Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACEPM0_RS02680 Genome accession   NZ_CP168731
Coordinates   551942..553669 (-) Length   575 a.a.
NCBI ID   WP_197344961.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RUN6285     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 546942..558669
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPM0_RS02650 (ACEPM0_02650) - 547565..548026 (+) 462 WP_011002737.1 NUDIX domain-containing protein -
  ACEPM0_RS02655 (ACEPM0_02655) yacG 548019..548234 (-) 216 WP_011002736.1 DNA gyrase inhibitor YacG -
  ACEPM0_RS02660 (ACEPM0_02660) zapD 548245..549003 (-) 759 WP_016723794.1 cell division protein ZapD -
  ACEPM0_RS02665 (ACEPM0_02665) coaE 549121..549735 (-) 615 WP_011002734.1 dephospho-CoA kinase -
  ACEPM0_RS02670 (ACEPM0_02670) - 549748..550629 (-) 882 WP_029240316.1 A24 family peptidase -
  ACEPM0_RS02675 (ACEPM0_02675) pilC 550632..551897 (-) 1266 WP_019718222.1 type II secretion system F family protein Machinery gene
  ACEPM0_RS02680 (ACEPM0_02680) pilB 551942..553669 (-) 1728 WP_197344961.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACEPM0_RS02685 (ACEPM0_02685) - 553917..555194 (-) 1278 WP_165591155.1 HlyC/CorC family transporter -
  ACEPM0_RS02695 (ACEPM0_02695) ispB 555482..556450 (-) 969 WP_011002729.1 octaprenyl diphosphate synthase -
  ACEPM0_RS02700 (ACEPM0_02700) rplU 556729..557040 (+) 312 WP_011002728.1 50S ribosomal protein L21 -
  ACEPM0_RS02705 (ACEPM0_02705) rpmA 557078..557338 (+) 261 WP_003271630.1 50S ribosomal protein L27 -
  ACEPM0_RS02710 (ACEPM0_02710) obgE 557515..558615 (+) 1101 WP_011002727.1 GTPase ObgE -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63740.58 Da        Isoelectric Point: 7.1546

>NTDB_id=1045356 ACEPM0_RS02680 WP_197344961.1 551942..553669(-) (pilB) [Ralstonia pseudosolanacearum strain RUN6285]
MTLGLSLAQSRRIAPTLLTQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDTVPPVLSANRHF
QQLRLLPLGRRDNRIIVATSDPSDPKPLEELRQKMNVAIETVIVEHDKLVRQLSLSNEAPAEIKSLIPKQEATQIEYDPG
AAAAVRRTPTDGIDDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILEQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDKNDKDNKEMVERAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNKLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKACKKPGPLPKETL
LDAGFKEADLDGSWQPYHPVGCEACNGSGYKGRVGIYQVMPITEAIQEIILAHGTALQIAEQARKEGVLSLRDSGLRKVK
QGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=1045356 ACEPM0_RS02680 WP_197344961.1 551942..553669(-) (pilB) [Ralstonia pseudosolanacearum strain RUN6285]
ATGACTCTCGGACTCTCTCTTGCGCAGAGCCGGCGAATTGCGCCGACATTACTCACCCAGCTAGAACAAAGCGCCCGCGA
AAAGCGCACGCAGCTGATCGACGAACTGGTGAGCAGCGGCATCATGAGCGCTCATGATCTCGCCGTCTTCGTGGCGGGCA
AATACCAGATGCCGCTGCTGGACCTGGCGCAATACAAGCTCGACACCGTGCCGCCTGTGCTGTCGGCCAACAGGCATTTT
CAGCAGTTGCGCCTGTTGCCGCTGGGCCGGCGTGACAACCGCATCATCGTGGCCACTTCGGACCCGAGCGACCCCAAGCC
GCTCGAGGAACTGCGCCAGAAGATGAACGTGGCGATCGAGACGGTCATCGTCGAGCACGACAAGCTGGTGCGCCAGCTGA
GCCTCTCCAACGAGGCACCGGCCGAGATCAAGTCGCTCATCCCCAAGCAGGAAGCCACGCAGATCGAATACGACCCGGGT
GCGGCCGCGGCGGTCCGCCGCACCCCGACCGACGGCATCGACGATGCGCCGGTGGTGCGCTTCCTGCAGAAGCTGTTTAC
CGAGGCGCTGCTGCGCGGCACCTCCGACTTGCATTTCGAGCCGTTTGAAACGTTTTACCGCGTGCGCTTCCGCATCGACG
GCATCCTCGAGCAGGTCGCGCAGCCGCCGCTGGACATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTGTCGCGCCTG
GACATATCGGAAAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTCCTGATCACCGTTCCGAAAGACAAGAACGACAAGGA
CAACAAGGAGATGGTCGAGCGCGCCATCGACTTCCGGGTGTCGACGCTGCCGACGCTCTTCGGCGAGAAGATCGTGATCC
GGATTCTGGAGTCCTCGACCGAGCGGCTCGATATCGACCAGCTCGGCTATGAGCCCGAGCAGAAGCAGCTCCTGCTGGAC
GTGATCAAGCGCCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTAGCGGCAAGACGGTGTCGCTGTACACCTTCCT
GAACAAGCTGAACCAGGGCGACATCAACATTTCCACCGCGGAAGACCCGGCGGAAATCCAGTTGCCCGGCATCAACCAGG
TCAACGTCAACGACAAGGCGGGGCTGACCTTCGCCGCGGCGCTGAAGTCCTTTCTGCGGCAGGATCCCGACATCATCATG
GTGGGCGAAATCCGGGACCTGGAAACCGCCGACATCTCCATCAAGGCCGCGCAGACCGGCCACTTGGTGTTCTCCACGCT
GCACACCAACGACGCACCGACCACGCTGACACGCCTGATGAACATGGGCGTGGCGCCGTTCAACATCGCATCGTCGGTGC
TGATGATCACGGCGCAGCGTCTGGGGCGGCGCCTGTGCAAGGCCTGCAAGAAGCCGGGTCCACTGCCCAAGGAAACGTTG
CTGGACGCGGGCTTCAAGGAAGCAGACCTGGACGGCTCGTGGCAGCCGTACCATCCGGTCGGCTGCGAGGCATGCAACGG
CAGCGGCTACAAGGGCCGAGTCGGCATCTACCAGGTCATGCCGATCACCGAGGCGATCCAGGAGATCATCCTCGCCCACG
GCACGGCGCTGCAGATCGCCGAACAGGCGCGCAAGGAAGGCGTGCTCTCGCTGCGCGATTCCGGCCTGCGGAAAGTGAAG
CAGGGGCTGACTTCCCTTGAGGAAGTGTTGGCCACGACCAACCAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-131)

Type II/IV secretion system protein (T2SSE)

(184-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

51.06

98.435

0.503
  pilF Neisseria gonorrhoeae MS11

53.148

93.913

0.499
  pilB Acinetobacter baylyi ADP1

49.554

97.565

0.483
  pilB Legionella pneumophila strain ERS1305867

47.163

98.087

0.463
  pilB Vibrio cholerae strain A1552

44.964

96.696

0.435
  pilB Vibrio campbellii strain DS40M4

44.649

94.261

0.421
  pilB Vibrio parahaemolyticus RIMD 2210633

44.649

94.261

0.421
  pilB/pilB1 Synechocystis sp. PCC 6803

35.574

100

0.377
  pilF Thermus thermophilus HB27

38.256

97.739

0.374

Multiple sequence alignment