Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BCV09_RS02280 Genome accession   NZ_CP170013
Coordinates   444877..446562 (-) Length   561 a.a.
NCBI ID   WP_029189239.1    Uniprot ID   -
Organism   Vibrio cyclitrophicus strain 10N.286.54.C7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 439877..451562
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCV09_RS02250 (BCV09_02250) rplS 440402..440755 (+) 354 WP_004735514.1 50S ribosomal protein L19 -
  BCV09_RS02255 (BCV09_02255) yacG 441034..441231 (-) 198 WP_010435916.1 DNA gyrase inhibitor YacG -
  BCV09_RS02260 (BCV09_02260) zapD 441313..442053 (-) 741 WP_010435917.1 cell division protein ZapD -
  BCV09_RS02265 (BCV09_02265) coaE 442080..442694 (-) 615 WP_010435920.1 dephospho-CoA kinase -
  BCV09_RS02270 (BCV09_02270) pilD 442697..443566 (-) 870 WP_016768203.1 prepilin peptidase Machinery gene
  BCV09_RS02275 (BCV09_02275) pilC 443633..444862 (-) 1230 WP_016768204.1 type II secretion system F family protein Machinery gene
  BCV09_RS02280 (BCV09_02280) pilB 444877..446562 (-) 1686 WP_029189239.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BCV09_RS02285 (BCV09_02285) pilA 446567..446992 (-) 426 WP_016796129.1 pilin Machinery gene
  BCV09_RS02290 (BCV09_02290) nadC 447247..448137 (-) 891 WP_102402180.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BCV09_RS02295 (BCV09_02295) ampD 448221..448820 (+) 600 WP_065107040.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BCV09_RS02300 (BCV09_02300) pdhR 449302..450063 (+) 762 WP_016768209.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62110.74 Da        Isoelectric Point: 5.1241

>NTDB_id=1054510 BCV09_RS02280 WP_029189239.1 444877..446562(-) (pilB) [Vibrio cyclitrophicus strain 10N.286.54.C7]
MLTNLPTVLRQADLLSLTQEQAVAEHVQASGISTPEALLVLDFFSGDSLANNIKTIFGLPLVRLASTDYESLCEQLGLRE
LITKYRAIPISVSSSTLTLASADPTDLQAEDDFRFATGLQVELVVANYSELEGAIRKLYGRSISGQDSKRKEITQDELAN
LVKVSDDEITSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEEHYRVRLRCDGILVETQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEAQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTSERNISTAEDPVEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDIVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKETDDSPDIFLRHSIPNSQT
IYKANSQGCNECNQGYSGRVGIYEVMPFTDNLKTSLINKPNALAIEDLARREGMRTLQESGLDKLLEGTTSYQELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1054510 BCV09_RS02280 WP_029189239.1 444877..446562(-) (pilB) [Vibrio cyclitrophicus strain 10N.286.54.C7]
GTGCTAACCAACCTCCCAACCGTTCTACGTCAGGCTGATTTACTTAGCCTGACTCAGGAACAAGCCGTGGCGGAACATGT
ACAAGCTTCAGGTATTTCGACACCTGAAGCTTTACTCGTTCTGGATTTTTTCAGCGGCGATTCCTTAGCAAACAACATTA
AAACCATCTTCGGCTTACCGTTAGTACGATTAGCCAGTACTGACTACGAATCCCTGTGTGAACAATTAGGGCTTCGTGAA
CTGATTACTAAGTATCGTGCTATTCCAATTTCAGTTTCTAGCTCAACTCTCACACTCGCTTCTGCCGACCCTACGGATTT
ACAGGCTGAAGATGACTTCCGCTTTGCGACAGGGTTGCAGGTTGAATTGGTTGTTGCTAATTACTCTGAATTAGAAGGCG
CGATACGCAAGCTGTATGGTCGATCTATTTCCGGGCAAGATTCTAAGCGTAAAGAGATCACCCAAGATGAGCTAGCTAAC
CTCGTGAAGGTATCTGACGACGAAATAACGTCGATTGAAGATCTCAGCCAAGACGACTCCCCTGTGAGTCGCTTTATCAA
TCAGATTCTGGTTGATGCGGTACGTAAAGGCGCATCGGACATCCATTTTGAGCCCTACGAAGAACACTACCGAGTGCGCC
TACGTTGCGATGGCATTCTTGTCGAGACCCAACAGCCCGCCTCTCATTTAAGCCGTCGATTATCAGCGCGCTTAAAGATC
CTCGCAAAACTGGATATTGCAGAACGTCGCTTACCTCAAGATGGTCGTATTAAACTGCGTTTAAATGACGAACTGGCAAT
TGATATGCGCGTATCAACACTGCCTACCTTATGGGGCGAGAAGATCGTACTGCGATTGCTTGATAGCAGTGCGGCCAATC
TCGATATCGATAAGCTCGGTTACAGCGAGGCGCAAAAAGCCCTCTACCTCAATGCACTAAAGCGCCCGCAAGGGATGATC
TTAATGACCGGGCCAACCGGCAGCGGTAAAACGGTTTCTCTTTATACTGGACTTCGAGTACTCAACACCTCTGAACGCAA
CATCTCAACAGCTGAAGACCCTGTAGAGATTAACCTGTGTGGCATCAATCAGGTACAAGTGACGCCTAAGATTGGCTTTG
GTTTCGCCGAGGCACTACGATCGTTTTTACGACAAGACCCAGACATCGTGATGGTTGGAGAAATCCGAGATTTAGAAACC
GCCGAGATCGCGATCAAAGCATCGCAAACAGGTCACTTGGTGCTTTCTACACTGCACACCAATTCTGCTGCTGAAACCGT
AACTCGGCTTGCTCACATGGGAATTGAACCCTTTAATCTTGCTTCGTCATTGAGCCTAATCATAGCCCAACGACTGGCAA
GACGCTTGTGCAACCATTGCAAAGAAACAGACGACTCGCCGGACATATTTCTGCGTCACTCGATTCCAAACAGCCAAACC
ATCTACAAAGCCAACTCTCAAGGCTGTAATGAGTGTAACCAAGGTTACTCAGGTCGAGTGGGTATCTATGAAGTGATGCC
GTTTACCGACAACCTAAAAACCAGCCTTATCAATAAACCCAATGCGCTAGCGATTGAAGATCTCGCACGTCGAGAAGGCA
TGCGCACTCTGCAAGAGTCAGGGCTAGATAAACTGCTTGAAGGCACCACCAGCTATCAAGAGCTACAACGTGTTCTATAC
CTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

74.113

100

0.745
  pilB Vibrio parahaemolyticus RIMD 2210633

70.819

100

0.709
  pilB Vibrio campbellii strain DS40M4

70.641

100

0.708
  pilB Legionella pneumophila strain ERS1305867

48.007

98.396

0.472
  pilB Acinetobacter baumannii D1279779

50.674

92.513

0.469
  pilB Acinetobacter baylyi ADP1

49.419

91.979

0.455
  pilF Neisseria gonorrhoeae MS11

44.324

98.93

0.438
  pilF Thermus thermophilus HB27

39.155

92.87

0.364

Multiple sequence alignment