Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACEQ38_RS12725 Genome accession   NZ_CP168747
Coordinates   2755614..2756120 (+) Length   168 a.a.
NCBI ID   WP_075465281.1    Uniprot ID   A0A1U9VGI2
Organism   Ralstonia syzygii subsp. celebesensis strain NCPPB 3727     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2750614..2761120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEQ38_RS12700 (ACEQ38_12700) - 2750957..2751583 (+) 627 WP_013213432.1 DUF2889 domain-containing protein -
  ACEQ38_RS12705 (ACEQ38_12705) sucC 2751651..2752817 (+) 1167 WP_013213431.1 ADP-forming succinate--CoA ligase subunit beta -
  ACEQ38_RS12710 (ACEQ38_12710) sucD 2752902..2753783 (+) 882 WP_013213430.1 succinate--CoA ligase subunit alpha -
  ACEQ38_RS12715 (ACEQ38_12715) - 2753938..2754636 (+) 699 WP_078222164.1 TerC family protein -
  ACEQ38_RS12720 (ACEQ38_12720) pilA 2754830..2755351 (+) 522 WP_078222165.1 pilin Machinery gene
  ACEQ38_RS12725 (ACEQ38_12725) pilA 2755614..2756120 (+) 507 WP_075465281.1 pilin Machinery gene
  ACEQ38_RS12730 (ACEQ38_12730) - 2756197..2757933 (+) 1737 WP_078222166.1 PglL family O-oligosaccharyltransferase -
  ACEQ38_RS12735 (ACEQ38_12735) moaC 2758007..2758483 (-) 477 WP_078222167.1 cyclic pyranopterin monophosphate synthase MoaC -
  ACEQ38_RS12740 (ACEQ38_12740) - 2758578..2760266 (+) 1689 WP_078222168.1 M48 family metallopeptidase -
  ACEQ38_RS12745 (ACEQ38_12745) - 2760285..2760872 (-) 588 WP_078222169.1 DUF2946 family protein -

Sequence

Protein


Download         Length: 168 a.a.        Molecular weight: 16899.53 Da        Isoelectric Point: 9.9749

>NTDB_id=1045474 ACEQ38_RS12725 WP_075465281.1 2755614..2756120(+) (pilA) [Ralstonia syzygii subsp. celebesensis strain NCPPB 3727]
MKSMRHLNKRVQKGFTLIELMIVVAIVGILAAIAIPAYQDYTIRARVTEGLSLAAQAKSLVAENAANAQTDLSVGSSTFA
ATKNVSALAINATAAPAGQITITYTTAAGNGTLALVPTSGGSALVVSSAPGAAIMWTCYSAGKATAASSVAASPSATLLP
KYAPAECR

Nucleotide


Download         Length: 507 bp        

>NTDB_id=1045474 ACEQ38_RS12725 WP_075465281.1 2755614..2756120(+) (pilA) [Ralstonia syzygii subsp. celebesensis strain NCPPB 3727]
ATGAAGTCGATGCGTCATCTCAACAAGCGTGTCCAGAAGGGCTTCACGCTGATCGAACTGATGATCGTGGTCGCGATCGT
CGGTATTCTGGCTGCGATTGCCATTCCGGCCTATCAGGACTACACCATCCGTGCACGCGTGACCGAAGGTCTGTCGCTGG
CTGCTCAAGCCAAGTCGCTGGTGGCTGAGAACGCTGCAAACGCTCAGACCGACCTGTCGGTGGGTTCGTCGACGTTTGCG
GCTACCAAGAACGTGTCCGCTCTTGCTATTAACGCGACTGCGGCGCCCGCCGGCCAAATCACGATTACGTACACCACTGC
AGCGGGTAACGGTACGCTTGCCCTCGTCCCGACCTCGGGAGGTAGTGCTTTGGTCGTGAGTTCCGCTCCGGGCGCAGCTA
TCATGTGGACGTGCTACTCCGCTGGTAAGGCTACGGCTGCCAGCAGCGTTGCTGCAAGTCCTTCGGCGACCCTGCTGCCG
AAGTATGCTCCGGCAGAATGCCGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(44-168)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1U9VGI2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

78.571

100

0.786
  pilA2 Legionella pneumophila str. Paris

52.174

95.833

0.5
  pilA2 Legionella pneumophila strain ERS1305867

52.174

95.833

0.5
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.879

100

0.446
  comP Acinetobacter baylyi ADP1

43.275

100

0.44

Multiple sequence alignment