Detailed information NTG1051665

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACEX67_RS12230
• Genome accession: NZ_CP169520
• Coordinates: 2543397..2544011 (-)
• Length: 204 a.a.
• NCBI ID: WP_043867297.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain CX-H3
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2538397..2549011

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACEX67_RS12200 (ACEX67_12200)	rpsT	2538697..2538963 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACEX67_RS12205 (ACEX67_12205)	holA	2538980..2540021 (-)	1042	Protein_2356	DNA polymerase III subunit delta	-
ACEX67_RS12210 (ACEX67_12210)	-	2540061..2540215 (-)	155	Protein_2357	hypothetical protein	-
ACEX67_RS12215 (ACEX67_12215)	-	2540256..2540390 (+)	135	WP_003152870.1	YqzM family protein	-
ACEX67_RS12220 (ACEX67_12220)	comEC	2540409..2542760 (-)	2352	WP_234949402.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACEX67_RS12225 (ACEX67_12225)	-	2542761..2543330 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACEX67_RS12230 (ACEX67_12230)	comEA	2543397..2544011 (-)	615	WP_043867297.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACEX67_RS12235 (ACEX67_12235)	comER	2544070..2544891 (+)	822	WP_014305449.1	late competence protein ComER	-
ACEX67_RS12240 (ACEX67_12240)	-	2544960..2545697 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
ACEX67_RS12245 (ACEX67_12245)	rsfS	2545694..2546050 (-)	357	WP_003152864.1	ribosome silencing factor	-
ACEX67_RS12250 (ACEX67_12250)	yqeK	2546047..2546628 (-)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACEX67_RS12255 (ACEX67_12255)	-	2546618..2547187 (-)	570	WP_160249251.1	nicotinate-nucleotide adenylyltransferase	-
ACEX67_RS12260 (ACEX67_12260)	yhbY	2547198..2547488 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ACEX67_RS12265 (ACEX67_12265)	aroE	2547482..2548324 (-)	843	WP_160249250.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22178.37 Da
• Isoelectric Point: 6.9685

>NTDB_id=1051665 ACEX67_RS12230 WP_043867297.1 2543397..2544011(-) (comEA) [Bacillus velezensis strain CX-H3]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEGNHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1051665 ACEX67_RS12230 WP_043867297.1 2543397..2544011(-) (comEA) [Bacillus velezensis strain CX-H3]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGGGAATCATCCGAAAACAG
CAGTTGGTGAAAAGGAAAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCAATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363