Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACEWE4_RS04560 Genome accession   NZ_CP169311
Coordinates   890084..891484 (+) Length   466 a.a.
NCBI ID   WP_040080755.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain Mh184.20240815.PV.SD     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 885084..896484
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEWE4_RS04530 (ACEWE4_000907) - 885158..885922 (-) 765 WP_006248395.1 TSUP family transporter -
  ACEWE4_RS04535 (ACEWE4_000908) proB 885922..887013 (-) 1092 WP_006251346.1 glutamate 5-kinase -
  ACEWE4_RS04540 (ACEWE4_000909) folA 887125..887619 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  ACEWE4_RS04545 (ACEWE4_000910) - 887739..887969 (+) 231 WP_006248398.1 YgjV family protein -
  ACEWE4_RS04550 (ACEWE4_000911) radA 888024..889403 (-) 1380 WP_040080753.1 DNA repair protein RadA Machinery gene
  ACEWE4_RS04555 (ACEWE4_000912) pilA 889584..890063 (+) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  ACEWE4_RS04560 (ACEWE4_000913) pilB 890084..891484 (+) 1401 WP_040080755.1 GspE/PulE family protein Machinery gene
  ACEWE4_RS04565 (ACEWE4_000914) pilC 891468..892664 (+) 1197 WP_006248403.1 type II secretion system F family protein Machinery gene
  ACEWE4_RS04570 (ACEWE4_000915) - 892667..893323 (+) 657 WP_006248404.1 prepilin peptidase -
  ACEWE4_RS04575 (ACEWE4_000916) coaE 893359..893994 (+) 636 WP_020831305.1 dephospho-CoA kinase -
  ACEWE4_RS04580 (ACEWE4_000917) yacG 893976..894161 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  ACEWE4_RS04585 (ACEWE4_000918) holA 894162..895187 (-) 1026 WP_006253426.1 DNA polymerase III subunit delta -
  ACEWE4_RS04590 (ACEWE4_000919) lptE 895230..895718 (-) 489 WP_006251337.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 466 a.a.        Molecular weight: 52950.61 Da        Isoelectric Point: 5.9587

>NTDB_id=1050356 ACEWE4_RS04560 WP_040080755.1 890084..891484(+) (pilB) [Mannheimia haemolytica strain Mh184.20240815.PV.SD]
MEYSVTDLNSQRFIEISAEQWQKNCNEKQILLRYLAIPLQETEEQLWLGIDDMQNLNACEIFAFIYHKQIEPVLISSDEL
KFLLNALSPEQSSRQSEIYEENTAYSLAHSEQVDRNDPIIQILENLFKFCLQHNASDIHFEPQKEKLLIRLRIDGVLHIY
KTLPQSLSSRLISRIKLLAKLDISETRLPQDGQFHFKTILAETLDFRVSTLPTHFGEKVVLRLQKNKPTQVDFLELGFNP
TQKANLRNALSQPQGLILVTGPTGSGKSITLYSALSHLNSSEKHILTAEDPIEIEIEGIIQTQVNRGINLDFSQLLRTFL
RQDPDIIMLGEIRDEESAEMALRAAQTGHLVLSTLHTNDAPSAVERLLQLGIKEYELKNSLLLVIAQRLLRRVCVKCGGA
GCEHCYQGYKGRIGVYQLLNRSAKNFEKNTACLDFKTLADSAKEKLTEGKTNQAEILRVLGNDENL

Nucleotide


Download         Length: 1401 bp        

>NTDB_id=1050356 ACEWE4_RS04560 WP_040080755.1 890084..891484(+) (pilB) [Mannheimia haemolytica strain Mh184.20240815.PV.SD]
ATGGAGTATTCGGTAACGGATTTAAACAGCCAAAGGTTCATCGAGATTTCAGCAGAGCAGTGGCAGAAAAATTGCAATGA
AAAGCAGATTTTATTGCGATATTTAGCCATTCCGCTGCAAGAAACAGAAGAACAGCTTTGGCTAGGGATTGATGATATGC
AAAATCTCAATGCCTGTGAGATTTTTGCGTTTATCTACCACAAACAGATTGAGCCTGTTTTGATTTCCTCCGATGAATTG
AAGTTTTTACTCAATGCCCTTTCGCCTGAGCAGAGTAGCCGGCAGAGTGAGATTTATGAGGAGAATACGGCGTATTCATT
GGCTCATTCGGAACAAGTGGATAGAAACGATCCGATTATCCAAATTTTAGAGAATTTATTTAAGTTCTGTTTGCAACACA
ACGCCTCAGATATTCATTTTGAACCACAAAAAGAGAAATTGCTTATTCGGCTGCGAATTGATGGCGTGCTACATATTTAT
AAAACGCTTCCGCAAAGTCTGTCTAGCCGATTAATTTCTCGCATTAAATTATTGGCAAAATTAGATATTAGCGAAACCCG
CTTGCCGCAAGATGGGCAGTTTCATTTTAAAACAATTTTAGCGGAAACCTTAGATTTCCGCGTTTCAACTTTACCTACCC
ATTTCGGTGAAAAAGTTGTTCTTCGGCTTCAAAAGAATAAGCCTACGCAGGTGGATTTTTTAGAATTGGGGTTTAACCCC
ACTCAGAAAGCCAATTTGCGGAATGCTCTGAGCCAACCTCAAGGGTTGATTTTAGTCACAGGCCCGACCGGGAGCGGTAA
AAGCATCACGCTTTACAGTGCATTAAGCCATTTAAACAGCAGTGAAAAGCATATTTTAACTGCCGAAGACCCGATTGAAA
TTGAGATTGAGGGAATTATCCAAACCCAAGTGAATCGAGGGATTAATTTAGATTTCAGTCAACTGTTAAGAACTTTTCTA
CGCCAAGATCCGGACATTATTATGCTTGGCGAAATTCGTGATGAAGAGAGTGCAGAAATGGCACTACGGGCGGCACAAAC
CGGGCATTTGGTGCTTTCAACTTTGCATACCAATGATGCCCCCTCAGCGGTAGAACGGTTGCTACAGCTCGGCATAAAAG
AGTATGAACTTAAAAATTCATTGCTACTCGTGATTGCTCAACGCCTGTTACGCCGAGTGTGTGTGAAATGCGGTGGGGCA
GGCTGTGAGCATTGTTATCAAGGGTATAAAGGGCGAATTGGGGTATATCAACTCTTAAACCGATCTGCAAAAAATTTTGA
AAAAAACACCGCTTGTTTGGATTTTAAAACCCTTGCCGACAGTGCAAAAGAAAAATTGACGGAAGGAAAAACCAATCAGG
CGGAAATTTTAAGGGTGCTAGGCAATGACGAAAATCTATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-92)

Type II/IV secretion system protein (T2SSE)

(131-392)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

72.234

98.927

0.715
  pilB Haemophilus influenzae Rd KW20

57.235

99.356

0.569
  pilB Haemophilus influenzae 86-028NP

56.466

99.571

0.562
  pilB Vibrio cholerae strain A1552

40.871

100

0.423
  pilB Vibrio campbellii strain DS40M4

40.541

100

0.418
  pilB Vibrio parahaemolyticus RIMD 2210633

38.833

100

0.414
  pilB Legionella pneumophila strain ERS1305867

39.549

100

0.414
  pilB Acinetobacter baumannii D1279779

36.605

100

0.384
  pilB Acinetobacter baylyi ADP1

36.68

100

0.384

Multiple sequence alignment