Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACEWE4_RS04550 Genome accession   NZ_CP169311
Coordinates   888024..889403 (-) Length   459 a.a.
NCBI ID   WP_040080753.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain Mh184.20240815.PV.SD     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 883024..894403
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEWE4_RS04520 (ACEWE4_000905) - 883076..884326 (-) 1251 WP_040080752.1 multifunctional CCA addition/repair protein -
  ACEWE4_RS04525 (ACEWE4_000906) nth 884523..885155 (-) 633 WP_006248394.1 endonuclease III -
  ACEWE4_RS04530 (ACEWE4_000907) - 885158..885922 (-) 765 WP_006248395.1 TSUP family transporter -
  ACEWE4_RS04535 (ACEWE4_000908) proB 885922..887013 (-) 1092 WP_006251346.1 glutamate 5-kinase -
  ACEWE4_RS04540 (ACEWE4_000909) folA 887125..887619 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  ACEWE4_RS04545 (ACEWE4_000910) - 887739..887969 (+) 231 WP_006248398.1 YgjV family protein -
  ACEWE4_RS04550 (ACEWE4_000911) radA 888024..889403 (-) 1380 WP_040080753.1 DNA repair protein RadA Machinery gene
  ACEWE4_RS04555 (ACEWE4_000912) pilA 889584..890063 (+) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  ACEWE4_RS04560 (ACEWE4_000913) pilB 890084..891484 (+) 1401 WP_040080755.1 GspE/PulE family protein Machinery gene
  ACEWE4_RS04565 (ACEWE4_000914) pilC 891468..892664 (+) 1197 WP_006248403.1 type II secretion system F family protein Machinery gene
  ACEWE4_RS04570 (ACEWE4_000915) - 892667..893323 (+) 657 WP_006248404.1 prepilin peptidase -
  ACEWE4_RS04575 (ACEWE4_000916) coaE 893359..893994 (+) 636 WP_020831305.1 dephospho-CoA kinase -
  ACEWE4_RS04580 (ACEWE4_000917) yacG 893976..894161 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49759.39 Da        Isoelectric Point: 8.1850

>NTDB_id=1050354 ACEWE4_RS04550 WP_040080753.1 888024..889403(-) (radA) [Mannheimia haemolytica strain Mh184.20240815.PV.SD]
MAKAPKTAYVCNDCGAEYSRWMGQCKECKAWNTISEVRLISSKEAAKSDRFSGYAGETSGKVQTLSDISLQEVPRFSSGF
NELDRVLGGGVVPGSAILIGGHPGAGKSTLLLQVMCGLAQHLPTLYVTGEESLQQVAMRANRLGLPTANLKMLSETSVEH
ICNIADQEKPKIMVIDSIQVMHLSDIQSSPGSVSQVRECASFLTRYAKTRQVAIIMVGHVTKDGTLAGPKVLEHAIDASL
LLEGESDSRFRTLRSQKNRFGAVNELGVFAMTEQGLKEVKNPSAIFLSRSEEQTPGSSVMVLWEGTRPLLVEIQALVDHS
MLANPRRVAVGLDHNRLSLLLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALILALISSFRNRPLPQDLVIFGEVG
LAGEIRPVTSGQERISEAAKHGFKRAIIPHGNVPKKAIKDMQVFTVKKLSDALDILYNL

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=1050354 ACEWE4_RS04550 WP_040080753.1 888024..889403(-) (radA) [Mannheimia haemolytica strain Mh184.20240815.PV.SD]
ATGGCAAAAGCACCTAAAACCGCTTATGTTTGTAACGATTGCGGGGCTGAATATTCCCGTTGGATGGGGCAATGTAAGGA
ATGTAAGGCTTGGAATACCATCAGTGAAGTGCGGTTGATTTCCAGCAAAGAAGCGGCGAAATCTGACCGCTTTAGCGGCT
ATGCCGGTGAAACGTCAGGCAAAGTACAAACGCTGTCTGACATCAGCTTGCAGGAAGTGCCTCGCTTTAGCAGTGGCTTT
AACGAGCTGGATCGTGTACTCGGTGGCGGCGTAGTGCCGGGGTCAGCAATTCTGATCGGCGGTCACCCGGGGGCGGGGAA
AAGTACGTTATTACTGCAAGTAATGTGCGGTTTAGCCCAACATCTGCCAACGCTGTATGTCACCGGTGAAGAGTCGTTAC
AACAAGTTGCAATGCGGGCAAATCGCTTAGGCTTACCAACGGCAAATCTAAAAATGTTGTCTGAAACCTCTGTCGAGCAT
ATTTGCAATATTGCAGATCAAGAAAAGCCCAAAATTATGGTGATTGACTCGATTCAGGTGATGCACTTGTCGGATATTCA
GTCCTCACCCGGCAGTGTTTCACAAGTGCGTGAATGTGCATCTTTCCTTACCCGCTACGCCAAAACCCGCCAAGTGGCGA
TTATTATGGTTGGGCACGTCACTAAAGATGGCACGCTTGCCGGCCCGAAAGTGTTGGAACACGCCATCGATGCTTCCTTG
CTGTTAGAAGGCGAATCCGATTCCCGTTTCCGTACTTTGCGTAGCCAGAAAAATCGTTTTGGTGCCGTCAATGAATTGGG
CGTATTTGCGATGACCGAACAAGGGCTAAAAGAGGTTAAAAACCCATCTGCGATTTTTTTAAGCCGTAGCGAAGAGCAAA
CGCCGGGCAGTTCGGTGATGGTGTTATGGGAAGGAACACGCCCATTGTTGGTTGAAATTCAAGCCTTAGTCGATCACTCA
ATGCTGGCAAACCCTCGCCGTGTGGCGGTTGGCTTAGATCATAACCGTTTATCGCTATTACTTGCGGTGCTACACCGACA
CGGTGGCTTGCAGATGTCGGATCAAGATGTGTTTGTAAATGTGGTCGGTGGGGTGAAAGTCACGGAAACCAGTGCCGATT
TAGCCTTAATTTTGGCGTTAATTTCCAGCTTCCGTAACCGTCCATTGCCGCAAGATTTGGTGATTTTTGGGGAAGTCGGC
TTAGCAGGGGAAATCCGCCCGGTTACCAGTGGGCAAGAGCGGATCAGCGAAGCCGCAAAACACGGCTTTAAACGGGCGAT
AATTCCTCACGGCAATGTCCCGAAAAAGGCAATTAAAGATATGCAGGTGTTCACCGTGAAAAAACTCAGTGATGCGTTAG
ATATTCTGTATAATCTCTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(347-432)

AAA domain (AAA_25)

(84-224)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.465

99.346

0.481
  radA Streptococcus mitis NCTC 12261

44.273

98.911

0.438
  radA Streptococcus mitis SK321

44.053

98.911

0.436
  radA Streptococcus pneumoniae TIGR4

43.573

100

0.436
  radA Streptococcus pneumoniae D39

43.573

100

0.436
  radA Streptococcus pneumoniae Rx1

43.573

100

0.436
  radA Streptococcus pneumoniae R6

43.573

100

0.436

Multiple sequence alignment