Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACEPM3_RS03010 Genome accession   NZ_CP168727
Coordinates   641401..641922 (+) Length   173 a.a.
NCBI ID   WP_247417620.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RUN6499     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 636401..646922
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPM3_RS02985 (ACEPM3_02985) recX 636780..637259 (+) 480 WP_011000511.1 recombination regulator RecX -
  ACEPM3_RS02990 (ACEPM3_02990) - 637534..638160 (+) 627 WP_011000512.1 DUF2889 domain-containing protein -
  ACEPM3_RS02995 (ACEPM3_02995) sucC 638227..639393 (+) 1167 WP_011000513.1 ADP-forming succinate--CoA ligase subunit beta -
  ACEPM3_RS03000 (ACEPM3_03000) sucD 639478..640359 (+) 882 WP_011000514.1 succinate--CoA ligase subunit alpha -
  ACEPM3_RS03005 (ACEPM3_03005) - 640509..641207 (+) 699 WP_019717569.1 TerC family protein -
  ACEPM3_RS03010 (ACEPM3_03010) pilA 641401..641922 (+) 522 WP_247417620.1 pilin Machinery gene
  ACEPM3_RS03015 (ACEPM3_03015) pilA 642189..642695 (+) 507 WP_011000517.1 pilin Machinery gene
  ACEPM3_RS03020 (ACEPM3_03020) - 642777..644510 (+) 1734 WP_247417622.1 PglL family O-oligosaccharyltransferase -
  ACEPM3_RS03025 (ACEPM3_03025) tnpA 644673..645134 (+) 462 WP_405126711.1 IS66-like element accessory protein TnpA -
  ACEPM3_RS03030 (ACEPM3_03030) tnpB 645131..645484 (+) 354 WP_405126712.1 IS66 family insertion sequence element accessory protein TnpB -

Sequence

Protein


Download         Length: 173 a.a.        Molecular weight: 17786.51 Da        Isoelectric Point: 10.2418

>NTDB_id=1045319 ACEPM3_RS03010 WP_247417620.1 641401..641922(+) (pilA) [Ralstonia pseudosolanacearum strain RUN6499]
MASKHSRNRRRGQRGFTLIELMIVVAIIGILAALAIPAYQDYTIRSRVIEGLALAGQAKALVVENAANAQSSLSLGSASL
PASKNVSALNIDSSTGEIAVVYAPAVTGAGANTLVLTPYSGSSSVFGNLQAGSTPPAMVLWVCAAAGKSMPVNTVTQRTT
ATLPPKYAPSECR

Nucleotide


Download         Length: 522 bp        

>NTDB_id=1045319 ACEPM3_RS03010 WP_247417620.1 641401..641922(+) (pilA) [Ralstonia pseudosolanacearum strain RUN6499]
ATGGCATCGAAGCACAGCAGGAACAGGCGGCGTGGCCAGCGCGGCTTTACATTGATCGAGTTGATGATCGTGGTGGCGAT
CATCGGGATTTTGGCGGCGCTGGCGATTCCGGCGTATCAGGACTACACGATCCGGTCGCGTGTGATCGAGGGGCTGGCAC
TGGCAGGGCAAGCCAAGGCGCTGGTGGTTGAAAATGCCGCCAACGCACAGAGTAGCCTGTCACTCGGTTCCGCATCGTTG
CCGGCCAGCAAGAACGTCAGTGCGCTGAATATTGATTCGTCCACCGGCGAAATCGCAGTGGTCTATGCACCTGCTGTTAC
GGGAGCGGGGGCGAATACGTTGGTATTGACCCCATACTCCGGCAGTTCGTCAGTATTTGGCAATTTGCAGGCAGGTTCAA
CGCCGCCTGCAATGGTGCTGTGGGTGTGTGCCGCCGCCGGCAAGTCGATGCCGGTCAATACCGTTACCCAGAGGACGACG
GCTACGTTGCCACCGAAATACGCGCCCTCGGAGTGCCGTTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-35)

Pilin (bacterial filament) (Pilin)

(45-173)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

51.705

100

0.526
  pilA2 Legionella pneumophila str. Paris

44.848

95.376

0.428
  pilA2 Legionella pneumophila strain ERS1305867

44.242

95.376

0.422
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.263

100

0.387
  comP Acinetobacter baylyi ADP1

40.741

93.642

0.382

Multiple sequence alignment