Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACEPM3_RS03015 Genome accession   NZ_CP168727
Coordinates   642189..642695 (+) Length   168 a.a.
NCBI ID   WP_011000517.1    Uniprot ID   Q8Y1X9
Organism   Ralstonia pseudosolanacearum strain RUN6499     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 637189..647695
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPM3_RS02990 (ACEPM3_02990) - 637534..638160 (+) 627 WP_011000512.1 DUF2889 domain-containing protein -
  ACEPM3_RS02995 (ACEPM3_02995) sucC 638227..639393 (+) 1167 WP_011000513.1 ADP-forming succinate--CoA ligase subunit beta -
  ACEPM3_RS03000 (ACEPM3_03000) sucD 639478..640359 (+) 882 WP_011000514.1 succinate--CoA ligase subunit alpha -
  ACEPM3_RS03005 (ACEPM3_03005) - 640509..641207 (+) 699 WP_019717569.1 TerC family protein -
  ACEPM3_RS03010 (ACEPM3_03010) pilA 641401..641922 (+) 522 WP_247417620.1 pilin Machinery gene
  ACEPM3_RS03015 (ACEPM3_03015) pilA 642189..642695 (+) 507 WP_011000517.1 pilin Machinery gene
  ACEPM3_RS03020 (ACEPM3_03020) - 642777..644510 (+) 1734 WP_247417622.1 PglL family O-oligosaccharyltransferase -
  ACEPM3_RS03025 (ACEPM3_03025) tnpA 644673..645134 (+) 462 WP_405126711.1 IS66-like element accessory protein TnpA -
  ACEPM3_RS03030 (ACEPM3_03030) tnpB 645131..645484 (+) 354 WP_405126712.1 IS66 family insertion sequence element accessory protein TnpB -
  ACEPM3_RS03035 (ACEPM3_03035) tnpC 645517..647070 (+) 1554 WP_405126713.1 IS66 family transposase -

Sequence

Protein


Download         Length: 168 a.a.        Molecular weight: 17053.72 Da        Isoelectric Point: 9.9749

>NTDB_id=1045320 ACEPM3_RS03015 WP_011000517.1 642189..642695(+) (pilA) [Ralstonia pseudosolanacearum strain RUN6499]
MKSMRHLNKRVQKGFTLIELMIVVAIVGILAAIAIPAYQDYTVRARVTEGLSLAAQAKALVSENAANAQSDLSVGSSVFT
PTKNVANLTIAGTGTVPGQITVTYTTAAGGGTLALVPTAAGTALPVSSAPSGPIMWTCYAQNKAQAASSVAPSGTMSLAA
KYAPAECR

Nucleotide


Download         Length: 507 bp        

>NTDB_id=1045320 ACEPM3_RS03015 WP_011000517.1 642189..642695(+) (pilA) [Ralstonia pseudosolanacearum strain RUN6499]
ATGAAGTCGATGCGTCATCTCAACAAGCGTGTCCAGAAGGGTTTCACGCTGATCGAACTGATGATCGTGGTCGCGATCGT
CGGTATTCTGGCTGCGATTGCCATTCCGGCCTATCAGGACTACACGGTTCGTGCACGCGTGACGGAAGGTCTGTCGCTGG
CTGCGCAAGCCAAGGCGCTGGTGTCGGAAAACGCTGCCAATGCACAGTCTGACCTGTCGGTGGGTTCGTCGGTGTTCACG
CCCACCAAGAACGTTGCCAACTTGACGATTGCTGGTACGGGTACTGTTCCGGGTCAGATCACCGTCACCTATACGACGGC
GGCTGGCGGCGGCACGCTGGCTCTGGTTCCGACTGCGGCTGGTACTGCACTGCCGGTTAGCTCGGCACCGTCGGGCCCGA
TCATGTGGACCTGCTACGCTCAGAATAAGGCTCAGGCTGCTAGCAGCGTTGCTCCGAGCGGTACGATGTCGCTGGCAGCG
AAGTATGCTCCGGCAGAATGCCGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(43-168)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8Y1X9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

100

100

1
  pilA2 Legionella pneumophila strain ERS1305867

50.311

95.833

0.482
  pilA2 Legionella pneumophila str. Paris

49.689

95.833

0.476
  comP Acinetobacter baylyi ADP1

44.444

100

0.452
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36.082

100

0.417

Multiple sequence alignment