGCACTAAGGGCATTT
TTACGGCAAGACCCGGATGTGGTGATGGTAGGCGAAATTCGCGACCTTGAAACCGCCGAAATTGCCGTTAAAGCAGCCCA
GACAGGGCACTTGGTGCTCTCCACGGTGCATACCAACTCGGCAGCTGAAACGCTGACTCGACTGGCGAATATGGGCGTCT
CTTCTTTTAACATCGCCAGCGCCGTTAGTTTGATTATTGCCCAGCGACTGGCTCGCAAACTGTGTCAACACTGCAAAGAG
CCCATTAACATTCCCCATGAAGCGCTGCGTAAAGAAGGCTTTAGCAGCGCAGAGATCCAAAAAGCGACTATCTACAAACC
GGTAGGTTGCAAGCACTGTACCCACGGCTATAAAGGGCGAATTGGCATTTATGAGGTAGTACCGATTAGTGAAGCGATGC
GTCAGCTAATTATGCGTGATGCCAACGCATTAGATATCGACCAGCAGAGGCGCCAGGAGGGCTACCCGAGTCTGCACCGT
AGTGGGTTGCTGAAAGTCATGCAGGGTATTACCAGCCTGGAAGAGGTTAACCGTGTGAGCAAGGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(206-473)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-159)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

51.59

97.251

0.502
  pilB Acinetobacter baylyi ADP1

50.176

97.595

0.49
  pilB Acinetobacter baumannii D1279779

50

96.907

0.485
  pilB Vibrio cholerae strain A1552

49.819

94.845

0.473
  pilB Vibrio campbellii strain DS40M4

48.393

96.22

0.466
  pilB Vibrio parahaemolyticus RIMD 2210633

48.043

96.564

0.464
  pilF Neisseria gonorrhoeae MS11

49.896

82.646

0.412

Multiple sequence alignment