Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BCU67_RS04345 Genome accession   NZ_CP170019
Coordinates   867093..868778 (-) Length   561 a.a.
NCBI ID   WP_016790036.1    Uniprot ID   -
Organism   Vibrio cyclitrophicus strain 10N.286.49.B11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 862093..873778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCU67_RS04315 (BCU67_04315) rplS 862614..862967 (+) 354 WP_004735514.1 50S ribosomal protein L19 -
  BCU67_RS04320 (BCU67_04320) yacG 863249..863446 (-) 198 WP_010435916.1 DNA gyrase inhibitor YacG -
  BCU67_RS04325 (BCU67_04325) zapD 863528..864268 (-) 741 WP_010435917.1 cell division protein ZapD -
  BCU67_RS04330 (BCU67_04330) coaE 864295..864909 (-) 615 WP_010435920.1 dephospho-CoA kinase -
  BCU67_RS04335 (BCU67_04335) pilD 864912..865781 (-) 870 WP_102277664.1 prepilin peptidase Machinery gene
  BCU67_RS04340 (BCU67_04340) pilC 865848..867077 (-) 1230 WP_016791284.1 type II secretion system F family protein Machinery gene
  BCU67_RS04345 (BCU67_04345) pilB 867093..868778 (-) 1686 WP_016790036.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BCU67_RS04350 (BCU67_04350) pilA 868779..869198 (-) 420 WP_016790035.1 pilin Machinery gene
  BCU67_RS04355 (BCU67_04355) nadC 869453..870343 (-) 891 WP_016790034.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BCU67_RS04360 (BCU67_04360) ampD 870427..871025 (+) 599 Protein_757 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BCU67_RS04365 (BCU67_04365) pdhR 871507..872268 (+) 762 WP_016768209.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62044.81 Da        Isoelectric Point: 5.2073

>NTDB_id=1054653 BCU67_RS04345 WP_016790036.1 867093..868778(-) (pilB) [Vibrio cyclitrophicus strain 10N.286.49.B11]
MLTNLPTVLRQAGLLSLAQEQAVAEQVQASGISTPEALLVLDFFSGDSLANNIKTIFGLPLVQLANTDYETLCDQLGLRE
LITKYRAIPIAVSSSTLTLASADPTDLQAEDDFRFATGLQIELVVANYSELEGAIRKLYGRSISGQDSKRKEITQDELAN
LVKVSDDEITSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEEHYRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEAQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTSERNISTAEDPVEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDIVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNRCKTADDSPDIYLRHSIPNSQT
IYKANSQGCNECNQGYSGRVGIYEVMQFTDKLKTSLINKPNALAIEDLARREGMRTLQESGLDKLLEGTTSYQELQRVLY
I

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1054653 BCU67_RS04345 WP_016790036.1 867093..868778(-) (pilB) [Vibrio cyclitrophicus strain 10N.286.49.B11]
GTGCTAACCAACCTCCCAACCGTTCTACGTCAGGCTGGCTTACTTAGCCTGGCTCAAGAACAAGCCGTTGCGGAACAAGT
ACAAGCTTCAGGTATTTCGACACCTGAAGCTTTGCTCGTTCTAGATTTCTTCAGCGGCGATTCCTTAGCAAACAACATTA
AAACCATCTTCGGCTTACCGTTAGTACAACTTGCCAATACGGACTATGAAACCTTATGTGACCAATTAGGGCTTCGCGAA
CTGATTACTAAATATCGTGCTATCCCGATTGCAGTTTCTAGCTCCACCCTCACACTCGCTTCTGCCGACCCTACGGATTT
ACAAGCTGAAGATGACTTCCGCTTTGCGACAGGATTACAGATTGAATTGGTTGTCGCTAATTACTCTGAATTAGAAGGCG
CAATACGCAAGCTGTATGGTCGATCTATTTCCGGGCAAGATTCTAAGCGTAAAGAGATCACCCAAGATGAGCTAGCTAAC
CTCGTGAAGGTATCTGACGACGAAATAACGTCGATTGAAGATCTCAGCCAAGACGACTCCCCTGTGAGTCGCTTTATCAA
TCAGATTCTGGTTGATGCGGTACGTAAAGGCGCATCGGACATCCATTTTGAGCCCTACGAAGAACACTACCGAGTGCGCC
TACGTTGCGATGGCATTCTTGTCGAGATCCAACAACCCGCCTCTCATTTAAGCCGTCGATTATCAGCGCGCTTAAAGATC
CTCGCAAAACTGGATATTGCAGAACGTCGCTTACCTCAAGATGGTCGTATTAAACTGCGTTTAAATGACGAACTGGCAAT
TGATATGCGCGTATCAACACTGCCTACCTTATGGGGCGAGAAAATCGTACTGCGATTGCTTGATAGCAGTGCGGCCAATC
TCGATATCGATAAGCTCGGTTACAGCGAGGCTCAAAAAGCCCTCTACCTCAATGCACTAAAGCGCCCGCAAGGGATGATC
TTAATGACCGGGCCAACCGGCAGCGGTAAAACGGTTTCTCTTTATACTGGGCTTCGAGTGCTCAACACCTCTGAACGCAA
CATCTCAACAGCTGAAGACCCAGTAGAGATTAACCTGTGTGGTATCAATCAGGTACAAGTGACACCTAAGATTGGCTTTG
GTTTCGCCGAGGCACTACGATCTTTTTTACGACAAGATCCAGACATCGTGATGGTTGGAGAAATCCGAGATTTAGAAACC
GCTGAAATCGCGATCAAAGCATCGCAAACAGGTCACTTGGTGCTTTCTACACTGCACACCAATTCTGCTGCTGAAACCGT
AACTCGGCTGGCTCATATGGGAATTGAACCCTTTAATCTTGCTTCGTCTTTAAGCTTGATTATTGCTCAGCGTCTTGCGA
GACGCTTGTGTAACCGCTGTAAAACCGCAGACGACTCACCAGACATTTATCTGCGCCACTCTATCCCAAACAGCCAAACC
ATCTACAAAGCCAACTCTCAAGGCTGTAATGAGTGTAACCAAGGTTACTCAGGTCGAGTGGGGATCTATGAAGTGATGCA
GTTTACCGACAAGCTAAAAACCAGCCTTATCAACAAACCCAATGCGCTAGCGATTGAAGATCTCGCGCGTCGAGAAGGCA
TGCGCACTCTGCAAGAGTCAGGTCTGGATAAATTGCTTGAAGGCACCACCAGCTATCAAGAGCTGCAACGTGTTCTGTAC
ATATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(11-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.936

100

0.743
  pilB Vibrio campbellii strain DS40M4

70.819

100

0.709
  pilB Vibrio parahaemolyticus RIMD 2210633

70.641

100

0.708
  pilB Acinetobacter baumannii D1279779

50.674

92.513

0.469
  pilB Legionella pneumophila strain ERS1305867

50.392

90.909

0.458
  pilB Acinetobacter baylyi ADP1

49.419

91.979

0.455
  pilF Neisseria gonorrhoeae MS11

42.908

100

0.431
  pilF Thermus thermophilus HB27

37.77

99.109

0.374

Multiple sequence alignment