Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   O1Q80_RS09370 Genome accession   NZ_CP169368
Coordinates   1984224..1985672 (-) Length   482 a.a.
NCBI ID   WP_386694672.1    Uniprot ID   -
Organism   Lonepinella sp. MS14435     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1979224..1990672
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O1Q80_RS09340 (O1Q80_01862) - 1979411..1980778 (-) 1368 WP_386694667.1 alanine/glycine:cation symporter family protein -
  O1Q80_RS09345 (O1Q80_01863) - 1981191..1981457 (-) 267 WP_386690985.1 GNAT family N-acetyltransferase -
  O1Q80_RS09350 (O1Q80_01864) yacG 1981460..1981660 (-) 201 WP_132301902.1 DNA gyrase inhibitor YacG -
  O1Q80_RS09355 (O1Q80_01865) coaE 1981644..1982273 (-) 630 WP_386694669.1 dephospho-CoA kinase -
  O1Q80_RS09360 (O1Q80_01866) - 1982299..1982961 (-) 663 WP_386694671.1 prepilin peptidase -
  O1Q80_RS09365 (O1Q80_01867) pilC 1982994..1984214 (-) 1221 WP_386687254.1 type II secretion system F family protein Machinery gene
  O1Q80_RS09370 (O1Q80_01868) pilB 1984224..1985672 (-) 1449 WP_386694672.1 GspE/PulE family protein Machinery gene
  O1Q80_RS09375 (O1Q80_01869) pilA 1985672..1986166 (-) 495 WP_386694674.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  O1Q80_RS09380 (O1Q80_01870) ampD 1986311..1986856 (+) 546 WP_386694675.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  O1Q80_RS09385 (O1Q80_01871) - 1986860..1987510 (+) 651 WP_386687262.1 HAD family hydrolase -
  O1Q80_RS09390 (O1Q80_01872) - 1987762..1988031 (+) 270 WP_153984892.1 PTS sugar transporter subunit IIB -
  O1Q80_RS09395 (O1Q80_01873) - 1988041..1989396 (+) 1356 WP_153984893.1 PTS ascorbate transporter subunit IIC -
  O1Q80_RS09400 (O1Q80_01874) - 1989397..1990215 (+) 819 WP_386694677.1 transketolase -

Sequence

Protein


Download         Length: 482 a.a.        Molecular weight: 54293.25 Da        Isoelectric Point: 7.0261

>NTDB_id=1051032 O1Q80_RS09370 WP_386694672.1 1984224..1985672(-) (pilB) [Lonepinella sp. MS14435]
MQKSLSSPHIVSSNVVSPNVVSPNVVSHDGKIFQISPQLWQQNQQQQNILIRYLACPLAENERCLWLGLDSLNNLSACET
FSFLTGKMIEPVLLETQVLKDLLQALAPQSLMVQESPVFYHVQQQDLNEEKHSTQQSDEPVIQLLNRIFESALQQRASDI
HLEPQSQHLQVRFRIDGVLQHQPPVALALSSRVISRLKLLAKLDISETRLPQDGRFHFKTTFSDMLDFRLSTLPTPFGEK
AVLRVQQNKPVQFAFADLGMNDQQQERFKYALNQPQGLILVTGPTGSGKSITLYTALSYLNRPEKHIMTAEDPIEIPLSG
IIQSQVNPQIGLDFSRLLRTFLRQDPDIIMLGEIRDEESAAMALRASQTGHLVLSTLHTNDAPSAIPRLLQLGIKQHEID
NSLLLVIAQRLIRKRCQKCGGKSENSCDCHQGYKGRIGIYQFLQPDPKQGYQLDVDHLQQAGLEKVKQGITDLVEIQRVL
GH

