Detailed information NTG1049748

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACETWW_RS12200
• Genome accession: NZ_CP169072
• Coordinates: 2534532..2535146 (-)
• Length: 204 a.a.
• NCBI ID: WP_043021637.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain T971
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2529532..2540146

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACETWW_RS12170 (ACETWW_12160)	rpsT	2529835..2530101 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACETWW_RS12175 (ACETWW_12165)	holA	2530118..2531159 (-)	1042	Protein_2348	DNA polymerase III subunit delta	-
ACETWW_RS12180 (ACETWW_12170)	-	2531199..2531350 (-)	152	Protein_2349	hypothetical protein	-
ACETWW_RS12185 (ACETWW_12175)	-	2531391..2531525 (+)	135	WP_003152870.1	YqzM family protein	-
ACETWW_RS12190 (ACETWW_12180)	comEC	2531544..2533895 (-)	2352	WP_374004364.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACETWW_RS12195 (ACETWW_12185)	-	2533896..2534465 (-)	570	WP_094031848.1	ComE operon protein 2	-
ACETWW_RS12200 (ACETWW_12190)	comEA	2534532..2535146 (-)	615	WP_043021637.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACETWW_RS12205 (ACETWW_12195)	comER	2535205..2536026 (+)	822	WP_076424996.1	late competence protein ComER	-
ACETWW_RS12210 (ACETWW_12200)	-	2536095..2536832 (-)	738	WP_076424998.1	class I SAM-dependent DNA methyltransferase	-
ACETWW_RS12215 (ACETWW_12205)	rsfS	2536829..2537185 (-)	357	WP_007408260.1	ribosome silencing factor	-
ACETWW_RS12220 (ACETWW_12210)	yqeK	2537203..2537763 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACETWW_RS12225 (ACETWW_12215)	-	2537753..2538322 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
ACETWW_RS12230 (ACETWW_12220)	yhbY	2538333..2538623 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
ACETWW_RS12235 (ACETWW_12225)	aroE	2538617..2539459 (-)	843	WP_025285019.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22165.31 Da
• Isoelectric Point: 6.4888

>NTDB_id=1049748 ACETWW_RS12200 WP_043021637.1 2534532..2535146(-) (comEA) [Bacillus velezensis strain T971]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1049748 ACETWW_RS12200 WP_043021637.1 2534532..2535146(-) (comEA) [Bacillus velezensis strain T971]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363