Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BCV24_RS02280 Genome accession   NZ_CP170005
Coordinates   444857..446542 (-) Length   561 a.a.
NCBI ID   WP_016787826.1    Uniprot ID   A0A7Z1S0U0
Organism   Vibrio cyclitrophicus strain 10N.286.54.F9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 439857..451542
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCV24_RS02250 (BCV24_02250) rplS 440354..440707 (+) 354 WP_004735514.1 50S ribosomal protein L19 -
  BCV24_RS02255 (BCV24_02255) yacG 441014..441211 (-) 198 WP_010435916.1 DNA gyrase inhibitor YacG -
  BCV24_RS02260 (BCV24_02260) zapD 441293..442033 (-) 741 WP_010435917.1 cell division protein ZapD -
  BCV24_RS02265 (BCV24_02265) coaE 442060..442674 (-) 615 WP_010435920.1 dephospho-CoA kinase -
  BCV24_RS02270 (BCV24_02270) pilD 442677..443546 (-) 870 WP_016768203.1 prepilin peptidase Machinery gene
  BCV24_RS02275 (BCV24_02275) pilC 443613..444842 (-) 1230 WP_016768204.1 type II secretion system F family protein Machinery gene
  BCV24_RS02280 (BCV24_02280) pilB 444857..446542 (-) 1686 WP_016787826.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BCV24_RS02285 (BCV24_02285) pilA 446542..446955 (-) 414 WP_016787825.1 pilin Machinery gene
  BCV24_RS02290 (BCV24_02290) nadC 447210..448100 (-) 891 WP_016783773.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BCV24_RS02295 (BCV24_02295) ampD 448184..448783 (+) 600 WP_016783774.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BCV24_RS02300 (BCV24_02300) pdhR 449265..450026 (+) 762 WP_016768209.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62154.89 Da        Isoelectric Point: 5.2028

>NTDB_id=1054326 BCV24_RS02280 WP_016787826.1 444857..446542(-) (pilB) [Vibrio cyclitrophicus strain 10N.286.54.F9]
MLTNLPTVLRQANLLNLTQEQAVAEHVQASGVSTPEALLVLDFFSGDSLANNIKTIFGLPLVQLANTDYESLCEQLGLRE
LITKYHAIPISVSSSTLTLASADPTDLQAEDDFRFATGLQIELVVANYSELEGAIRKLYGRSISGQDSKRKEITQDELAN
LVKVSDDEITSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEEHYRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEAQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTSERNISTAEDPVEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDIVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKETDDSPDIFLRHSIPNSQI
IYKANSQGCNECNQGYSGRVGIYEVMPFTDKLKTSLINKPNALAIEDLARREGMRTLQESGLDKLLEGTTSYQELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1054326 BCV24_RS02280 WP_016787826.1 444857..446542(-) (pilB) [Vibrio cyclitrophicus strain 10N.286.54.F9]
ATGCTCACCAACCTCCCAACCGTTCTACGTCAGGCTAACTTACTTAACCTGACTCAAGAACAAGCCGTTGCGGAACATGT
ACAAGCTTCAGGTGTTTCGACACCTGAAGCTTTGCTCGTTCTAGATTTCTTCAGCGGCGATTCCTTAGCAAACAATATAA
AAACCATCTTCGGCTTACCGTTAGTACAACTTGCCAATACGGACTATGAATCCTTGTGCGAACAATTAGGGCTTCGCGAG
CTAATCACTAAATATCACGCTATTCCGATTTCAGTTTCTAGCTCCACTCTCACACTCGCTTCTGCCGACCCTACGGATTT
ACAAGCTGAAGATGACTTCCGCTTTGCGACAGGATTACAGATTGAATTGGTTGTCGCTAATTACTCTGAATTAGAAGGCG
CAATACGCAAGCTGTATGGTCGATCTATTTCCGGGCAAGATTCTAAGCGTAAAGAGATCACCCAAGATGAGCTAGCTAAC
CTCGTGAAGGTATCTGACGACGAAATAACGTCGATTGAAGATCTCAGCCAAGACGACTCCCCTGTGAGTCGCTTTATCAA
TCAGATTCTGGTTGATGCGGTACGTAAAGGCGCATCGGACATCCATTTTGAGCCCTACGAAGAACACTACCGAGTGCGCC
TACGTTGCGATGGCATTCTTGTCGAGATCCAACAACCCGCCTCTCATTTAAGCCGTCGATTATCAGCGCGCTTAAAGATC
CTCGCAAAACTGGATATTGCAGAACGTCGCTTACCTCAAGATGGTCGTATTAAACTGCGTTTAAATGACGAACTGGCAAT
TGATATGCGCGTATCAACACTGCCTACCTTATGGGGCGAGAAGATCGTACTGCGATTGCTTGATAGCAGTGCGGCCAATC
TCGATATCGATAAGCTCGGTTACAGCGAGGCTCAAAAAGCCCTCTACCTCAATGCACTAAAGCGCCCGCAAGGGATGATC
TTAATGACCGGGCCAACCGGCAGCGGTAAAACGGTTTCTCTTTATACTGGACTTCGAGTACTCAACACCTCTGAACGCAA
CATCTCAACAGCTGAAGACCCAGTAGAGATTAACCTGTGTGGTATCAATCAGGTACAAGTGACACCTAAGATTGGCTTTG
GTTTCGCCGAGGCACTACGCTCATTTTTACGACAAGACCCAGACATCGTGATGGTTGGAGAAATCCGAGATTTAGAAACC
GCCGAAATCGCGATCAAAGCCTCCCAAACCGGGCATTTAGTACTTTCAACACTGCACACTAACTCAGCCGCAGAAACCGT
GACTCGGCTTGCTCATATGGGAATAGAGCCCTTCAACCTTGCTTCGTCATTGAGCCTAATCATAGCCCAACGACTGGCAA
GACGCTTGTGCAACCATTGCAAAGAAACAGACGACTCGCCGGACATATTTCTGCGTCACTCGATTCCTAACAGCCAAATA
ATCTACAAAGCTAACTCTCAAGGGTGTAATGAATGTAACCAAGGTTACTCAGGTCGAGTGGGTATCTATGAAGTGATGCC
GTTTACCGACAAGCTAAAAACCAGCCTTATCAACAAACCCAATGCGCTAGCGATTGAAGATCTCGCGCGTAGAGAAGGCA
TGCGCACTCTGCAAGAGTCAGGGCTAGATAAACTGCTTGAAGGCACCACCAGCTATCAAGAGCTACAACGTGTTCTATAC
CTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z1S0U0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.759

100

0.742
  pilB Vibrio campbellii strain DS40M4

70.463

100

0.706
  pilB Vibrio parahaemolyticus RIMD 2210633

70.285

100

0.704
  pilB Legionella pneumophila strain ERS1305867

47.74

98.574

0.471
  pilB Acinetobacter baumannii D1279779

50.482

92.513

0.467
  pilB Acinetobacter baylyi ADP1

49.326

92.513

0.456
  pilF Neisseria gonorrhoeae MS11

44.324

98.93

0.438
  pilF Thermus thermophilus HB27

39.347

92.87

0.365

Multiple sequence alignment