Nucleotide


Download         Length: 1449 bp        

>NTDB_id=1051032 O1Q80_RS09370 WP_386694672.1 1984224..1985672(-) (pilB) [Lonepinella sp. MS14435]
ATGCAAAAGAGTCTATCTAGTCCACATATTGTTAGCTCAAATGTTGTTAGCCCGAATGTTGTTAGCCCGAATGTGGTGAG
CCATGACGGAAAAATATTCCAAATTTCACCGCAGTTATGGCAACAAAATCAGCAACAACAGAACATTCTGATCCGTTATT
TAGCTTGTCCATTAGCGGAAAATGAGCGTTGTTTATGGCTCGGGCTAGATAGCCTCAACAATTTATCCGCTTGTGAAACC
TTTTCTTTCCTAACAGGAAAAATGATCGAACCTGTGTTGTTGGAAACACAGGTATTGAAAGATTTATTGCAAGCCTTAGC
TCCACAATCTTTAATGGTGCAAGAAAGCCCTGTTTTTTACCATGTTCAACAGCAAGATTTAAACGAAGAAAAGCATTCTA
CTCAACAATCAGATGAACCCGTGATTCAGTTGCTTAATCGGATTTTTGAAAGTGCGTTGCAGCAACGAGCATCTGATATT
CACCTTGAGCCACAAAGCCAACATTTGCAAGTGCGTTTTCGTATTGATGGTGTATTACAACACCAACCGCCAGTTGCATT
AGCATTATCTAGTAGGGTGATTTCACGTTTAAAATTATTAGCCAAACTAGATATTAGCGAAACTCGCTTACCGCAAGACG
GACGTTTTCATTTTAAAACCACCTTTTCTGATATGTTAGATTTTCGTCTTTCCACCTTACCAACTCCATTTGGGGAAAAA
GCGGTGTTGCGGGTGCAACAAAATAAGCCAGTACAATTTGCATTTGCCGATCTGGGTATGAATGATCAGCAGCAGGAACG
TTTTAAATACGCCCTTAATCAACCGCAAGGCTTGATTTTAGTGACAGGACCTACGGGAAGTGGGAAAAGTATCACACTTT
ACACCGCACTTTCTTATTTAAATCGTCCTGAAAAACATATTATGACCGCGGAAGATCCTATTGAAATTCCATTGTCTGGC
ATTATTCAAAGCCAAGTCAATCCACAAATTGGTTTAGATTTCAGTCGGTTATTGCGTACTTTCTTACGCCAAGATCCCGA
TATTATTATGCTCGGTGAAATTCGTGATGAAGAAAGTGCTGCAATGGCATTACGAGCTTCTCAAACGGGGCATCTTGTGC
TTTCCACGTTGCATACCAATGATGCACCGTCCGCTATTCCACGCTTGTTACAGTTAGGTATAAAGCAACACGAAATTGAC
AACAGCTTATTGTTAGTGATTGCACAACGTCTGATTCGGAAACGTTGCCAAAAGTGCGGTGGAAAAAGCGAAAATTCTTG
TGATTGTCATCAAGGCTATAAAGGTCGAATCGGGATTTATCAATTTTTACAACCTGATCCAAAGCAAGGTTATCAGTTGG
ATGTTGATCATTTACAACAAGCAGGGCTAGAGAAAGTGAAGCAAGGAATAACGGATTTAGTGGAAATTCAACGAGTATTA
GGACATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(147-414)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

70.022

94.813

0.664
  pilB Haemophilus influenzae Rd KW20

69.147

94.813

0.656
  pilB Glaesserella parasuis strain SC1401

59.041

95.228

0.562
  pilB Vibrio parahaemolyticus RIMD 2210633

39.961

100

0.429
  pilB Vibrio campbellii strain DS40M4

41.529

100

0.417
  pilB Acinetobacter baylyi ADP1

36.862

100

0.405
  pilB Legionella pneumophila strain ERS1305867

39.388

100

0.4
  pilB Vibrio cholerae strain A1552

39.05

100

0.392
  pilB Acinetobacter baumannii D1279779

38.193

100

0.386
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.667

99.585

0.365

Multiple sequence alignment