Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   O1Q80_RS09375 Genome accession   NZ_CP169368
Coordinates   1985672..1986166 (-) Length   164 a.a.
NCBI ID   WP_386694674.1    Uniprot ID   -
Organism   Lonepinella sp. MS14435     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1980672..1991166
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O1Q80_RS09345 (O1Q80_01863) - 1981191..1981457 (-) 267 WP_386690985.1 GNAT family N-acetyltransferase -
  O1Q80_RS09350 (O1Q80_01864) yacG 1981460..1981660 (-) 201 WP_132301902.1 DNA gyrase inhibitor YacG -
  O1Q80_RS09355 (O1Q80_01865) coaE 1981644..1982273 (-) 630 WP_386694669.1 dephospho-CoA kinase -
  O1Q80_RS09360 (O1Q80_01866) - 1982299..1982961 (-) 663 WP_386694671.1 prepilin peptidase -
  O1Q80_RS09365 (O1Q80_01867) pilC 1982994..1984214 (-) 1221 WP_386687254.1 type II secretion system F family protein Machinery gene
  O1Q80_RS09370 (O1Q80_01868) pilB 1984224..1985672 (-) 1449 WP_386694672.1 GspE/PulE family protein Machinery gene
  O1Q80_RS09375 (O1Q80_01869) pilA 1985672..1986166 (-) 495 WP_386694674.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  O1Q80_RS09380 (O1Q80_01870) ampD 1986311..1986856 (+) 546 WP_386694675.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  O1Q80_RS09385 (O1Q80_01871) - 1986860..1987510 (+) 651 WP_386687262.1 HAD family hydrolase -
  O1Q80_RS09390 (O1Q80_01872) - 1987762..1988031 (+) 270 WP_153984892.1 PTS sugar transporter subunit IIB -
  O1Q80_RS09395 (O1Q80_01873) - 1988041..1989396 (+) 1356 WP_153984893.1 PTS ascorbate transporter subunit IIC -
  O1Q80_RS09400 (O1Q80_01874) - 1989397..1990215 (+) 819 WP_386694677.1 transketolase -
  O1Q80_RS09405 (O1Q80_01875) - 1990212..1991156 (+) 945 WP_386694678.1 transketolase family protein -

Sequence

Protein


Download         Length: 164 a.a.        Molecular weight: 17219.52 Da        Isoelectric Point: 8.4488

>NTDB_id=1051033 O1Q80_RS09375 WP_386694674.1 1985672..1986166(-) (pilA) [Lonepinella sp. MS14435]
MYFNRTLIQTSYNLQFKKGFTLIELMIVIAIVAILATIAIPSYQNYTKKAAISELLQAAAPYRSEVELCVYNLDKPDNCS
AGQNGIQANITDGSGKYVKSVAVSAGKITVTGKGSVADVSYTLTASGSASTGVTWAANCGTKSELFPAGFCSTNSTSSTS
TGQQ

Nucleotide


Download         Length: 495 bp        

>NTDB_id=1051033 O1Q80_RS09375 WP_386694674.1 1985672..1986166(-) (pilA) [Lonepinella sp. MS14435]
ATGTATTTTAACCGTACTTTGATTCAAACTTCGTACAATCTACAGTTCAAAAAAGGCTTTACCCTGATTGAATTGATGAT
CGTGATTGCCATTGTGGCAATTTTAGCCACCATTGCAATTCCATCTTACCAAAATTATACCAAAAAAGCCGCCATTTCTG
AACTGTTGCAAGCAGCAGCACCTTATCGTTCCGAAGTAGAGCTTTGTGTTTATAACTTAGATAAACCAGATAATTGCTCC
GCAGGTCAAAATGGGATCCAAGCAAATATCACAGATGGTTCAGGCAAATATGTGAAATCTGTTGCCGTCAGTGCTGGCAA
GATTACCGTGACGGGGAAAGGTAGTGTTGCTGATGTGAGTTACACTTTAACTGCCAGTGGTTCAGCTAGTACAGGGGTAA
CTTGGGCGGCAAACTGCGGTACAAAATCGGAATTATTTCCAGCTGGTTTCTGTAGCACAAATAGTACAAGTAGTACAAGC
ACTGGACAACAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(49-149)

Prokaryotic N-terminal methylation motif (N_methyl)

(17-39)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

66.423

83.537

0.555
  pilA Haemophilus influenzae Rd KW20

62.774

83.537

0.524
  pilA Glaesserella parasuis strain SC1401

54.545

87.195

0.476

Multiple sequence alignment