Detailed information NTG1048967

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACEQ85_RS03430
• Genome accession: NZ_CP168966
• Coordinates: 740471..741589 (+)
• Length: 372 a.a.
• NCBI ID: WP_032037409.1
• Uniprot ID: A0A013T0Z7
• Organism: Acinetobacter pittii strain LS-AP
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 735471..746589

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACEQ85_RS03420 (ACEQ85_03420)	yggS	738595..739287 (-)	693	WP_202732893.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACEQ85_RS03425 (ACEQ85_03425)	pilT	739414..740451 (+)	1038	WP_002121749.1	type IV pilus twitching motility protein PilT	Machinery gene
ACEQ85_RS03430 (ACEQ85_03430)	pilU	740471..741589 (+)	1119	WP_032037409.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACEQ85_RS03435 (ACEQ85_03435)	fur	741659..742096 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
ACEQ85_RS03440 (ACEQ85_03440)	omlA	742208..742606 (+)	399	WP_373883370.1	outer membrane protein assembly factor BamE	-
ACEQ85_RS03445 (ACEQ85_03445)	-	742671..742985 (-)	315	WP_086264827.1	RnfH family protein	-
ACEQ85_RS03450 (ACEQ85_03450)	-	742985..744052 (-)	1068	WP_373883371.1	hypothetical protein	-
ACEQ85_RS03455 (ACEQ85_03455)	-	744206..744661 (-)	456	WP_002121698.1	bacteriohemerythrin	-
ACEQ85_RS03460 (ACEQ85_03460)	-	744913..745761 (-)	849	WP_017398883.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41947.15 Da
• Isoelectric Point: 6.6403

>NTDB_id=1048967 ACEQ85_RS03430 WP_032037409.1 740471..741589(+) (pilU) [Acinetobacter pittii strain LS-AP]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEESPDQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=1048967 ACEQ85_RS03430 WP_032037409.1 740471..741589(+) (pilU) [Acinetobacter pittii strain LS-AP]
ATGGATTTTAATGACCTACTCAACCTAATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATTGGACAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCTGAGACTCGTGAATGTAACTTTGCCATTATGAACCGTGATAAAACTGCC
CGCTTTCGTGTGAGTGCTTTTCAGCAACGCGACATGCCAGGCATGGTACTACGTCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTGCAATTACCACCTGTGCTTAAAGATTTATCGATGACCAAACGCGGCATCATTATTTTTGTAGGGGCAACAG
GTACAGGTAAATCGACTTCTTTAGCTTCAATGATTAGCTATCGGAACCATAACTCTAAAGGTCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCATGAACATGCTGGCTGCATCATTACTCAACGTGAAGTCGGAATTGATACAGACTCATTTGA
AATTGCCTTAAAGAATACTTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATTCGCTCTCGTGAAGTTATGGACT
ACGCGATTGGCTTCGCCGAAACAGGTCACCTTGTATTAGCCACAATGCATGCGAACAACGCCAACCAAGCACTTGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGATTTATCACTTAACTTAAAGGCCATGATTGCACA
GCAACTTATTCCAACGCCTGATGGTAATTCGCGCCGTGCAGCGATTGAGATTCTCATTAACTCACCACTAATTTCAGACT
ATATTCGCAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTTTATAAGTCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTGCG
TTTAACAATTAAGCTTGCCGAAGAAAGTCCTGATCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACCGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (33-270)

3D structure

• AlphaFold DB: A0A013T0Z7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.397	100	0.766
pilU	Pseudomonas stutzeri DSM 10701	68.464	99.731	0.683
pilU	Vibrio cholerae strain A1552	53.784	99.462	0.535
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.934	97.849	0.401
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363