Detailed information NTG1051881

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACE1EH_RS12070
• Genome accession: NZ_CP169535
• Coordinates: 2513746..2514360 (-)
• Length: 204 a.a.
• NCBI ID: WP_375004261.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain HJ-16
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2508746..2519360

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACE1EH_RS12040 (ACE1EH_12040)	rpsT	2509049..2509315 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACE1EH_RS12045 (ACE1EH_12045)	holA	2509332..2510373 (-)	1042	Protein_2324	DNA polymerase III subunit delta	-
ACE1EH_RS12050 (ACE1EH_12050)	-	2510413..2510564 (-)	152	Protein_2325	hypothetical protein	-
ACE1EH_RS12055 (ACE1EH_12055)	-	2510605..2510739 (+)	135	WP_003152870.1	YqzM family protein	-
ACE1EH_RS12060 (ACE1EH_12060)	comEC	2510758..2513109 (-)	2352	WP_375004260.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACE1EH_RS12065 (ACE1EH_12065)	-	2513110..2513679 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACE1EH_RS12070 (ACE1EH_12070)	comEA	2513746..2514360 (-)	615	WP_375004261.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACE1EH_RS12075 (ACE1EH_12075)	comER	2514419..2515240 (+)	822	WP_375004262.1	late competence protein ComER	-
ACE1EH_RS12080 (ACE1EH_12080)	-	2515309..2516046 (-)	738	WP_032873951.1	class I SAM-dependent methyltransferase	-
ACE1EH_RS12085 (ACE1EH_12085)	rsfS	2516043..2516399 (-)	357	WP_007408260.1	ribosome silencing factor	-
ACE1EH_RS12090 (ACE1EH_12090)	yqeK	2516417..2516977 (-)	561	WP_375004263.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACE1EH_RS12095 (ACE1EH_12095)	-	2516967..2517536 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
ACE1EH_RS12100 (ACE1EH_12100)	yhbY	2517547..2517837 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ACE1EH_RS12105 (ACE1EH_12105)	aroE	2517831..2518673 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22221.42 Da
• Isoelectric Point: 6.4888

>NTDB_id=1051881 ACE1EH_RS12070 WP_375004261.1 2513746..2514360(-) (comEA) [Bacillus velezensis strain HJ-16]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVAVKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1051881 ACE1EH_RS12070 WP_375004261.1 2513746..2514360(-) (comEA) [Bacillus velezensis strain HJ-16]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGTCAAAAAAGACACCGCAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAACGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGATTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.639	93.627	0.549
comEA/celA/cilE	Streptococcus mitis NCTC 12261	36.493	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae Rx1	36.019	100	0.373
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus pneumoniae D39	36.019	100	0.373
comEA/celA/cilE	Streptococcus pneumoniae R6	36.019	100	0.373
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363