Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACEQ38_RS12720 Genome accession   NZ_CP168747
Coordinates   2754830..2755351 (+) Length   173 a.a.
NCBI ID   WP_078222165.1    Uniprot ID   -
Organism   Ralstonia syzygii subsp. celebesensis strain NCPPB 3727     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2749830..2760351
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEQ38_RS12695 (ACEQ38_12695) recX 2750205..2750684 (+) 480 WP_013213433.1 recombination regulator RecX -
  ACEQ38_RS12700 (ACEQ38_12700) - 2750957..2751583 (+) 627 WP_013213432.1 DUF2889 domain-containing protein -
  ACEQ38_RS12705 (ACEQ38_12705) sucC 2751651..2752817 (+) 1167 WP_013213431.1 ADP-forming succinate--CoA ligase subunit beta -
  ACEQ38_RS12710 (ACEQ38_12710) sucD 2752902..2753783 (+) 882 WP_013213430.1 succinate--CoA ligase subunit alpha -
  ACEQ38_RS12715 (ACEQ38_12715) - 2753938..2754636 (+) 699 WP_078222164.1 TerC family protein -
  ACEQ38_RS12720 (ACEQ38_12720) pilA 2754830..2755351 (+) 522 WP_078222165.1 pilin Machinery gene
  ACEQ38_RS12725 (ACEQ38_12725) pilA 2755614..2756120 (+) 507 WP_075465281.1 pilin Machinery gene
  ACEQ38_RS12730 (ACEQ38_12730) - 2756197..2757933 (+) 1737 WP_078222166.1 PglL family O-oligosaccharyltransferase -
  ACEQ38_RS12735 (ACEQ38_12735) moaC 2758007..2758483 (-) 477 WP_078222167.1 cyclic pyranopterin monophosphate synthase MoaC -
  ACEQ38_RS12740 (ACEQ38_12740) - 2758578..2760266 (+) 1689 WP_078222168.1 M48 family metallopeptidase -

Sequence

Protein


Download         Length: 173 a.a.        Molecular weight: 17745.45 Da        Isoelectric Point: 10.2418

>NTDB_id=1045473 ACEQ38_RS12720 WP_078222165.1 2754830..2755351(+) (pilA) [Ralstonia syzygii subsp. celebesensis strain NCPPB 3727]
MASKHSRNRRRGQRGFTLIELMIVVAIIGILAAIAIPAYQDYTIRARVTEGLSLAAQAKALVVENAANAQSSLALGSASL
PASRNVSALNIDSSTGEITVVYASAVTTSGANTLVLTPYSGSSSALANLQAGSAPPAMVLWVCAAAGKSMPVTTVTQKTT
ATLPPKYAPAECR

Nucleotide


Download         Length: 522 bp        

>NTDB_id=1045473 ACEQ38_RS12720 WP_078222165.1 2754830..2755351(+) (pilA) [Ralstonia syzygii subsp. celebesensis strain NCPPB 3727]
ATGGCATCGAAGCACAGCAGGAACCGGCGGCGCGGCCAGCGCGGCTTTACATTGATTGAGTTGATGATCGTGGTGGCGAT
CATTGGGATATTGGCGGCGATTGCGATTCCGGCGTACCAGGACTACACGATCCGCGCGCGCGTGACCGAAGGTCTGTCGC
TGGCTGCGCAGGCCAAGGCGCTGGTGGTTGAGAATGCCGCCAATGCCCAGAGCAGCTTGGCACTCGGCTCCGCATCCTTG
CCGGCGAGCAGGAATGTCAGTGCTTTGAATATTGATTCGTCTACGGGCGAGATTACTGTGGTCTACGCGTCTGCTGTCAC
GACCAGCGGGGCGAACACGCTGGTCTTGACCCCATACTCCGGAAGTTCGTCGGCGCTGGCTAATTTGCAGGCTGGCTCGG
CACCGCCGGCAATGGTGCTGTGGGTGTGCGCTGCCGCCGGAAAGTCGATGCCGGTCACCACCGTCACCCAGAAAACGACG
GCTACGTTGCCACCGAAATACGCGCCTGCAGAGTGCCGTTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(45-173)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

54.545

100

0.555
  pilA2 Legionella pneumophila str. Paris

47.879

95.376

0.457
  pilA2 Legionella pneumophila strain ERS1305867

47.273

95.376

0.451
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.037

100

0.405
  comP Acinetobacter baylyi ADP1

42.236

93.064

0.393

Multiple sequence alignment